scholarly journals Concise Review: Tissue-Specific Microvascular Endothelial Cells Derived From Human Pluripotent Stem Cells

Stem Cells ◽  
2014 ◽  
Vol 32 (12) ◽  
pp. 3037-3045 ◽  
Author(s):  
Hannah K. Wilson ◽  
Scott G. Canfield ◽  
Eric V. Shusta ◽  
Sean P. Palecek
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Microvascular Endothelial Cells ◽  
Tissue Specific ◽  
Concise Review ◽  
Microvascular Endothelial

scholarly journals Differentiation of Urine-Derived Induced Pluripotent Stem Cells to Neuron, Astrocyte and Microvascular Endothelial Cells from a Diabetic Patient

2019 ◽  
Author(s):  
Wan Liu ◽  
Ping Zhang ◽  
Jing Tan ◽  
Yongzhong Lin
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Disease Model ◽  
Morphological Characteristics ◽  
Microvascular Endothelial Cells ◽  
Alkaline Phosphatase Staining ◽  
Microvascular Endothelial ◽  
Induced Pluripotent

AbstractBackgroundComplications of central nervous system (CNS) in type 2 diabetes mellitus (T2DM) often lead to cognitive impairment and seriously affect the quality of life. However, there is no individualized disease model. Urinary epithelial cells (UECs) can be an ideal source for generating human induced pluripotent stem cells (hiPSCs) and progenitors, as they are easily accessible, non-invasive and universally available. Therefore, we intended to differentiate urine-derived hiPSCs into neuron (N), astrocyte (A) and microvascular endothelial cells (E) from a T2DM patient for future study its pathogenesis and precision medical treatment.Methods and ResultshiPSCs was successfully induced from UECs using integration free Sendai virus technology in a totally noninvasive manner. It had a normal karyotype (46, XY) and were proved to be pluripotent by immunofluorescence staining, alkaline phosphatase staining, karyotyping, teratoma experiments and methylated analysis. N, A and E were successfully induced and displayed typical morphological characteristics.ConclusionsThis study indicates that N, A, E can be generated from urine-derived hiPSCs. Then we intend to create a new disease model in vitro to simulate the cerebral microenvironment of DM which will provide new methods for further investigate the disease-specific mechanisms.

Engineering vascularized flaps using adipose-derived microvascular endothelial cells and mesenchymal stem cells

2017 ◽  
Vol 12 (1) ◽  
pp. e130-e141 ◽  
Author(s):  
Alina Freiman ◽  
Yulia Shandalov ◽  
Dekel Rosenfeld ◽  
Erez Shor ◽  
Dror Ben-David ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial
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