Isolation, characterization and biological activity of betulinic acid and ursolic acid from Vitex negundo L.

2003 ◽  
Vol 17 (2) ◽  
pp. 129-134 ◽  
Author(s):  
C. Chandramu ◽  
Rao D. Manohar ◽  
David G. L. Krupadanam ◽  
Reddy V. Dashavantha
Keyword(s):  
Biological Activity ◽  
Ursolic Acid ◽  
Betulinic Acid ◽  
Vitex Negundo

Synthesis and Biological Activity of 28-Amide Derivatives of 23-Hydroxy Betulinic Acid as Antitumor Agent Candidates

2013 ◽  
Vol 9 (7) ◽  
pp. 920-925 ◽  
Author(s):  
Yi Bi ◽  
Jinyi Xu ◽  
Fei Sun ◽  
Xiaoming Wu ◽  
Wencai Ye ◽  
...  
Keyword(s):  
Biological Activity ◽  
Betulinic Acid ◽  
Antitumor Agent ◽  
Amide Derivatives ◽  
Derivatives Of

scholarly journals 21,28-Epoxy-18β,21β-Dihydroxbaccharan-3-one and Other Terpenoids from the Liverwort Lepidozia Chordulifera T. Taylor

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301
Author(s):  
Hildegard Zapp ◽  
Kerstin Orth ◽  
Josef Zapp ◽  
Joseph D. Connolly ◽  
Hans Becker
Keyword(s):  
Ursolic Acid ◽  
Betulinic Acid ◽  
Spectroscopic Properties ◽  
Betulonic Acid ◽  
Side Chain

A new triterpenoid, 21,28-epoxy-18β,21β-dihydroxybaccharan-3-one (1), with a spiro-hemiketal side-chain, has been isolated from the liverwort Lepidozia chordulifera. The plant also yielded (6S*, 7S*)-3-norhimachal-1-en-3-one (3), an epimer of a known norhimachalane, the rare 3β-hydroxy-22,23,24,25,26,27-hexanordammaran-20-one and the triterpenoids shoreic acid, taraxerol, betulinic acid, betulonic acid and ursolic acid. Their structures have been assigned on the basis of their spectroscopic properties.

LC-MS Method for the Detection and Quantification of Ursolic Acid and Uvaol Levels in Olive Leaves and Oregano

2020 ◽  
pp. 600
Author(s):  
Naser F. Al-Tannak ◽  
Ladislav Novotny
Keyword(s):  
Biological Activity ◽  
Ursolic Acid ◽  
Mobile Phase ◽  
Solid Phase ◽  
Phase Extraction ◽  
Origanum Vulgare ◽  
Olive Leaves ◽  
Accuracy And Precision ◽  
Acquity Uplc ◽  
Detection And Quantification

Ursolic acid and uvaol belong to pentacyclic triterpenes are both secondary metabolites with significant biological activity. Ursolic acid and uvaol are exist in many natural matrices such as olive leaves, oregano and thymus. Therefore, a new rapid and validated UPLC method was developed to identify and quantify ursolic acid and uvaol in dry olive leaves (Olea europaea L.) planted in Kuwait and dry oregano (Origanum vulgare L.) planted in Palestine and Spain. The separation of ursolic acid and uvaol was achieved by using BEH Phenyl (1.7 μm, 2.1 x 100 mm) analytical column (Waters® Acquity UPLC) and a mobile phase composed of water and acetonitrile (37:63 v/v). Solid-phase extraction cartridges (HyperSep™) was used to extract ursolic acid and uvaol from natural matrices. Ursolic acid and uvaol were detected in all-natural matrices used. The concentrations of ursolic acid and uvaol in olive leaves were 55.10 μg/g ± 0.72 and 314.29 μg/g ± 0.97, respectively. In contrast, ursolic acid and uvaol concentrations in oregano brands were ranging from 25.57 μg/g ± 0.35- 28.70 μg/g ± 0.36 and 19.07 μg/g ± 0.38 - 48.10 μg/g ± 0.62, respectively. In conclusion, the developed method was capable to identify and determine ursolic acid and uvaol in pure powder and natural matrices with good linearity, accuracy and precision.

scholarly journals Characterization and Quantification of Triterpenes in the Neotropical Medicinal Plant Souroubea sympetala (Marcgraviaceae) by HPLC-APCI-MS

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Martha Mullally ◽  
Kari Kramp ◽  
Ammar Saleem ◽  
Marco Otorola Rojas ◽  
Pablo Sanchez Vindas ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Ursolic Acid ◽  
Betulinic Acid ◽  
Plant Organs ◽  
Immature Fruit ◽  
Pentacyclic Triterpenes ◽  
Wood Bark

A rapid, two-solvent, HPLC-APCI-MS method was developed to identify and quantify four pentacyclic triterpenes (betulinic acid, ursolic acid, α-amyrin and β-amyrin) in extracts of the neotropical medicinal plant Souroubea sympetala. Analysis of plant organs, wood, bark, leaves, immature fruit and flowers, indicated that the phytochemical distribution and quantity of triterpenes varies across the plant, with betulinic acid and ursolic acid the major constituents in the bark, wood, fruit and flowers and the amyrins the major constituents of the leaves.

scholarly journals Platelet Antiaggregating Activity and Chemical Constituents of Salvia x Jamensis J. Compton

2008 ◽  
Vol 3 (6) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Angela Bisio ◽  
Giovanni Romussi ◽  
Eleonora Russo ◽  
Nunziatina De Tommasi ◽  
Nicola Mascolo ◽  
...  
Keyword(s):  
Data Analysis ◽  
Platelet Aggregation ◽  
Ursolic Acid ◽  
Betulinic Acid ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Isopimaric Acid ◽  
Phytochemical Study ◽  
Rat Platelets ◽  
Significant Concentration

A phytochemical study has been carried out on the surface exudate of Salvia x jamensis, which showed a significant platelet antiaggregating activity. The known compounds isopimaric acid (2), 14-α-hydroxy-isopimaric acid (3), 3β-hydroxy-isopimaric acid (4), 7,8β-dihydrosalviacoccin (5), betulinic acid (6), and ursolic acid (7) were isolated together with the new diterpene 1. The structure of 1 was determined as 15,16-epoxy-cleroda-3-en-7α,10β-dihydroxy-12,17;19,18-diolide on the basis of spectroscopic data analysis. Among all tested compounds, 2 showed a significant concentration-dependent antiaggregating activity when ADP (3 μM) was used as agonist on rat platelets. Conversely, 1 increased ADP–induced platelet aggregation.

Lupane-Triterpene Esters from the Leaves of Ceriops decandra (Griff.) Ding Hou

2005 ◽  
Vol 58 (8) ◽  
pp. 615 ◽  
Author(s):  
Chanita Ponglimanont ◽  
Pakakrong Thongdeeying
Keyword(s):  
Ursolic Acid ◽  
Betulinic Acid ◽  
Spectroscopic Methods ◽  
Ceriops Decandra ◽  
New Compounds ◽  
First Time ◽  
Triterpene Esters

Two novel triterpene esters were isolated from the leaves of Ceriops decandra in addition to 16 known triterpenes: lupenone 3, lupeol 4, betulinaldehyde 5, 3β-Z-coumaroyllupeol 6, 3β-E-coumaroyllupeol 7, 3-epi-betulinic acid 8, betulin 9, betulinic acid 10, 3β-E-feruloylbetulin 11, 30-nor-lup-3β-ol-20-one 12, 3β-E-caffeoyllupeol 13, lup-20(29)-en-3β,30-diol 14, 3β-hydroxylupan-29-oic acid 15, 3β,20-dihydroxylupane 16, and a mixture of oleanolic and ursolic acid 17 and 18. The new compounds were determined by spectroscopic methods to be 3β-E-feruloyllupeol 1 and 3β-Z-feruloyllupeol 2. Compounds 3 and 5–16 were reported for the first time as metabolites of C. decandra.

scholarly journals In silico screening of W. salutaris bioactive constituents reveal betulinic acid, ursolic acid acetate and eucosterol as potential M. tuberculosis antagonists targeting FabF

2021 ◽  
Author(s):  
Nokukhanya Gumede ◽  
Kgothatso E. Machaba ◽  
Umar Ndagi ◽  
Hezekiel M. Kumalo ◽  
Ndumiso N. Mhlongo
Keyword(s):  
Ursolic Acid ◽  
Betulinic Acid ◽  
In Silico ◽  
Fatty Acid Biosynthesis ◽  
Binding Energies ◽  
Active Site Residues ◽  
Bioactive Constituents ◽  
Inhibitory Potential ◽  
Dynamics Simulations ◽  
Potential Inhibitors

Abstract Tuberculosis (TB) remains a long-standing burdening disease to control worldwide. The lengthy current TB treatment, which boasts with unbearable adverse effects, and frequent emergence of drug resistant strains of M. tuberculosis lays an increasing burden. This behests urgent discovery and development of alternative novel medicine to alleviate TB. In this report, in silico methods were applied to examine the propensity of W. salutaris active compounds as potential inhibitors of M. tuberculosis fatty acid biosynthesis protein (FabF). Thirteen compounds were virtually screened against FabF and subjected to molecular dynamics simulations and post-dynamics analyses to examine their inhibitory potential. Betulinic acid, ursolic acid and ursolic acid acetate had the best binding energies and hence the best inhibitory potential against FabF and desirable cytotoxicity profile. These compounds bind and interact with FabF active site residues to exert their inhibitory potential. Findings in this preliminary report warrant further experimental validation towards the development of these compounds as potential drugs targeting FabF in the treatment of tuberculosis.

scholarly journals Phytochemical Investigation and Antioxidative Capacity of Triterpenes Present in Plant Species Belonging to Lamiaceae Family

2020 ◽  
Keyword(s):  
Ursolic Acid ◽  
Betulinic Acid ◽  
Analytical Determination ◽  
Antioxidative Capacity ◽  
Dpph Method ◽  
Wide Range ◽  
Ic50 Values ◽  
Hexane Extracts ◽  
Dpph Scavenging ◽  
Rosmarinus Officinalis L

Triterpenes are persistently associated with observed bioactivities of extracts obtained from plant material that contains these very important natural products. Many species belonging to Lamiaceae family have been used for the presence of essential oil and very little is known about the presence of the triterpene substances in this family. Qualitative and quantitative analyses of this very important substances, in the aerial parts of eight species, all belonging to Lamiaceae family, were investigated in this study. Different extracts containing triterpene substances were tested by DPPH method to evaluate their antioxidative capacity. TLC and HPLC methods, used for the analytical determination of triterpenes, showed the presence of betulin, betulinic acid, ursolic acid and oleanolic acid. Betulin (3.2 mg/g) and betulinic acid (37.1 mg/g) were the most abundant triterpene components in the hexane extracts of Rosmarinus officinalis L. Ursolic acid (0.14 mg/g) was the most abundant triterpene compound in the hexane extract of Thymus pulegioides L. All tested samples demonstrated DPPH scavenging activity in a concentration dependant manner, with a wide range of IC50 values from 0.4 mg/mL to 3.3 mg/mL.

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