Ananas comosus var. MD2 is currently the most preferred pineapple variety in the
international market due to its pleasant aroma and high Brix acidity ratio. In vitro
approaches such as callus culture is promising in producing disease-free plantlet.
However, there are limited studies reported on callus culture of MD2 variety despite the
potential of in vitro regeneration through biotechnological advances. The purpose of the
study was to determine the effect of plant growth regulators (PGRs) i.e., 2,4-
dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and Thidiazuron (TDZ)
on callus induction from leaf explant of MD2 pineapple. Leaf base explants were cultured
on Murashige and Skoog (MS) media supplemented with varying concentration of 2,4-D
(0.5 to 6.0 mg/L) alone and in combination with BAP (1.0 to 3.0 mg/L). The frequency of
callus induction was seen significantly highest (91.67±8.33%) with maximum callus fresh
weight (0.25±0.07 g) at a combination of 2.0 mg/L 2,4-D and 2.0 mg/L BAP. The shortest
duration of callus formation was seen on day 12 with the lowest concentration of 2,4-D,
0.5 mg/L. There is a moderate correlation between the earliness of callus formation and
the frequency of callus induction (P<0.01). The most favourable media for callus
proliferation was 6.0 mg/L 2,4-D and 2.0 mg/L TDZ as the highest fresh weight of
1.52±0.03 g was recorded. Callus culture has the potential to be a source of plant material
and secondary metabolites production. In this study, 2,4-D and BAP have successfully
induced callus in MD2 pineapple.
Effect of Enzyme Inhibitors on Terpene Trilactones Biosynthesis and Gene Expression Profiling in Ginkgo biloba Cultured Cells
