Phosphine resistance in Australian Cryptolestes species (Coleoptera: Laemophloeidae): perspectives from mitochondrial DNA cytochrome oxidase I analysis

2016 ◽  
Vol 72 (6) ◽  
pp. 1250-1259 ◽  
Author(s):  
Wee Tek Tay ◽  
Stephen J Beckett ◽  
Paul J De Barro
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Cytochrome Oxidase I ◽  
Phosphine Resistance

scholarly journals Genetic Diversity and Molecular Phylogeny of Iranian Goats Based on Cytochrome Oxidase I (COXI) Gene Sequences (KERAGAMAN GENETIK DAN FILOGENI MOLEKULER KAMBING-KAMBING IRAN BERDASARKAN SEKUENS GEN CYTOCHROME OXIDASE I (COXI))

2018 ◽  
Vol 18 (4) ◽  
pp. 565
Author(s):  
Reza Seyed Sharifi ◽  
Sima Savar Sofla ◽  
Hamid Reza Seyedabadi
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Phylogenetic Trees ◽  
Genomic Structure ◽  
Cytochrome Oxidase I ◽  
Conservation Strategies ◽  
Salting Out ◽  
Cytochrome Oxidase I Gene ◽  
The Rich ◽  
I Gene

Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNA Cytochrome oxidase subunit I (COXI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using Polymerase Chain Reaction (PCR) method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. A partial sequence of cytochrome oxidase I gene of Iranian goats is 1286 bp and contained four variable sites and three haplotypes. Phylogenetic analysis of haplotype in the combination with the goat from GenBank showed that Iranian goatclustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.

scholarly journals Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences

2018 ◽  
Author(s):  
Mohamad-Azam Akmal Abu Bakar ◽  
Jeffrine Japning Rovie-Ryan ◽  
Ahmad Ampeng ◽  
Salmah Yaakop ◽  
Shukor Md Nor ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Distance ◽  
Cytochrome Oxidase ◽  
Cytochrome Oxidase I ◽  
Loop Region ◽  
D Loop

scholarly journals Karakteristik Molekuler Kelelawar (Microchiroptera), berdasarkan DNA Mitokondria (Gen COI) di Gua Sukabumi dan Sentul Jawa Barat

2021 ◽  
Vol 14 (1) ◽  
pp. 20-28
Author(s):  
Ismayati Afifah ◽  
Dedy Duryadi Solihin ◽  
Arzyana Sunkar
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Distance ◽  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Cytochrome Oxidase I ◽  
Genetic Identity ◽  
Genetic Identification ◽  
Coi Gene ◽  
Genetic Characteristics ◽  
Spin Column

AbstrakCytochrome Oxidase I (COI) merupakan salah satu gen mitokondria untuk membantu konstruksi dari pohon filogeni yang dapat bertindak sebagai gen marker. Gen COI memiliki keakuratan dalam mengidentifikasi spesies dan umumnya digunakan sebagai “DNA Barcoding”. Informasi mengenai karakteristik genetik berdasarkan DNA mitokondria pada kelelawar di Sukabumi dan Sentul belum banyak dilaporkan. Tujuan dari penelitian ini untuk mengetahui keragaman genetik kelelawar berdasarkan DNA mitokondria dengan penanda Cytochrome Oxidase I (COI) sebagai DNA barcoding. Isolasi DNA total dilakukan menggunakan Kit Dneasy® Blood and Tissue Kit cat no 69504 (50) berdasarkan prosedur Spin-Column Protocol dengan modifikasi. Hasil penelitian ini menunjukkan bahwa gen COI telah berhasil mengidentifikasi karakteristik spesies. Dua haplotipe didapatkan dari masing-masing populasi. Berdasarkan barcode DNA menunjukkan populasi Sukabumi merupakan spesies Chaerephon plicatus dengan nilai identitas genetik sebesar 97,08%, sedangkan populasi Sentul menunjukkan perbedaan secara genetik dengan spesies Hipposideros larvatus dengan nilai identitas genetik sebesar 94,85%. Identifikasi secara genetik dengan menggunakan gen COI menunjukkan bahwa kelelawar yang berasal Sukabumi adalah spesies Chaerephon plicatus dengan jarak genetik sebesar 3,1%. Kelelawar yang berasal dari Sentul memiliki kedekatan dengan spesies Hipposideros larvatus namun memiliki jarak genetik sebesar 5,2%. AbstractCytochrome Oxidase I (COI) is one of the mitochondrial genes to help the construction of phylogeny trees that can act as marker genes. The COI gene has accuracy in identifying species and is commonly used as "DNA Barcoding". Information about genetic characteristics based on mitochondrial DNA in bats in Sukabumi and Sentul has not been widely reported. The purpose of this study was to determine the genetic diversity of bats based on Mitochondrial DNA with Cytochrome Oxidase I (COI) markers as DNA barcoding. Total DNA isolation was carried out using the Dneasy® Blood and Tissue Kit paint no. 69504 (50) based on the Spin-Column Protocol procedure with modifications. The results of this study indicate that the COI gene has successfully identified species characteristics. Two haplotypes were obtained from each population. Based on DNA barcodes, the population of Sukabumi is a species of Chaerephon plicatus with a genetic identity value of 97.08%, while the Sentul population shows genetic differences with the Hipposideros larvatus species with a genetic identity value of 94.85%. Genetic identification using the COI gene shows that the bats originating from Sukabumi is a spesies Chaerephon plicatus with a genetic distance of 3.1%. The bats originating from Sentul are closely related to the species Hipposideros larvatus but have a genetic distance of 5.2%.

Phylogenetic and biogeographic relationships of subterranean and surface genera of Australian Atyidae (Crustacea : Decapoda : Caridea) inferred with mitochondrial DNA

2007 ◽  
Vol 21 (2) ◽  
pp. 137 ◽  
Author(s):  
Timothy J. Page ◽  
Kristina von Rintelen ◽  
Jane M. Hughes
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
16S Rdna ◽  
Phylogenetic Relationships ◽  
Cytochrome Oxidase I ◽  
Freshwater Shrimp ◽  
Australian Species ◽  
The Family ◽  
Mitochondrial 16S Rdna ◽  
Disjunct Genus

The biogeographic and phylogenetic relationships of six of the eight Australian genera of freshwater shrimp from the family Atyidae were investigated using mitochondrial 16S rDNA and cytochrome oxidase I sequences. Previous studies on two of the epigean genera (Caridina, Paratya) indicate that Australian species have strong links to congenerics from outside, with Australian members of Paratya being monophyletic and Caridina polyphyletic. The present study found that the endemic Australian epigean genus Australatya forms a strong clade with Pacific ‘Atya-like’ genera (Atyoida, Atyopsis), and that the endemic Australian epigean genus Caridinides falls within a clade containing Caridina species from the Australian ‘indistincta’ group. The two hypogean genera included in this study (Parisia, Pycnisia) form a strong clade in all analyses, implying an Australian subterranean speciation. The possibility of a relationship between Parisia/Pycnisia and an Australian Caridina species may have implications for the monophyly of the highly disjunct genus Parisia (Australia, Madagascar, Philippines). Parisia may descend from local Caridina species and represent convergent morphologies.

Identification of forensically significant calliphorids based on mitochondrial DNA cytochrome oxidase I (COI) gene in China

2011 ◽  
Vol 207 (1-3) ◽  
pp. e64-e65 ◽  
Author(s):  
Qinlai Liu ◽  
Jifeng Cai ◽  
Yadong Guo ◽  
Xinghua Wang ◽  
Yan Gu ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Cytochrome Oxidase I ◽  
Coi Gene
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