Implication of adenylyl cyclase 8 in pathological smooth muscle cell migration occurring in rat and human vascular remodelling

2010 ◽  
Vol 221 (3) ◽  
pp. 331-342 ◽  
Author(s):  
Marie Gueguen ◽  
Zela Keuylian ◽  
Véronique Mateo ◽  
Nathalie Mougenot ◽  
Anne-Marie Lompré ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cell ◽  
Adenylyl Cyclase ◽  
Muscle Cell ◽  
Vascular Remodelling ◽  
Smooth Muscle Cell Migration ◽  
Adenylyl Cyclase 8

The Migration of Human Smooth Muscle Cells In Vitro Is Mediated by Plasminogen Activation and Can Be Inhibited by α2-Macroglobulin Receptor Associated Protein

1997 ◽  
Vol 78 (02) ◽  
pp. 880-886 ◽  
Author(s):  
Monique J Wijnberg ◽  
Paul H A Quax ◽  
Nancy M E Nieuwenbroek ◽  
Jan H Verheijen
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Ldl Receptor ◽  
Migration And Invasion ◽  
Invasion Assay ◽  
Plasminogen Activation ◽  
Smooth Muscle Cell Migration

SummaryThe plasminogen activation system is thought to be important in cell migration processes. A role for this system during smooth muscle cell migration after vascular injury has been suggested from several animal studies. However, not much is known about its involvement in human vascular remodelling. We studied the involvement of the plasminogen activation system in human smooth muscle cell migration in more detail using an in vitro wound assay and a matrix invasion assay. Inhibition of plasmin activity or inhibition of urokinase-type plasminogen activator (u-PA) activity resulted in approximately 40% reduction of migration after 24 h in the wound assay and an even stronger reduction (70-80%) in the matrix invasion assay. Migration of smooth muscle cells in the presence of inhibitory antibodies against tissue-type plasminogen activator (t-PA) was not significantly reduced after 24 h, but after 48 h a 30% reduction of migration was observed, whereas in the matrix invasion assay a 50% reduction in invasion was observed already after 24 h. Prevention of the interaction of u-PA with cell surface receptors by addition of soluble u-PA receptor or α2-macroglobulin receptor associated protein (RAP) to the culture medium, resulted in a similar inhibition of migration and invasion. From these results it can be concluded that both u-PA and t-PA mediated plasminogen activation can contribute to in vitro human smooth muscle cell migration and invasion. Furthermore, the interaction between u-PA and its cell surface receptor appears also to be involved in this migration and invasion process. The inhibitory effects on migration and invasion by the addition of RAP suggests an involvement of a RAP sensitive receptor of the LDL receptor family, possibly the LDL-receptor related protein (LRP) and/or the VLDL receptor.

Plasminogen activator inhibitor-1 deficiency enhances flow-induced smooth muscle cell migration

2004 ◽  
Vol 114 (1) ◽  
pp. 57-65 ◽  
Author(s):  
John P Cullen ◽  
Suzanne M Nicholl ◽  
Shariq Sayeed ◽  
James V Sitzmann ◽  
S.Steve Okada ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cell ◽  
Plasminogen Activator ◽  
Muscle Cell ◽  
Plasminogen Activator Inhibitor ◽  
Plasminogen Activator Inhibitor 1 ◽  
Smooth Muscle Cell Migration ◽  
Activator Inhibitor

scholarly journals Unexpected Role of the Copper Transporter ATP7A in PDGF-Induced Vascular Smooth Muscle Cell Migration

2010 ◽  
Vol 107 (6) ◽  
pp. 787-799 ◽  
Author(s):  
Takashi Ashino ◽  
Varadarajan Sudhahar ◽  
Norifumi Urao ◽  
Jin Oshikawa ◽  
Gin-Fu Chen ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Copper Transporter ◽  
Smooth Muscle Cell Migration

scholarly journals Hepatoma-Derived Growth Factor Is Expressed after Vascular Injury in the Rat and Stimulates Smooth Muscle Cell Migration

2006 ◽  
Vol 59 (6) ◽  
pp. 778-783 ◽  
Author(s):  
Jill V Narron ◽  
Tamara D Stoops ◽  
Kurt Barringhaus ◽  
Martin Matsumura ◽  
Allen D Everett
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Growth Factor ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Injury ◽  
Smooth Muscle Cell Migration
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