A Microfluidic Device to Fabricate One‐Step Cell Bead‐Laden Hydrogel Struts for Tissue Engineering (Small 1/2022)

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

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One-Step Production Using a Microfluidic Device of Highly Biocompatible Size-Controlled Noncationic Exosome-like Nanoparticles for RNA Delivery

ACS Applied Bio Materials ◽

10.1021/acsabm.0c01519 ◽

2021 ◽

Vol 4 (2) ◽

pp. 1783-1793

Author(s):

Niko Kimura ◽

Masatoshi Maeki ◽

Akihiko Ishida ◽

Hirofumi Tani ◽

Manabu Tokeshi

Keyword(s):

Microfluidic Device ◽

One Step ◽

Size Controlled

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Real-Time Detection of Escherichia coli in Water Pipe Using a Microfluidic Device with One-Step Latex Immunoagglutination Assay

Transactions of the ASABE ◽

10.13031/2013.27372 ◽

2009 ◽

Vol 52 (3) ◽

pp. 1031-1039

Author(s):

J.-Y. Yoon ◽

J.-H. Han ◽

C. Y. Choi ◽

M. Bui ◽

R. G. Sinclair

Keyword(s):

Escherichia Coli ◽

Real Time ◽

Microfluidic Device ◽

Water Pipe ◽

One Step ◽

Real Time Detection

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A multi-layer PDMS microfluidic device for tissue engineering applications

The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE ◽

10.1109/memsys.2003.1189774 ◽

2003 ◽

Cited By ~ 6

Author(s):

E. Leclerc ◽

Y. Sakai ◽

T. Fujii

Keyword(s):

Tissue Engineering ◽

Microfluidic Device ◽

Engineering Applications

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Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering

Micromachines ◽

10.3390/mi11070679 ◽

2020 ◽

Vol 11 (7) ◽

pp. 679

Author(s):

Uran Watanabe ◽

Shinji Sugiura ◽

Masayuki Kakehata ◽

Fumiki Yanagawa ◽

Toshiyuki Takagi ◽

...

Keyword(s):

Tissue Engineering ◽

Blood Vessel ◽

Microfluidic Device ◽

Vascular Function ◽

Laser Scanning ◽

Crosslinking Reaction ◽

Excitation Process ◽

Hollow Structures ◽

Photon Excitation

Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in general, it is difficult to fabricate hollow structures with a designed size and structure with a tens of micrometers scale for blood vessel tissue engineering. This study reports a method to fabricate the hollow structures in photodegradable hydrogels prepared in a microfluidic device. An infrared femtosecond pulsed laser, employed to induce photodegradation via multi-photon excitation, was scanned in the hydrogel in a program-controlled manner for fabricating the designed hollow structures. The photodegradable hydrogel was prepared by a crosslinking reaction between an azide-modified gelatin solution and a dibenzocyclooctyl-terminated photocleavable tetra-arm polyethylene glycol crosslinker solution. After assessing the composition of the photodegradable hydrogel in terms of swelling and cell adhesion, the hydrogel prepared in the microfluidic device was processed by laser scanning to fabricate linear and branched hollow structures present in it. We introduced a microsphere suspension into the fabricated structure in photodegradable hydrogels, and confirmed the fabrication of perfusable hollow structures of designed patterns via the multi-photon excitation process.

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One-step synthesis of chitosan-silica hybrid microspheres in a microfluidic device

Biomedical Microdevices ◽

10.1007/s10544-010-9463-9 ◽

2010 ◽

Vol 12 (6) ◽

pp. 1087-1095 ◽

Cited By ~ 30

Author(s):

Wenjie Lan ◽

Shaowei Li ◽

Jianhong Xu ◽

Guangsheng Luo

Keyword(s):

Microfluidic Device ◽

One Step ◽

Silica Hybrid

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Microfluidic Based Fabrication and Characterization of Highly Porous Polymeric Microspheres

Polymers ◽

10.3390/polym11030419 ◽

2019 ◽

Vol 11 (3) ◽

pp. 419 ◽

Cited By ~ 15

Author(s):

Benzion Amoyav ◽

Ofra Benny

Keyword(s):

Tissue Engineering ◽

Microfluidic Device ◽

Polymer Concentration ◽

Double Emulsion ◽

Polymeric Microspheres ◽

Porous Particles ◽

Floating Drug Delivery ◽

Porous Beads ◽

Batch Formulation ◽

New Applications

Polymeric porous particles are currently used for various applications in biotechnology, tissue engineering and pharmaceutical science, e.g., floating drug delivery systems and inhaled formulations. Particle shape and size depend on variable parameters; among them, polymer type and concentration, stirring speed, pH and type of solvent. In this study, porous poly(lactic-co-glycolic) acid (PLGA) and poly(d,l-lactide) (PLA) microspheres (MPs), with varying sizes and morphologies, were synthesized and optimized using both batch formulation and a flow-focusing microfluidic device. A well-established method of preparation utilizing solvent evaporation and the double emulsion technique was performed. Similar to other batch encapsulation methods, this technique is time and reagent consuming and consists of several steps. Hence, although porous structures provide tremendous opportunity in the design of new applications for tissue engineering and as improved controlled-release carriers, the synthesis of these particles with predefined properties remains challenging. We demonstrated the fabrication of porous MPs using a simple microfluidic device, compared to batch synthesis fabrication; and the effect of solvent, polymer concentration and type, post-hydrolysis treatment, on porosity degree. Moreover, a kinetic release study of fluorescent molecule was conducted for non-porous in comparison to porous particles. An overview of future prospects and the potential of these porous beads in this scientific area are discussed.

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