Inhibitory effect of the carnosine-gallic acid synthetic peptide on MMP-2 and MMP-9 in human fibrosarcoma HT1080 cells

2014 ◽  
Vol 20 (9) ◽  
pp. 716-724 ◽  
Author(s):  
Sung-Rae Kim ◽  
Tae-Kil Eom ◽  
Hee-Guk Byun
Keyword(s):  
Gallic Acid ◽  
Synthetic Peptide ◽  
Inhibitory Effect ◽  
Ht1080 Cells

scholarly journals Development of a High-Throughput Method to Study the Inhibitory Effect of Phytochemicals on Trimethylamine Formation

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1466
Author(s):  
Lisard Iglesias-Carres ◽  
Lauren A. Essenmacher ◽  
Kathryn C. Racine ◽  
Andrew P. Neilson
Keyword(s):  
Chlorogenic Acid ◽  
Gallic Acid ◽  
High Throughput ◽  
Anaerobic Fermentation ◽  
Inhibitory Effect ◽  
Healthy Human ◽  
High Throughput Method ◽  
Lyase Activity ◽  
Inhibitory Potential ◽  
Potential Maximum

Choline is metabolized by the gut microbiota into trimethylamine (TMA), the precursor of pro-atherosclerotic molecule trimethylamine N-oxide (TMAO). A reduction in TMA formation has shown cardioprotective effects, and some phytochemicals may reduce TMA formation. This study aimed to develop an optimized, high-throughput anaerobic fermentation methodology to study the inhibition of choline microbial metabolism into TMA by phenolic compounds with healthy human fecal starter. Optimal fermentation conditions were: 20% fecal slurry (1:10 in PBS), 100 µM choline, and 12 h fermentation. Additionally, 10 mM of 3,3-dimethyl-1-butanol (DMB) was defined as a positive TMA production inhibitor, achieving a ~50% reduction in TMA production. Gallic acid and chlorogenic acid reported higher TMA inhibitory potential (maximum of 80–90% TMA production inhibition), with IC50 around 5 mM. Neither DMB nor gallic acid or chlorogenic acid reduced TMA production through cytotoxic effects, indicating mechanisms such as altered TMA-lyase activity or expression.

scholarly journals A New Noriridoid and Six Phenolic Compounds from Rhopalocnemis phalloides

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Nguyen Quang Hung ◽  
Nguyen Thi Luyen ◽  
Nguyen The Cuong ◽  
Tran Huy Thai ◽  
Nguyen Thanh Tung ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Protocatechuic Acid ◽  
Inhibitory Effect ◽  
Spectroscopic Methods ◽  
Hydroxybenzoic Acid ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
The Family

A rare noriridoid and six known phenolic compounds were isolated from the parasite plant Rhopalocnemis phalloides. Using spectroscopic methods, these compounds were identified as 10-acetoxy- cis-2-oxabicyclo[4.3.0]nonan-7-en-3-one (1), p-hydroxybenzoic acid (2), protocatechuic acid (3), gallic acid (4), coniferyl aldehyde (5), l- O-trans-cinnamoyl-β-D-glucoside (6), and coniferin (7). The noriridoid compound is the first reported from the family Balanophoraceae. Of the isolated compounds, coniferyl aldehyde had the strongest inhibitory effect on nitric oxide production (IC50 = 8.24 μM).

Inhibitory effect of methyl gallate and gallic acid on oral bacteria

2008 ◽  
Vol 46 (6) ◽  
pp. 744-750 ◽  
Author(s):  
Mi-Sun Kang ◽  
Jong-Suk Oh ◽  
In-Chol Kang ◽  
Suk-Jin Hong ◽  
Choong-Ho Choi
Keyword(s):  
Gallic Acid ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Methyl Gallate

Inhibitory effect of obovatal on the migration and invasion of HT1080 cells via the inhibition of MMP-2

2007 ◽  
Vol 15 (12) ◽  
pp. 4085-4090 ◽  
Author(s):  
Su-Kyung Lee ◽  
Hyo Kon Chun ◽  
Jae Young Yang ◽  
Dong Cho Han ◽  
Kwang-Hee Son ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Migration And Invasion ◽  
Ht1080 Cells

scholarly journals Flavonoids Bearing an O-Arabinofuranosyl-(1→3)-Rhamnoside Moiety from Cladocolea Micrantha: Inhibitory Effect on Human Melanoma Cells

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Anderson C. Guimarães ◽  
Alvicler Magalhães ◽  
Marcos J. Nakamura ◽  
Antonio C. Siani ◽  
Christina Barja-Fidalgo ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Spectral Methods ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
Derivatives Of ◽  
Transwell System

Eleven known triterpenes (α-amyrin, β-amyrin, lupeol, and their respective acetates, 3- O-acetyl derivatives of betulinic, oleanolic, and ursolic acids, cycloartenol, and tirucall-7,24-dienol), two new flavonols presenting an uncommon interglycosidic O-(1→3) linkage (kaempferol 3- O-α-L-arabinofuranosyl-(1→3)-α-L-rhamnoside and quercetin 3- O-α-L-arabinofuranosyl-(1→3)-α-L-rhamnoside), β-sitosterol, stigmasterol, quercetin, and gallic acid were isolated from the Amazonian medicinal mistletoe, Cladocolea micrantha Kuijt (Loranthaceae). Their structures were established by spectral methods and eventual chromatographic comparisons. The quercetin derivative was not cytotoxic to MV3 human melanoma cells, but was able, when administered at 1 μg/mL, to promote a twofold inhibition of the migration of the cells through the transwell system when compared with paclitaxel at 5 μM.

scholarly journals Antibodies to synthetic peptide from the residue 33 to 42 domain of c-Ha-ras p21 block reconstitution of the protein with different effectors.

1989 ◽  
Vol 9 (9) ◽  
pp. 3904-3910 ◽  
Author(s):  
I Rey ◽  
P Soubigou ◽  
L Debussche ◽  
C David ◽  
A Morgat ◽  
...  
Keyword(s):  
Synthetic Peptide ◽  
Binding Pocket ◽  
Inhibitory Effect ◽  
Effector Proteins ◽  
Structural Basis ◽  
Ras Proteins ◽  
Ras P21 ◽  
Reduced Rate ◽  
Additional Support

Residues 32 to 40, which are conserved among ras proteins from different species, are likely to participate in interactions with the p21 effector system. With the goal of understanding the structural basis of the regulatory functions of c-Ha-ras p21, we produced rabbit antisera against a synthetic peptide corresponding to amino acids 33 to 42 of the protein. The affinity-purified antibodies interacted specifically with p21 and with the antigenic peptide. The epitope recognized by the antibodies appeared to be centered on threonine 35. The antibodies inhibited both in vitro p21-induced production of cyclic AMP in detergent extracts of RAS-defective yeast membranes and GAP-stimulated GTPase activity. However, monoclonal anti-ras antibodies Y13-259 and Y13-238 were not capable of specifically inhibiting interactions of p21 with these two putative effector proteins. The apparent inhibitory effect of Y13-259 on stimulation of p21 by GAP was due to a greatly reduced rate of exchange of nucleotides in the binding pocket of the protein. These findings provide additional support for the essential role of the residue 32 to 40 domain as the true effector site and further evidence of the involvement of GAP as a cellular effector of ras proteins.

scholarly journals PAF104, a Synthetic Peptide to Control Rice Blast Disease by Blocking Appressorium Formation in Magnaporthe oryzae

2013 ◽  
Vol 26 (12) ◽  
pp. 1407-1416 ◽  
Author(s):  
Aarón Rebollar ◽  
Belén López-García
Keyword(s):  
Magnaporthe Oryzae ◽  
Synthetic Peptide ◽  
Rice Blast ◽  
Surface Protein ◽  
Inhibitory Effect ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Appressorium Formation ◽  
Hydrophobic Surfaces

Magnaporthe oryzae is the most devastating pathogen of rice and the main cause of crop losses worldwide. The successful management of blast disease caused by this fungus is a clear necessity. The synthetic peptide PAF104 has been characterized by its inhibition of M. oryzae appressorium formation on hydrophobic surfaces. Growth and the ability of conidia to germinate was not affected by PAF104, indicating the lack of toxicity on fungal conidia. The addition of the cutin monomer 1,16-hexadecanediol does not interfere with the inhibitory effect of PAF104 on in vitro hydrophobic surfaces. On the other hand, inhibition of appressorium formation by PAF104 was nullified by the exogenous addition of cAMP. Our results suggest that PAF104 affects the Pmk1 pathway by repression of the gene expression of MoMSB2, which encodes a sensing surface protein, and the mitogen-activated protein/extracellular signal-regulated kinase kinase kinase MST11. The pathogenicity of M. oryzae was reduced after PAF104 treatment specifically blocking appressorium formation. Our results support PAF104 as a promising compound to control rice blast disease by blocking a specific target related to appressorium formation, a process essential for infection of rice leaves. Moreover, PAF104 is proposed as a lead compound to develop novel specific fungicides with improved properties.

scholarly journals Effect of Alkyl Chain Length on Interfacial Performance of Alkyl Gallates in Supramolecular Oxidation of Soybean Bulk Phase Oil

2021 ◽  
Author(s):  
Mojtaba Delfanian ◽  
M. A. Sahari ◽  
Mohsen Barzegar ◽  
Hassan Ahmadi Gavlighi ◽  
Francisco Jose Barba
Keyword(s):  
Gallic Acid ◽  
Chain Length ◽  
Alkyl Chain ◽  
Inhibitory Effect ◽  
Bulk Phase ◽  
Log P ◽  
Bulk Oil ◽  
Alkyl Gallates ◽  
Critical Chain ◽  
Better Than

From an interfacial phenomena standpoint, the effect of length of alkyl chain was evaluated on activities of gallic acid and its alkyl ester derivatives in supramolecular oxidation of stripped soybean oil. The gallic acid molecules (log P = -0.47) with an electron-donating carboxylate anion had a higher scavenging activity against DPPH• than alkyl gallates. Interfacial activities of gallates in emulsion and bulk oil improved as the lengths of the alkyl chain were reached until a critical chain length; after that, further chain length extension causes a decrease in potency. In β-carotene bleaching and Rancimat assays higher inhibitory effect was observed for dodecyl and propyl gallate, respectively. Evaluation of interfacial tension, water content, and droplet size of hydroperoxides reverse micelles in parallel with lipid peroxidation indicated that the inhibitory effect of the alkyl gallates, especially methyl gallate, was significantly better than the gallic acid in the bulk phase oil.

scholarly journals Methyl gallate, gallic acid-derived compound, inhibit cell proliferation through increasing ROS production and apoptosis in hepatocellular carcinoma cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248521
Author(s):  
Chien-Yu Huang ◽  
Yu-Jia Chang ◽  
Po-Li Wei ◽  
Chin-Sheng Hung ◽  
Weu Wang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Western Blot ◽  
Gallic Acid ◽  
Annexin V ◽  
Inhibitory Effect ◽  
Ros Production ◽  
Methyl Gallate ◽  
Induced Apoptosis

Hepatocellular carcinoma (HCC) is a global health problem. Currently, there is no effective therapeutic strategy for HCC. Methyl gallate (MG), from plant-derived phenolic gallic acid, has exhibited antitumor efficacy. However, the effect of MG on HCC is unclear. In vitro growth activity was detected by a sulforhodamine assay. A zebrafish xenotransplantation was applied to evaluate the inhibitory effect of MG. Reactive oxygen species (ROS) production, autophagy, and lysosome formation were detected by specific dyes. Finally, apoptosis was examined using annexin V-FITC/PI staining and western blot was performed to determine the molecular mechanism. It was demonstrated that MG treatment inhibited the proliferation of Hep3B, Mahlavu, and HepJ5 cells. Xenotransplantation also showed that MG inhibited the growth of Hep3B and HepJ5 cells. MG treatment increased cellular levels of superoxide and oxidative stress. Increases in autophagy and lysosome formation were found after MG treatment. The western blot analysis showed that MG activated cleavage of caspase-3 and poly (SDP ribose) polymerase (PARP), modulated levels of the Bcl2, Bax, and Bad ligands, and induced apoptosis. MG induced autophagy with notable activation of beclin-1, autophagy related 5+12 (ATG5+12), and conversion of light chain 3-I (LC3-I) to II. Our study showed that MG exposure inhibited HCC proliferation both in vitro and in vivo. And blocking autophagy enhanced MG-induced cytotoxicity in HCC cells. These findings suggested MG might serve as a powerful therapeutic supplement for human HCC patients.

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