Posting maternal blood samples for free β-human chorionic gonadotrophin testing

1995 ◽  
Vol 15 (9) ◽  
pp. 879-880 ◽  
Author(s):  
H. S. Cuckle ◽  
R. G. Jones
Keyword(s):  
Maternal Blood ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Blood Samples

CORPUS LUTEUM ACTIVITY AND PROSTAGLANDIN LEVELS AFTER PARTURITION IN COWS WITH RETAINED FETAL MEMBRANES

1987 ◽  
Vol 67 (1) ◽  
pp. 21-26 ◽  
Author(s):  
PIERRE MATTON ◽  
VICTOR ADELAKOUN ◽  
JACQUES DUFOUR
Keyword(s):  
Key Words ◽  
Corpus Luteum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Fetal Membranes ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Retained Placenta ◽  
Progesterone Production ◽  
Prostaglandin F

Previous results have shown that progesterone levels were higher on the day of parturition in cows with retained fetal membranes (RFM) than in cows with normal calving, suggesting incomplete lysis of the corpus luteum (CL). This experiment was performed to evaluate the activity of the CL and the level of 13,14-dihydro-15-keto prostaglandin F2α (PGFM) in RFM cows. Cows with RFM or those calving normally (NC) were ovariectomized 12–14 h after parturition. Blood samples were taken from the caudal and utero-ovarian veins. Slices of CL were incubated with or without human chorionic gonadotrophin (hCG) medium for 3 h. Plasma progesterone was higher in both the caudal and utero-ovarian veins of RFM cows than in those of NC cows (1.12 ± 0.25 vs. 0.62 ± 0.08 ng mL−1 and 2.4 ± 0.3 vs. 1.44 ± 0.33 ng mL−1, respectively). PGFM was also significantly higher in RFM cows (3.62 ± 0.19 vs. 2.55 ± 0.15 ng mL−1). Progesterone production by CL slices from both types of cows, incubated without hCG, was similar (65 ± 4.2 vs. 73 ± 5.1 μg g−1); with hCG, however, the progesterone production by the CL of RFM cows was 186.3 ± 10.7 μg g−1, 75.7 μg g−1 more than in CL of cows with normal calving. These results support the hypothesis of an incomplete luteolysis of the CL in RFM cows in spite of hieher levels of PGF2α. Key words: Corpus luteum activity, progesterone, prostaglandin, postpartum cows, retained placenta

PLASMA DIHYDROTESTOSTERONE IN NORMAL ADULT MALES AND ITS RELATION TO TESTOSTERONE

1975 ◽  
Vol 79 (2) ◽  
pp. 357-365 ◽  
Author(s):  
Karl M. Pirke ◽  
Peter Doerr
Keyword(s):  
Normal Values ◽  
Control Level ◽  
Free Ligand ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Adult Males ◽  
Blood Samples ◽  
Old Men ◽  
Layer Chromatography ◽  
Close Parallelism

ABSTRACT A radioimmunoassay for dihydrotestosterone (DHT) in plasma was developed using an antiserum raised against testosterone-3-oxime-bovine-serum-albumin. After extraction of 1 ml male plasma with diethylether, DHT was separated from testosterone (T) by thin-layer chromatography. A dextran-charcoal-suspension was used for the separation of bound and free ligand. The inter-assay variability was 10.4 % (C. V.) and the detection limit 1.77 ng/100 ml. The accuracy of the method as determined by mass recoveries and the specificity were shown to be satisfactory. Normal values were obtained in 45 young to middle-aged (22–61 years) and 37 old (68–93 years) men. The median and the 95 percentiles were 20.5–51.9–76.3 (ng/100 ml) and 19.5–50.9–101.5 (ng/100 ml) respectively. While DHT did not change in old age T fell by 20.6%. DHT and T showed a significant correlation: rS = 0.426, P < 0.01 (young men), rS = 0.752, P < 0.001 (old men). After 3 daily im injections of 5000 IU human chorionic gonadotrophin (HCG), DHT increased 1.50 times (range: 1.15–2.09, n = 12), T 1.86 times (range: 1.20–2.91, n = 12). After 4 daily administrations of 40 mg fluoxymesterone DHT fell to 29.6% of the control level (range: 16.0–48.2%, n = 12). Blood samples were obtained from a 24 year old man every 15 min for 24 h. A close parallelism was observed between the concentrations of DHT and T in the plasma.

Decrease in serum sex hormone binding globulin during human chorionic gonadotrophin stimulation in prepubertal boys

1985 ◽  
Vol 109 (3) ◽  
pp. 423-427 ◽  
Author(s):  
L. Dunkel
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Testicular Descent ◽  
Hormone Binding ◽  
Blood Samples ◽  
Hypogonadotrophic Hypogonadism ◽  
Temporal Relationships ◽  
Prepubertal Boys

Abstract. Temporal relationships between steroidogenic and sex hormone binding globulin (SHBG) responses to hCG were studied in 27 prepubertal boys: 19 with incomplete testicular descent and 8 with hypogonadotrophic hypogonadism (HH). Nine of the boys with incomplete testicular descent were given a single im injection of hCG and blood samples were taken daily for 5 days. Six of them showed a slight decrease in SHBG concentration by day 5. All the other 18 boys were given four im injections of hCG on days 0, 4, 7 and 10. Blood was taken before each injection and on day 14. In the boys with incomplete testiscular descent SHBG concentration decreased by day 14 (P < 0.01). All the boys with HH had an impaired testosterone response to hCG, and SHBG levels did not decrease after hCG. In only 2 of these boys SHBG concentrations were > 10% below the basal by day 14. These boys, however, also had the highest testosterone responses of their group. Thus it appears that if testosterone increases in prepubertal boys, SHBG decreases.

INCIDENCE AND RATE OF APPEARANCE AND DISAPPEARANCE OF ANTIGONADOTROPHIN IN THE BLOOD OF PATIENTS TREATED WITH PREGNANT MARES' SERUM GONADOTROPHIN

1964 ◽  
Vol 45 (4_Suppl) ◽  
pp. S235-S242 ◽  
Author(s):  
Erling Østergaard
Keyword(s):  
Effective Treatment ◽  
Subsequent Treatment ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Present Series ◽  
Human Pituitary ◽  
Continuous Therapy ◽  
Blood Samples ◽  
Intensive Courses ◽  
Blood Specimens

ABSTRACT Thirty-eight patients with amenorrhoea were treated with repeated series of injections of pregnant mares' serum gonadotrophin + human chorionic gonadotrophin. Each course consisted of 5 injections of 1500 IU or 3000 IU of serum gonadotrophin, Antex®, followed by 3 injections of 1500 IU or 3000 IU of chorionic gonadotrophin, Physex®, If required, this treatment was repeated at a few months' intervals, in some cases up to 5 times. After each course, the blood was studied for the presence of antigonadotrophin to Antex. Blood specimens were drawn in all cases 21 days after the last injection of Antex. Antigonadotrophin was found to be present in one out of 15 patients after the 1st course, in 8 out of 20 after the 2nd course, in 13 of 16 after the 3rd, and in 6 of 6 patients after the 4th and 5th courses. Progonadotrophic activity was demonstrated in the blood of 6 patients after the 1st course, in 4 after the 2nd course, and in one on the 10th day after the 3rd course. In 6 cases blood samples were obtained also immediately before, during and immediately after the treatment. Three showed, during the 3rd, 4th and 5th course respectively, antigonadotrophin in the blood even before the last injection of Antex. These courses were ineffective, and no patient got menstrual bleeding as a result of the 4th or 5th course. Some patients developed bleeding after the 3rd treatment, although antigonadotrophin was demonstrated in the blood on the 21st day. In these cases the antigonadotrophin presumably did not form until the injections of Antex had exerted their effect. The antigonadotrophin occurring during these therapeutic courses inactivates only serum gonadotrophin and limits effective treatment by this gonadotrophin to about 4 weeks in continuous therapy and to 3 of the brief, intensive courses employed in the present series. Apart from this, the formation of this antigonadotrophin did not have harmful consequences. It disappears from the blood within a period of a few months, occasionally up to about a year, and it does not prevent subsequent treatment with human pituitary gonadotrophin from being effective.

scholarly journals Study the association of human chorionic gonadotrophin in prediction of preeclampsia and its severity

2018 ◽  
Vol 6 (2) ◽  
pp. 135-144
Author(s):  
Basima Sh. Alghazali ◽  
Ashtha Farook Aboud
Keyword(s):  
Maternal Serum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Control Group ◽  
P Value ◽  
Blood Samples ◽  
Serum Free ◽  
Mild Preeclampsia ◽  
Β Hcg ◽  
Significant Increment

The objective of this study is to evaluate the potential clinical use of maternal serum free human chorionic gonadotrophine (β-hCG) in prediction of preeclampsia and its severity. Two hundred and ten blood samples were collected from patients. Twenty seven patients were developed PE. These patients were followed for up to five months (first reading at 16-20 week, second reading at 21-28 week and third reading at 29-40 week). Patients suffered from any other disease were not included in the current study. The control group consisted of one hundred and eighty subjects. They were pregnant women without preeclampsia and other complications. These patients also were followed for up to five months (first reading at 16-20 week, second reading at 21-28 week and third reading at 29-40 week). Three patients were escaped. Compared with the control, The elevation of serum β-hCG was statistically significant, P value (<0.001) in women who were developed preeclampsia (mild and sever preeclampsia) later on throughout their pregnancy at 16-20, 21-28, and at 29-40 weeks of gestation, and there is further significant increment in the level of serum β-hCG in women who develop sever preeclampsia when compared with women who develop mild preeclampsia throughout their pregnancy, p value (<0.001). We are concluded that serum β-hCG is significantly associated with preeclampsia and they can be used as a markers for prediction of preeclampsia early in pregnancy and for evaluation of its severity.

THE PRECISION OF THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1963 ◽  
Vol 43 (1) ◽  
pp. 155-160
Author(s):  
Jørgen Falck Larsen ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Clinical Analysis ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

ABSTRACT Various modifications of the Parlow test for luteinizing hormone (ovarian ascorbic acid depletion in rats) were tried. Human chorionic gonadotrophin was used instead of hypophyseal luteinizing hormone. The precision of the method was found to be so low, however, that the test could not be used for routine clinical analysis. The low precision found in this and other laboratories is thought to be due to the strains of rats used.

STUDIES ON THE AROMATISATION OF NEUTRAL STEROIDS IN PREGNANT WOMEN

1964 ◽  
Vol 45 (4) ◽  
pp. 535-559 ◽  
Author(s):  
E. Bolté ◽  
S. Mancuso ◽  
G. Eriksson ◽  
N. Wiqvist ◽  
E. Diczfalusy
Keyword(s):  
Pregnant Women ◽  
Comparative Studies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Radioactive Material ◽  
Placental Barrier ◽  
Therapeutic Abortion ◽  
Limited Ability ◽  
Better Than

ABSTRACT In 15 cases of therapeutic abortion by laparotomy the placenta was disconnected from the foetus and perfused in situ with tracer amounts of radioactive dehydroepiandrosterone (DHA), dehydroepiandrosterone sulphate (DHAS), androst-4-ene-3,17-dione (A), testosterone (T) and 17β-oestradiol (OE2). Analysis of the placentas, perfusates and urine samples revealed an extensive aromatisation of DHA, A and T; more than 70% of the radioactive material recovered was phenolic, and at least 80 % of this phenolic material was identified as oestrone (OE1), 17β-oestradiol (OE2) and oestriol (OE3), the latter being detected only in the urine. Comparative studies indicated that A and T were aromatised somewhat better than DHA and that all three unconjugated steroids were aromatised to a much greater extent than DHAS. Radioactive OE1 and OE2 were isolated and identified in the placentas and perfusates, but no OE3, epimeric oestriols, or ring D ketols could be detected in these sources, not even when human chorionic gonadotrophin (HCG) was added to the blood prior to perfusion. Lack of placental 16-hydroxylation was also apparent when OE2 was perfused. Regardless of the precursor perfused, there was three times more OE2 than OE1 in the placenta and three times more OE1 than OE2 in the perfusate. This was also the case following perfusion with OE2. The results are interpreted as suggesting the existence in the pregnant human of a placental »barrier« limiting the passage of circulating androgen. The barrier consists of a) limited ability to transfer directly DHAS and b) an enzymic mechanism resulting in the rapid and extensive aromatisation of the important androgens DHA, A and T.

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