scholarly journals Continuous Hydrolysis of Starch in Membrane Unit Connected Immobilized Enzyme Reactor

10.5109/23926 ◽  
1989 ◽  
Vol 33 (3/4) ◽  
pp. 167-175
Author(s):  
Dudsadee Uttapap ◽  
Yojiro Koba ◽  
Ayaaki Ishizaki
Keyword(s):  
Immobilized Enzyme ◽  
Enzyme Reactor ◽  
Membrane Unit ◽  
Immobilized Enzyme Reactor ◽  
Hydrolysis Of

scholarly journals Hydrolysis of whey lactose by immobilized β-Galactosidase

2008 ◽  
Vol 51 (6) ◽  
pp. 1233-1240 ◽  
Author(s):  
Marcela Panaro Mariotti ◽  
Hideko Yamanaka ◽  
Angela Regina Araujo ◽  
Henrique Celso Trevisan
Keyword(s):  
Thermal Treatment ◽  
Aspergillus Oryzae ◽  
Immobilized Enzyme ◽  
Fixed Bed ◽  
Fixed Bed Reactor ◽  
Enzyme Reactor ◽  
Substrate Solution ◽  
Immobilized Enzyme Reactor ◽  
Commercial Use ◽  
Hydrolysis Of

Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.

scholarly journals Study of the geometry of open channels in a layer-bed-type microfluidic immobilized enzyme reactor

2021 ◽  
Author(s):  
Cynthia Nagy ◽  
Robert Huszank ◽  
Attila Gaspar
Keyword(s):  
Immobilized Enzyme ◽  
High Surface ◽  
Volume Ratio ◽  
Volumetric Flow Rate ◽  
Enzyme Reactor ◽  
Channel Pattern ◽  
Immobilized Enzyme Reactor ◽  
Split Flow ◽  
Parallel Streams ◽  
Enzymatic Microreactors

AbstractThis paper aims at studying open channel geometries in a layer-bed-type immobilized enzyme reactor with computer-aided simulations. The main properties of these reactors are their simple channel pattern, simple immobilization procedure, regenerability, and disposability; all these features make these devices one of the simplest yet efficient enzymatic microreactors. The high surface-to-volume ratio of the reactor was achieved using narrow (25–75 μm wide) channels. The simulation demonstrated that curves support the mixing of solutions in the channel even in strong laminar flow conditions; thus, it is worth including several curves in the channel system. In the three different designs of microreactor proposed, the lengths of the channels were identical, but in two reactors, the liquid flow was split to 8 or 32 parallel streams at the inlet of the reactor. Despite their overall higher volumetric flow rate, the split-flow structures are advantageous due to the increased contact time. Saliva samples were used to test the efficiencies of the digestions in the microreactors. Graphical abstract

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