Expression profiling of proteins inL-threonine biosynthetic pathway ofEscherichia coli by using antibody microarray

PROTEOMICS ◽  
2006 ◽  
Vol 6 (22) ◽  
pp. 5929-5940 ◽  
Author(s):  
Min-Kyu Han ◽  
Mi-Young Hong ◽  
Dohoon Lee ◽  
Dong-Eun Lee ◽  
Geon Youp Noh ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Biosynthetic Pathway ◽  
Ofescherichia Coli ◽  
Antibody Microarray

Development ofEscherichia coli-based gene expression profiling of sewage sludge leachates

2018 ◽  
Vol 125 (5) ◽  
pp. 1502-1517 ◽  
Author(s):  
M. Goswami ◽  
F. A. Khan ◽  
A. Ibrisevic ◽  
P.-E. Olsson ◽  
J. Jass
Keyword(s):  
Gene Expression ◽  
Sewage Sludge ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Ofescherichia Coli

scholarly journals Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel

2015 ◽  
Vol 61 (2) ◽  
pp. 41-49
Author(s):  
Vladimír Repka ◽  
Roderik Fiala ◽  
Milada Čiamporová ◽  
Michal Martinka ◽  
Ján Pavlovkin
Keyword(s):  
Western Blot ◽  
Expression Profiling ◽  
Protein Extraction ◽  
Fluorescent Labelling ◽  
Antibody Microarray ◽  
Simultaneous Exposure ◽  
Maize Cultivars ◽  
Maize Roots ◽  
Microarray Expression Profiling ◽  
Microarray Expression

Abstract Here, we report the effectiveness of antibody microarray expression profiling (AbMEP) procedure to determine similarities and differences between two maize cultivars (Premia and Blitz) exposed to various concentration of cadmium (Cd), nickel (Ni), as well as simultaneous exposure to both metals (Cd + Ni) for 0, 12 and 24 h. After protein extraction from control (untreated) and heavy metals (HM)-treated root pairs and their fluorescent labelling, the protein extracts were used for the AbMEP procedure and western blot analysis. The results from the microarray were analysed using an internally normalised ratio. Using this highly parallel AbMEP-approach, the timing, dynamics and regulation of the expression of 101 specific genes in untreated and HM-treated roots of maize cultivars were determined. The microarray results revealed 23, 15 and 10 up-regulated/down-regulated proteins in Cd, Ni and Cd + Ni treated cv. Premia roots, respectively. In contrast, the microarray results revealed that 15, 11 and 7 proteins were up-regulated/down-regulated in Cd, Ni and Cd + Ni treated cv. Blitz roots, respectively. The data obtained from the AbMEP array experiment were validated by western blot analyses with more than 89% (the Pearson’s correlation coeficient Rr = 0.78) correlation between the two methods.

scholarly journals Effect of Enzyme Inhibitors on Terpene Trilactones Biosynthesis and Gene Expression Profiling in Ginkgo biloba Cultured Cells

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001
Author(s):  
Lijia Chen ◽  
Hui Tong ◽  
Mingxuan Wang ◽  
Jianhua Zhu ◽  
Jiachen Zi ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Expression Profiling ◽  
Biosynthetic Pathway ◽  
Enzyme Inhibitors ◽  
Cultured Cells ◽  
Mevalonic Acid ◽  
Specific Enzyme ◽  
Mva Pathway ◽  
Terpene Trilactones ◽  
Highly Correlated

The biosynthetic pathway of terpene trilactones of Ginkgo biloba is unclear. In this present study, suspension cultured cells of G. biloba were used to explore the regulation of the mevalonic acid (MVA) and methylerythritol 4-phosphate (MEP) pathways in response to specific enzyme inhibitors (lovastatin and clomazone). The results showed that the biosynthesis of bilobalide was more highly correlated with the MVA pathway, and the biosynthesis of ginkgolides was more highly correlated with the MEP pathway. Meanwhile, according to the results, it could be speculated that bilobalide might be a product of ginkgolide metabolism.

scholarly journals Cellulose production is coupled to sensing of the pyrimidine biosynthetic pathway via c-di-GMP production by the DgcQ protein ofEscherichia coli

2017 ◽  
Vol 19 (11) ◽  
pp. 4551-4563 ◽  
Author(s):  
Elio Rossi ◽  
Sara Motta ◽  
Alessandro Aliverti ◽  
Federica Cossu ◽  
Louise Gourlay ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Ofescherichia Coli ◽  
Cellulose Production ◽  
Gmp Production

scholarly journals Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining

2007 ◽  
Vol 7 (1) ◽  
pp. 163-169 ◽  
Author(s):  
X. Cynthia Song ◽  
Guanyuan Fu ◽  
Xufen Yang ◽  
Zhong Jiang ◽  
Yingjian Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Cancer Cells ◽  
Expression Profiling ◽  
Breast Cancer Cells ◽  
Antibody Microarray

Effect of Levopimaradiene on Terpene Trilactones Biosynthesis and Gene Expression Profiling in Ginkgo biloba Cells

2017 ◽  
Vol 12 (7) ◽  
pp. 1934578X1701200
Author(s):  
Jianhua Zhu ◽  
Pu Wang ◽  
Minghua Qian ◽  
Chuxin Liang ◽  
Jiachen Zi ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Expression Profiling ◽  
Biosynthetic Pathway ◽  
Mep Pathway ◽  
Ginkgolide B ◽  
Control Groups ◽  
Meristematic Cells ◽  
Qrt Pcr ◽  
Ginkgolide A ◽  
Terpene Trilactones

Ginkgolides (GKs) and Bilobalide (BB) are rare terpene trilactones obtained from Ginkgo biloba, but their biosynthetic pathway is still unclear. In this paper, effects of levopimaradiene (LP) on increasing the production of terpene trilactones of G. biloba dedifferentiated cells (DDCs) and cambial meristematic cells (CMCs) were reported. The productions of ginkgolide A (GA) and ginkgolide B (GB) were 1.61 and 1.32 folds larger than that of the control groups when G. biloba DDCs was treated with LP, and the productions of ginkgolide C (GC) and BB reached 234 and 161 μg L−1 after treated with LP for 60 h. The production of GA, GB, GC and BB was 2.03, 1.43, 1.22 and 1.19 folds larger than that of the control groups in LP-treated CMCs groups. The results demonstrated that BB could be produced from the methylerythritol 4-phosphate (MEP) pathway. qRT-PCR experiments showed that LP was a significant precursor manipulated the biosynthesis of terpene trilactones via affecting the transcript levels of several related genes in the MEP pathway.

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