Chemistry and Functionality of Cold-Pressed Macadamia Nut Oil

The rising trend in the consumption of healthy, safe, and functional foods has motivated studies on cold-pressed specialty oils, including macadamia nut oil. Cold-pressed macadamia nut oil (CPMO) is given preference by consumers over solvent extracted and refined oil because of its exceptional quality attributes and safety. This review contains a detailed presentation of the chemical properties, health benefits, and applications of CPMO. The monounsaturated fatty acids (oleic acid and palmitoleic acid) rich oil also contains a significant concentration of bioactive phytochemicals including, β-sitosterol, α-tocopherol, α-tocotrienols, ρ-hydroxybenzoic acid, and caffeic acid. Moreover, the oil has good oxidative stability. The highlighted properties offer CPMO health benefits related to the prevention of cardiovascular diseases, diabetes, cancer, high blood pressure, and neurodegenerative diseases. The fatty acid composition of CPMO allows for its diverse application in the food, cosmetic, nutraceutical, and pharmaceutical industries.

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Bergapten, a furocoumarin found in many medicinal plants, is used for the management of various conditions. The present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation. Bergapten administered at 10, 30, and 100 μg/ml exhibited a significant concentration-dependent protection on the erythrocyte membrane exposed to hypotonicity and heat-induced hemolysis. The concentration at which bergapten inhibited 50% of the cells from hemolysis (IC50) was determined on a dose-response curve, plotted as logarithmic (concentration) against percentage inhibition, keeping the hemolysis produced within the control group at 100%. Bergapten treatment produced an IC50 value of 7.71 ± 0.27 μg/ml and 4.23 ± 0.42 μg/ml for hypotonicity and heat-induced hemolysis, respectively. Diclofenac sodium at similar concentrations produced an IC50 value of 12.22 ± 0.30 μg/ml and 9.44 ± 0.23 μg/ml in the hypotonicity and heat-induced hemolysis, respectively. The ability of bergapten to inhibit protein denaturation was studied as part of an investigation on its mechanism of action. The results showed a significant concentration-dependent reduction in protein denaturation. When administered at 10, 30, and 100 μg/ml, bergapten produced a concentration-dependent reduction in albumin denaturation. Bergapten inhibited protein denaturation with IC50 values of 5.34 ± 0.30 μg/ml and 12.18 ± 0.20 μg/ml in the heat-treated egg albumin and bovine serum albumin denaturation experiments, respectively. Diclofenac sodium (10, 30, and 100 μg/ml) exhibited a similar protection against heat-treated egg albumin and bovine serum albumin denaturation experiments with IC50 values of 8.93 ± 0.17 μg/ml and 12.72 ± 0.11 μg/ml, respectively. Taken together, data from this study show that the pharmacological properties of bergapten may in part be related to its membrane-stabilizing and antidenaturation properties.

Fresh and pasteurized orange juice analysis by TXRF

Orange is considered the main product of the Brazilian citrus agro-industrial complex. However, in the end of 2016, the ANVISA pointed out orange as a risky product due to contamination using pesticides in its cultivation. Therefore, in this context, an analysis of the chemical elements present in fresh and pasteurized orange juices becomes extremely relevant. Thus, this work aims to quantify the elements that are present in fresh and pasteurized orange juices, using the TXRF technique. Samples of fresh orange juice of Pêra variety were acquired in a store in the city of Londrina-PR, were analysed; three samples of oranges were purchased at a store in Itápolis, São Paulo and five more samples of Pêra orange were obtained in the rural area. Samples of pesticides used in orange cultivation were quantified, and three different trademarks of pasteurized juice were analysed. In some of the samples that were collected in Itápolis-SP market, lead (Pb) element was quantified, however its concentration was within the limit established by Brazilian legislation. The aluminium (Al) element was detected in all samples of pasteurized orange juice, showing the influence of the packaging on the elemental concentration of the juice. All pesticides quantified in this work showed a significant concentration of some micro-contaminants, but when the pesticide was diluted in deionized water, those micro-contaminants could not be quantified.

Nicht nur als Kopfschmuck getragen: ein außergewöhnlicher hallstattzeitlicher Fund aus Smržice-Trávníky u ostrova (Mittelmähren)

This study deals with the cultural and spatial analysis of finds discovered in the previous five years during surface prospecting in Smržice-Trávníky u ostrova (Prostějov district) on the area of a presumed settlement of the Platěnice group of the East Hallstatt culture. In the set of the analysed items, in addition to local ceramics of the Platěnice group and several metal objects of the Hallstatt type (rare type of a bronze boat-shaped fibula, a bronze pin with a hemispherical head and a ribbed neck, a bronze conical arrowhead of circular cross-section) dating to Ha C2 and Ha D1, there were also bronze arrowheads of the eastern type (with an inner socket), which demonstrate the connections between the Prostějov region and the Vekerzug culture during the Ha D period. An exceptional find of a ceramic fragment of a local (Platěnice group) vessel decorated with impressions of small nail-shaped earrings probably from Ha D1 indicates, on the contrary, the contacts of Central Moravia with the late phase of the Tarnobrzeg Lusatian culture of Southeast Poland. It is the Prostějov region, where we observe a significant concentration of objects of the eastern type during the Hallstatt period. Therefore, special attention in the study was paid to Hallstatt period finds of the eastern type with the focus on the provenience, chronological aspect and interpretation of their occurrence in Moravia as well as in the neighboring regions.

Environmentally Relevant Concentrations of Triclosan Induce Cyto-Genotoxicity and Biochemical Alterations in the Hatchlings of Labeo rohita

Xenobiotic Triclosan (TCS) is of great concern because of its existence in a variety of personal, household and healthcare products and continuous discharge in water worldwide. Excessive use of TCS-containing sanitizers and antiseptic products during the COVID-19 pandemic further increased its content in aquatic ecosystems. The present study deals with the cyto-genotoxic effects and biochemical alterations in the hatchlings of Labeo rohita on exposure to environmentally relevant concentrations of TCS. Three-days-old hatchlings were exposed to tap water, acetone (solvent control) and 4 environmentally relevant concentrations (6.3, 12.6, 25.2 and 60 µg/L) of TCS for 14 days and kept for a recovery period of 10 days. The significant concentration-dependent decline in cell viability but increase in micronucleated cells, nucleo-cellular abnormalities (NCAs) and DNA damage parameters like tail length, tail moment, olive tail moment and percent of tail DNA after exposure persisted till the end of recovery period. Glucose, triglycerides, cholesterol, total protein, albumin, total bilirubin, uric acid and urea (except for an increase at 60 µg/L) showed significant (p ≤ 0.05) concentration-dependent decrease after 14 days of exposure. The same trend (except for triglycerides, albumin and total bilirubin) continued till 10 days post exposure. In comparison to control, transaminases (alanine and aspartate aminotransferases) increased (p ≤ 0.05) after exposure as well as the recovery period, while a decline in alkaline phosphatase after exposure was followed by a significant increase during the recovery period. The results show that the environmentally relevant concentrations of TCS cause deleterious effects on the hatchlings of L. rohita.

Scaling Down Large-Scale Thawing of Monoclonal Antibody Solutions: 3D Temperature Profiles, Changes in Concentration, and Density Gradients

Purpose Scale-down devices (SDD) are designed to simulate large-scale thawing of protein drug substance, but require only a fraction of the material. To evaluate the performance of a new SDD that aims to predict thawing in large-scale 2 L bottles, we characterised 3D temperature profiles and changes in concentration and density in comparison to 125 mL and 2 L bottles. Differences in diffusion between a monoclonal antibody (mAb) and histidine buffer after thawing were examined. Methods Temperature profiles at six distinct positions were recorded with type T thermocouples. Size-exclusion chromatography allowed quantification of mAb and histidine. Polysorbate 80 was quantified using a fluorescent dye assay. In addition, the solution’s density at different locations in bottles and the SDD was identified. Results The temperature profiles in the SDD and the large-scale 2 L bottle during thawing were similar. Significant concentration gradients were detected in the 2 L bottle leading to marked density gradients. The SDD slightly overestimated the dilution in the top region and the maximum concentrations at the bottom. Fast diffusion resulted in rapid equilibration of histidine. Conclusion The innovative SDD allows a realistic characterisation and helps to understand thawing processes of mAb solutions in large-scale 2 L bottles. Only a fraction of material is needed to gain insights into the thawing behaviour that is associated with several possible detrimental limitations.

Ultramicronized Palmitoylethanolamide Inhibits NLRP3 Inflammasome Expression and Pro-Inflammatory Response Activated by SARS-CoV-2 Spike Protein in Cultured Murine Alveolar Macrophages

Despite its possible therapeutic potential against COVID-19, the exact mechanism(s) by which palmitoylethanolamide (PEA) exerts its beneficial activity is still unclear. PEA has demonstrated analgesic, anti-allergic, and anti-inflammatory activities. Most of the anti-inflammatory properties of PEA arise from its ability to antagonize nuclear factor-κB (NF-κB) signalling pathway via the selective activation of the PPARα receptors. Acting at this site, PEA can downstream several genes involved in the inflammatory response, including cytokines (TNF-α, Il-1β) and other signal mediators, such as inducible nitric oxide synthase (iNOS) and COX2. To shed light on this, we tested the anti-inflammatory and immunomodulatory activity of ultramicronized(um)-PEA, both alone and in the presence of specific peroxisome proliferator-activated receptor alpha (PPAR-α) antagonist MK886, in primary cultures of murine alveolar macrophages exposed to SARS-CoV-2 spike glycoprotein (SP). SP challenge caused a significant concentration-dependent increase in proinflammatory markers (TLR4, p-p38 MAPK, NF-κB) paralleled to a marked upregulation of inflammasome-dependent inflammatory pathways (NLRP3, Caspase-1) with IL-6, IL-1β, TNF-α over-release, compared to vehicle group. We also observed a significant concentration-dependent increase in ACE-2 following SP challenge. um-PEA concentration-dependently reduced all the analyzed proinflammatory markers fostering a parallel downregulation of ACE-2. Our data show for the first time that um-PEA, via PPAR-α, markedly inhibits the SP induced NLRP3 signalling pathway outlining a novel mechanism of action of this lipid against COVID-19.

Phosphorus recovery by struvite precipitation: a review of the impact of calcium on struvite quality

Precipitation of phosphorus (P) as a struvite product from wastewater and local magnesium (Mg) sources is considered as a high economical prudent venture, as it has shown to bring down the cost of producing the product. However, there is a significant concentration of calcium (Ca) in these Mg and most wastewater sources. The presence of Ca in wastewater and Mg sources during struvite precipitation has been shown to negatively affect the quality of the struvite produced. The low struvite quality greatly impedes its use as a fertiliser given the economic value of the product as an alternative P fertiliser. This review paper provides an understanding of how the presence of Ca affects the purity, crystal size, and morphology of the struvite. Different methods that have been used to mitigate the effect of Ca on struvite quality have been reviewed. The factors that affect the recovery of P and the percentage recoveries of P by different Mg sources have also been reviewed. This paper serves as a basis for further research where the possibility of removing Ca from wastewater and Mg sources is explored. When Ca is removed before struvite precipitation is carried out, the quality of the product will be greatly improved.

Red Algal Sulfated Galactan Binds and Protects Neural Cells from HIV-1 gp120 and Tat

The potential neuroprotective capacity of four different sulfated glycans: Botryocladia occidentalis-derived sulfated galactan (BoSG) (MW > 100 kDa), Lytechinus variegatus-derived sulfated fucan (LvSF) (MW~90 kDa), high-molecular weight dextran sulfate (DxS) (MW 100 kDa), and unfractionated heparin (UFH) (MW~15 kDa), was assessed in response to the HIV-1 proteins, R5-tropic glycoprotein 120 (gp120) and/or trans-activator of transcription (Tat), using primary murine neurons co-cultured with mixed glia. Compared to control-treated cells in which HIV-1 proteins alone or combined were neurotoxic, BoSG was, among the four tested sulfated glycans, the only one capable of showing significant concentration-dependent neuroprotection against Tat and/or gp120, alone or combined. Surface plasmon resonance-based data indicate that BoSG can bind both HIV-1 proteins at nM concentrations with preference for Tat (7.5 × 10−8 M) over gp120 (3.2 × 10−7 M) as compared to UFH, which bound gp120 (8.7 × 10−7 M) over Tat (5.7 × 10−6 M). Overall, these data support the notion that sulfated glycan extracted from the red alga B. occidentalis, BoSG, can exert neuroprotection against HIV-1 Tat and gp120, potentially via direct molecular interactions.

Aluminum-Free Steelmaking: Desulfurization and Nonmetallic Inclusion Evolution of Si-Killed Steel in Contact with CaO-SiO2-CaF2-MgO Slag

In some applications, deep desulfurization and deoxidation of steels without the use of aluminum are required, using Si as a deoxidant instead, with double-saturated slags in the CaO-SiO2-CaF2-MgO system. This work studied the desulfurization and nonmetallic inclusion evolution for the system using an induction furnace and compared the results with FactSage kinetic simulations. Steel samples were taken from the steel melt and analyzed with ICP-MS and combustion analysis for chemistry, and SEM/EDS for nonmetallic inclusion quantity, size, and composition. The results indicate that the steel was deeply desulfurized, with a final sulfur partition coefficient of 580; MgO was reduced from the slag, yielding dissolved [Mg] that transformed liquid Mn–silicate inclusions into forsterite and MgO. Intentional reoxidation of the melt with oxidized electrolytic iron demonstrated a significant concentration of dissolved [Mg] in the steel, by the formation of additional forsterite and MgO upon reoxidation.