scholarly journals Differential stability of the bovine prion protein upon urea unfolding

2009 ◽  
Vol 18 (10) ◽  
pp. 2172-2182 ◽  
Author(s):  
Olivier Julien ◽  
Subhrangsu Chatterjee ◽  
Angela Thiessen ◽  
Steffen P. Graether ◽  
Brian D. Sykes
Keyword(s):  
Prion Protein ◽  
Differential Stability ◽  
Urea Unfolding

Quantitative ultrastructural reconstruction of small regions within large volumes by correlative light and high voltage electron microscopy of serial 0.25-0.50 μm sections

1987 ◽  
Vol 45 ◽  
pp. 578-581
Author(s):  
Conly L. Rieder ◽  
Frederick J. Miller ◽  
Edwin Davison ◽  
Samuel S. Bowser ◽  
Kirsten Lewis ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Mitotic Spindle ◽  
Meiotic Spindle ◽  
High Voltage Electron Microscopy ◽  
Male And Female ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Differential Stability ◽  
Sperm Aster

In this abstract we Illustrate how same-section correlative light and high voltage electron microscopy (HVEM) of serial 0.25-0.50-μm sections can answer questions which are difficult to approach by EM of 60-100 nm sections.Starfish (Pisaster and Asterlas) eggs are fertilized at meiosis I when the oocyte contains two maternal centrosomes (e.g., asters) which form the poles of the first meiotic spindle. Immediately after fertilization a sperm aster is assembled in the vicinity of the male pronucleus and persists throughout meiosis. At syngamy the sperm aster splits to form the poles of the first mitotic spindle. During this time the functional and replicative properties of the maternal centrosome, inherited from the last meiotic division, are lost. The basis for this differential stability, of male and female centrosomes in the same cytoplasm, is a mystery.

Metallic prions

2004 ◽  
Vol 71 ◽  
pp. 193-202 ◽  
Author(s):  
David R Brown
Keyword(s):  
Prion Protein ◽  
Binding Capacity ◽  
Normal Function ◽  
Transmissible Spongiform Encephalopathies ◽  
Published Data ◽  
Normal Brain ◽  
Copper Binding ◽  
Loss Of Function ◽  
Spongiform Encephalopathies ◽  
The Brain

Prion diseases, also referred to as transmissible spongiform encephalopathies, are characterized by the deposition of an abnormal isoform of the prion protein in the brain. However, this aggregated, fibrillar, amyloid protein, termed PrPSc, is an altered conformer of a normal brain glycoprotein, PrPc. Understanding the nature of the normal cellular isoform of the prion protein is considered essential to understanding the conversion process that generates PrPSc. To this end much work has focused on elucidation of the normal function and activity of PrPc. Substantial evidence supports the notion that PrPc is a copper-binding protein. In conversion to the abnormal isoform, this Cu-binding activity is lost. Instead, there are some suggestions that the protein might bind other metals such as Mn or Zn. PrPc functions currently under investigation include the possibility that the protein is involved in signal transduction, cell adhesion, Cu transport and resistance to oxidative stress. Of these possibilities, only a role in Cu transport and its action as an antioxidant take into consideration PrPc's Cu-binding capacity. There are also more published data supporting these two functions. There is strong evidence that during the course of prion disease, there is a loss of function of the prion protein. This manifests as a change in metal balance in the brain and other organs and substantial oxidative damage throughout the brain. Thus prions and metals have become tightly linked in the quest to understand the nature of transmissible spongiform encephalopathies.

Prion Protein

2017 ◽  
Keyword(s):  
Prion Protein

A Novel Protective Prion Protein Variant that Colocalizes with Kuru Exposure

2010 ◽  
Vol 2010 ◽  
pp. 73-74
Author(s):  
D. van de Beek
Keyword(s):  
Prion Protein ◽  
Protein Variant

CHARACTERISTICS OF ALLELE POOL OF THE ROMANOV SHEEP BREED FOR THE PRION PROTEIN GENE ASSOCIATED WITH GENETIC SUSTAINABILITY TO SCRAPIE

2017 ◽  
Vol 52 (6) ◽  
pp. 1157-1165
Author(s):  
E.A. Gladyr ◽  
◽  
T.E. Deniskova ◽  
V.A. Bagirov ◽  
O.V. Kostyunina ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Sheep Breed ◽  
Prion Protein Gene ◽  
Allele Pool

A Unique Functional Feature of the Recombinant Bovine Prion Protein Fragment

2014 ◽  
Author(s):  
Ying Zhai ◽  
Na Li ◽  
Dachuan Zhang ◽  
Qi Li ◽  
Guoping Zhou ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Fragment ◽  
Functional Feature
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