scholarly journals A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity

2011 ◽  
Vol 79 (4) ◽  
pp. 1034-1047 ◽  
Author(s):  
Daniel G. Isom ◽  
Philippe R. Marguet ◽  
Terrence G. Oas ◽  
Homme W. Hellinga

Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins

The present knowledge of the three-dimensional structure of ribosomes is far too limited to enable a complete understanding of the various roles which ribosomes play in protein biosynthesis. The spatial arrangement of proteins and ribonuclec acids in ribosomes can be analysed in many ways. Determination of binding sites for individual proteins on ribonuclec acid and locations of the mutual positions of proteins on the ribosome using labeling with fluorescent dyes, cross-linking reagents, neutron-diffraction or antibodies against ribosomal proteins seem to be most successful approaches. Structure and function of ribosomes can be correlated be depleting the complete ribosomes of some proteins to the functionally inactive core and by subsequent partial reconstitution in order to regain active ribosomal particles.



Author(s):  
Songlin Luo ◽  
Shoubin Chen ◽  
Jinguang Li
Keyword(s):  


2004 ◽  
Vol 18 (4) ◽  
pp. 665-668 ◽  
Author(s):  
M.M.H Huisman ◽  
A Oosterveld ◽  
H.A Schols


Biochemistry ◽  
2012 ◽  
Vol 51 (26) ◽  
pp. 5339-5347 ◽  
Author(s):  
Damien Farrell ◽  
Helen Webb ◽  
Michael A. Johnston ◽  
Thomas A. Poulsen ◽  
Fergal O’Meara ◽  
...  




2016 ◽  
Vol 8 (37) ◽  
pp. 6705-6710 ◽  
Author(s):  
Pengcheng Han ◽  
Daming Dong ◽  
Xiaofan Du ◽  
Leizi Jiao ◽  
Xiande Zhao

Laser-induced breakdown spectroscopy was used for the fast determination of calcium concentration in the internal tissues of a single seed. Compared with conventional methods, LIBS had obvious advantages, such as a little analysis spot, little ablation, high precision, and small influence on the seed vigor.





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