scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Hydrogel Properties and Their Impact on Regenerative Medicine and Tissue Engineering

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5795
Author(s):  
Adam Chyzy ◽  
Marta E. Plonska-Brzezinska
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Physicochemical Properties ◽  
Three Dimensional ◽  
Modern Medicine ◽  
Wound Treatment ◽  
Chemical Modifications

Hydrogels (HGs), as three-dimensional structures, are widely used in modern medicine, including regenerative medicine. The use of HGs in wound treatment and tissue engineering is a rapidly developing sector of medicine. The unique properties of HGs allow researchers to easily modify them to maximize their potential. Herein, we describe the physicochemical properties of HGs, which determine their subsequent applications in regenerative medicine and tissue engineering. Examples of chemical modifications of HGs and their applications are described based on the latest scientific reports.

Application of Microbial Polyesters-Polyhydroxyalkanoates as Tissue Engineering Materials

2005 ◽  
Vol 288-289 ◽  
pp. 437-440 ◽  
Author(s):  
Guo Qiang Chen ◽  
Qiong Wu ◽  
Ya Wu Wang ◽  
Zhong Zheng
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Surface Treatment ◽  
Surface Properties ◽  
Three Dimensional ◽  
Material Surface ◽  
New Class ◽  
Molecular Studies ◽  
Cartilage Cells ◽  
Engineering Materials

Poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) has improved mechanical properties over the existing PHA and our results have shown that PHBHHx has better biocompatibility over polyhydroxybutyrate (PHB) and polylactic acid (PLA). Surface treatment with lipases dramatically changed the material surface properties and increased the biocompatibility of the PHBHHx. PHBHHx and its PHB blends had been used to make three dimensional structures and it has been found that cartilage, osteoblast, and fibroblasts all showed strong growth on the PHBHHx scaffolds. The growth was much better compared with PLA. The molecular studies also showed that mRNA encoding cartilages were strongly expressed when cartilage cells were grown on the PHBHHx. As PHBHHx has strong mechanical properties, easily processible and biodegradable, this material can be used to develop a new class of tissue engineering materials.

scholarly journals Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

2019 ◽  
Vol 9 (17) ◽  
pp. 3540 ◽  
Author(s):  
Ferdows Afghah ◽  
Caner Dikyol ◽  
Mine Altunbek ◽  
Bahattin Koc
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Future Perspective ◽  
Melt Electrospinning ◽  
Wide Range ◽  
Challenges And Opportunities ◽  
Potential Applications ◽  
Homogeneous Cell

Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

Modification of Thai Silk Fibroin Scaffolds by Gelatin Conjugation for Tissue Engineering

2008 ◽  
Vol 55-57 ◽  
pp. 685-688 ◽  
Author(s):  
J. Chamchongkaset ◽  
Sorada Kanokpanont ◽  
David L. Kaplan ◽  
Siriporn Damrongsakkul
Keyword(s):  
Tissue Engineering ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Textile Industry ◽  
Three Dimensional ◽  
Biological Properties ◽  
Salt Leaching ◽  
Biological Compatibility ◽  
Salt Crystals

Silk has been used commercially as biomedical sutures for decades. Recently silk fibroin, especially from Bombyx mori silkworm, has been explored for many tissue engineering applications such as bone and cartilage due to its impressive biological compatibility and mechanical properties. In Thailand, Thai native silkworms have been long cultivated. Distinct characteristics of cocoon Thai silk are its yellow color and coarse filament. There is more sericin in Thai silk than in other Bombyx mori silks. These characteristics provide Thai silk a unique texture for textile industry. It is therefore the aim of this study to develop three-dimensional silk fibroin-based scaffolds from Thai yellow cocoon “Nangnoi-Srisaket” of Bombyx mori silkworms using salt-leaching method. To enhance the biological properties, type A gelatin, the denature form of collagen having good biocompactibility, was used to conjugate with silk fibroin scaffolds. The pore size of salt-leached silk fibroin scaffold structure represented the size of salt crystals used (600-710µm). After gelatin conjugation, gelatin was partly formed fibers inside the pores of silk fibroin scaffolds resulting in fiber-like structure with highly interconnection. Gelatin conjugation enhanced the compressive modulus of silk fibroin scaffolds by 93%. The results on in vitro culture using mouse osteoblast-like cells (MC3T3-E1) showed that gelatin conjugation could promote the cell proliferation in silk fibroin scaffolds. Moreover, the observed morphology of cells proliferated inside the scaffold after 14 days of culture showed the larger spreading area of cells on conjugated gelatin/silk fibroin scaffolds, compared to round-shaped cells on silk fibroin scaffolds. The results implied that Thai silk fibroin looked promising to be applied in tissue engineering and gelatin conjugation on Thai silk fibroin scaffolds could enhance the biological properties of scaffolds.

Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering

2015 ◽  
Vol 7 (1) ◽  
pp. 53-63 ◽  
Author(s):  
Nandana Bhardwaj ◽  
Wan Ting Sow ◽  
Dipali Devi ◽  
Kee Woei Ng ◽  
Biman B. Mandal ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Dermal Substitute ◽  
Skin Substitutes ◽  
3D Scaffolds ◽  
Skin Tissue Engineering ◽  
Dermal Tissue ◽  
Engineered Skin Substitutes

Development of highly vascular dermal tissue-engineered skin substitutes with appropriate mechanical properties and cellular cues is in need for significant advancement in the field of dermal reconstruction.

Combined Effects of Scaffold Material and Mechanical Stimulation on the Formation of Tissue Engineered Constructs Using Tendon and Ligament Progenitor Cells

2013 ◽  
Author(s):  
Andrew P. Breidenbach ◽  
Nathaniel A. Dyment ◽  
Yinhui Lu ◽  
Jason T. Shearn ◽  
David W. Rowe ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Progenitor Cells ◽  
Three Dimensional ◽  
Musculoskeletal Injuries ◽  
Combined Effects ◽  
Joint Movement ◽  
Scaffold Material ◽  
Ligament Injuries ◽  
Promising Alternative

Tendon and ligament injuries account for one-third of all musculoskeletal injuries [1]. Collagen fibrils in these mechanosensitive tissues transmit forces to mobilize and stabilize joint movement. Donor tissues used to repair these tissues often lack the mechanical properties of the tissue they are replacing. One promising alternative using tissue engineering combines stem/progenitor cells in three-dimensional tissue engineered constructs (TECs).

scholarly journals Alginate/Gelatin Hydrogels Reinforced with TiO2 and β-TCP Fabricated by Microextrusion-based Printing for Tissue Regeneration

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 457 ◽  
Author(s):  
Rodrigo Urruela-Barrios ◽  
Erick Ramírez-Cedillo ◽  
A. Díaz de León ◽  
Alejandro Alvarez ◽  
Wendy Ortega-Lara
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Average Pore Size ◽  
Three Dimensional ◽  
Engineering Applications ◽  
Average Pore ◽  
Viscous Deformation ◽  
Freeze Dried ◽  
3D Printed

Three-dimensional (3D) printing technologies have become an attractive manufacturing process to fabricate scaffolds in tissue engineering. Recent research has focused on the fabrication of alginate complex shaped structures that closely mimic biological organs or tissues. Alginates can be effectively manufactured into porous three-dimensional networks for tissue engineering applications. However, the structure, mechanical properties, and shape fidelity of 3D-printed alginate hydrogels used for preparing tissue-engineered scaffolds is difficult to control. In this work, the use of alginate/gelatin hydrogels reinforced with TiO2 and β-tricalcium phosphate was studied to tailor the mechanical properties of 3D-printed hydrogels. The hydrogels reinforced with TiO2 and β-TCP showed enhanced mechanical properties up to 20 MPa of elastic modulus. Furthermore, the pores of the crosslinked printed structures were measured with an average pore size of 200 μm. Additionally, it was found that as more layers of the design were printed, there was an increase of the line width of the bottom layers due to its viscous deformation. Shrinkage of the design when the hydrogel is crosslinked and freeze dried was also measured and found to be up to 27% from the printed design. Overall, the proposed approach enabled fabrication of 3D-printed alginate scaffolds with adequate physical properties for tissue engineering applications.

scholarly journals Electrospun nanofibers for the fabrication of engineered vascular grafts

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Sonia Fathi Karkan ◽  
Soodabeh Davaran ◽  
Reza Rahbarghazi ◽  
Roya Salehi ◽  
Abolfazl Akbarzadeh
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Physicochemical Properties ◽  
Review Paper ◽  
Vascular Grafts ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Electrospun Fibers

Abstract Attention has recently increased in the application of electrospun fibers because of their putative capability to create nanoscale platforms toward tissue engineering. To some extent, electrospun fibers are applicable to the extracellular matrix by providing a three-dimensional microenvironment in which cells could easily acquire definite functional shape and maintain the cell-to-cell connection. It is noteworthy to declare that placement in different electrospun substrates with appropriate physicochemical properties enables cells to promote their bioactivities, dynamics growth and differentiation, leading to suitable restorative effects. This review paper aims to highlight the application of biomaterials in engineered vascular grafts by using electrospun nanofibers to promote angiogenesis and neovascularization

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