scholarly journals Biomaterials: Efficient Encapsulation of Fe3O4Nanoparticles into Genetically Engineered Hepatitis B Core Virus-Like Particles Through a Specific Interaction for Potential Bioapplications (Small 9-10/2015)

Small ◽  
2015 ◽  
Vol 11 (9-10) ◽  
pp. 1189-1189
Author(s):  
Lihua Shen ◽  
Jun Zhou ◽  
Yixiao Wang ◽  
Ning Kang ◽  
Xuebin Ke ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Specific Interaction ◽  
Genetically Engineered ◽  
Virus Like Particles

scholarly journals Hepatitis B core-based virus-like particles: A platform for vaccine development in plants

2021 ◽  
Vol 29 ◽  
pp. e00605
Author(s):  
Maryam Moradi Vahdat ◽  
Farshad Hemmati ◽  
Abozar Ghorbani ◽  
Daria Rutkowska ◽  
Alireza Afsharifar ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Vaccine Development ◽  
Virus Like Particles

scholarly journals Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Gergana Zahmanova ◽  
Milena Mazalovska ◽  
Katerina Takova ◽  
Valentina Toneva ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nicotiana Benthamiana ◽  
Hepatitis E Virus ◽  
Chimeric Protein ◽  
Hepatitis E ◽  
Core Antigen ◽  
Efficient Production ◽  
Virus Like Particles ◽  
B Virus

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

scholarly journals Virus-like Particles‑Application in Nano Vaccines: A Review

2020 ◽  
Vol 10 (03) ◽  
pp. 366-368
Author(s):  
Ghassaq Tariq Alubaidi
Keyword(s):  
Control Release ◽  
Stability Control ◽  
Genetically Engineered ◽  
Structural Units ◽  
Target Delivery ◽  
Virus Like Particles ◽  
Nano Size ◽  
New Strategies ◽  
New Vaccines

Nanomaterials are increasingly applied to develop new vaccines with new strategies. Implementation of such substances in vaccines will enhance vaccine formula immunogenicity, target delivery, and antigen stability control release. Genetically engineered virus-like particles (VLPs), structurally mimic the viruses and had been successfully used as nano vaccines. VLPs-based vaccines possess the advantage of being safe, effective, and non-infectious. Moreover, due to the optimized nano-size and repetitive structural units of the VLPs, it is suspected that those particles are highly immunogenic, even in absence of adjuvant substances. VLPs could be formulated to carry an array of heterogeneous antigens of different viruses. For all, they are considered as ideal nano vaccine model.

Dual-Antigen-Loaded Hepatitis B Virus Core Antigen Virus-like Particles Stimulate Efficient Immunotherapy Against Melanoma

2020 ◽  
Vol 12 (48) ◽  
pp. 53682-53690
Author(s):  
Keman Cheng ◽  
Tao Du ◽  
Yao Li ◽  
Yingqiu Qi ◽  
Huan Min ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Antigen ◽  
Virus Like Particles ◽  
Virus Core ◽  
B Virus

Hepatitis B virus-like particles expressing Plasmodium falciparum epitopes induce complement-fixing antibodies against the circumsporozoite protein

Vaccine ◽  
2019 ◽  
Vol 37 (12) ◽  
pp. 1674-1684 ◽  
Author(s):  
Natalie J. Kingston ◽  
Liriye Kurtovic ◽  
Renae Walsh ◽  
Carina Joe ◽  
George Lovrecz ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Circumsporozoite Protein ◽  
Virus Like Particles ◽  
B Virus

scholarly journals Phase I Testing of a Malaria Vaccine Composed of Hepatitis B Virus Core Particles Expressing Plasmodium falciparum Circumsporozoite Epitopes

2004 ◽  
Vol 72 (11) ◽  
pp. 6519-6527 ◽  
Author(s):  
Elizabeth H. Nardin ◽  
Giane A. Oliveira ◽  
J. Mauricio Calvo-Calle ◽  
Kristiane Wetzel ◽  
Carolin Maier ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Phase I ◽  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Virus Like Particles ◽  
Virus Core ◽  
B Virus ◽  
Malaria Vaccine Candidate

ABSTRACT We report the first phase I trial to assess the safety and immunogenicity of a malaria vaccine candidate, ICC-1132 (Malarivax), composed of a modified hepatitis B virus core protein (HBc) containing minimal epitopes of the Plasmodium falciparum circumsporozoite (CS) protein. When expressed in Escherichia coli, the recombinant ICC-1132 protein forms virus-like particles that were found to be highly immunogenic in preclinical studies of mice and monkeys. Twenty healthy adult volunteers received a 20- or a 50-μg dose of alum-adsorbed ICC-1132 administered intramuscularly at 0, 2, and 6 months. The majority of volunteers in the group receiving the 50-μg dose developed antibodies to CS repeats as well as to HBc. Malaria-specific T cells that secreted gamma interferon were also detected after a single immunization with ICC-1132-alum. These studies support ICC-1132 as a promising malaria vaccine candidate for further clinical testing using more-potent adjuvant formulations and confirm the potential of modified HBc virus-like particles as a delivery platform for vaccines against other human pathogens.

Sign in / Sign up

Export Citation Format

Share Document