scholarly journals In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. 437-447 ◽  
Author(s):  
Shibojyoti Lahiri ◽  
Na Sun ◽  
Victor Solis-Mezarino ◽  
Andreas Fedisch ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Histone Variants ◽  
In Situ Detection

Inside front cover: In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. NA-NA
Author(s):  
Shibojyoti Lahiri ◽  
Na Sun ◽  
Victor Solis-Mezarino ◽  
Andreas Fedisch ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Histone Variants ◽  
Front Cover ◽  
In Situ Detection

scholarly journals Region-specific bioconversion of dynorphin neuropeptide detected by in situ histochemistry and MALDI imaging mass spectrometry

Peptides ◽  
2017 ◽  
Vol 87 ◽  
pp. 20-27 ◽  
Author(s):  
Erik Bivehed ◽  
Robert Strömvall ◽  
Jonas Bergquist ◽  
Georgy Bakalkin ◽  
Malin Andersson
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Imaging Mass Spectrometry

scholarly journals Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism

2018 ◽  
Author(s):  
Eylan Yutuc ◽  
Roberto Angelini ◽  
Mark Baumert ◽  
Natalia Mast ◽  
Irina Pikuleva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Brain Function ◽  
Mass Spectrometry Imaging ◽  
Cholesterol Metabolism ◽  
Biologically Active ◽  
Wild Type ◽  
Tissue Slices ◽  
The Brain

AbstractDysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including cholesterol and its precursors and metabolites, are biologically active and important for proper brain function. However, spatial cholesterol metabolism in brain and the resulting sterol distributions are poorly defined. To better understand cholesterol metabolism in situ across the complex functional regions of brain, we have developed on-tissue enzyme-assisted derivatisation in combination with micro-liquid-extraction for surface analysis and liquid chromatography - mass spectrometry to image sterols in tissue slices (10 µm) of mouse brain. The method provides sterolomic analysis at 400 µm spot diameter with a limit of quantification of 0.01 ng/mm2. It overcomes the limitations of previous mass spectrometry imaging techniques in analysis of low abundance and difficult to ionise sterol molecules, allowing isomer differentiation and structure identification. Here we demonstrate the spatial distribution and quantification of multiple sterols involved in cholesterol metabolic pathways in wild type and cholesterol 24S-hydroxylase knock-out mouse brain. The technology described provides a powerful tool for future studies of spatial cholesterol metabolism in healthy and diseased tissues.SignificanceThe brain is a remarkably complex organ and cholesterol homeostasis underpins brain function. It is known that cholesterol is not evenly distributed across different brain regions, however, the precise map of cholesterol metabolism in the brain remains unclear. If cholesterol metabolism is to be correlated with brain function it is essential to generate such a map. Here we describe an advanced mass spectrometry imaging platform to reveal spatial cholesterol metabolism in situ at 400 µm resolution on 10 µm tissue slices from mouse brain. We mapped, not only cholesterol, but also other biologically active sterols arising from cholesterol turnover in both wild type and mice lacking cholesterol 24-hydroxylase (Cyp46a1), the major cholesterol metabolising enzyme.

scholarly journals Chlorine activation by N<sub>2</sub>O<sub>5</sub>: simultaneous, in situ detection of ClNO<sub>2</sub> and N<sub>2</sub>O<sub>5</sub> by chemical ionization mass spectrometry

2009 ◽  
Vol 2 (1) ◽  
pp. 119-151 ◽  
Author(s):  
J. P. Kercher ◽  
T. P. Riedel ◽  
J. A. Thornton
Keyword(s):  
Mass Spectrometry ◽  
Detection Limit ◽  
Chemical Ionization ◽  
Ionization Mass Spectrometry ◽  
Chemical Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Nitrate Anion ◽  
Detection Scheme ◽  
In Situ Detection

Abstract. We report a new method for the simultaneous in situ detection of nitryl chloride (ClNO2) and dinitrogen pentoxide (N2O5) using chemical ionization mass spectrometry (CIMS). The technique relies on the formation and detection of iodide ion-molecule clusters, I(ClNO2)− and I(N2O5)−. The novel N2O5 detection scheme is direct. It does not suffer from high and variable chemical interferences, which are associated with the typical method of nitrate anion detection. We address the role of water vapor, electric field strength, and instrument zero determinations, which influence the overall sensitivity and detection limit of this method. For both species, the method demonstrates high sensitivity (>1 Hz/pptv), precision (~10% for 100 pptv in 1 s), and accuracy (~20%), the latter ultimately determined by the nitrogen dioxide (NO2) cylinder calibration standard and characterization of inlet effects. For the typically low background signals (<10 Hz) and high selectivity, we estimate signal-to-noise (S/N) ratios of 2 for 1 pptv in 60 s averages, but uncertainty associated with the instrumental zero currently leads to an ultimate detection limit of ~5 pptv for both species. We validate our approach for the simultaneous in situ measurement of ClNO2 and N2O5 while on board the Research Vessel (RV) Knorr as part of the ICEALOT 2008 Field Campaign.

scholarly journals Three-dimensional visualization of protein expression in mouse brain structures using imaging mass spectrometry

2005 ◽  
Vol 16 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
Anna C. Crecelius ◽  
D. Shannon Cornett ◽  
Richard M. Caprioli ◽  
Betsy Williams ◽  
Benoit M. Dawant ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Expression ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Three Dimensional ◽  
Brain Structures
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