Effects of a saxitoxin-producer strain ofCylindrospermopsis raciborskii (cyanobacteria) on the swimming movements of cladocerans

We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified asStreptomycessp. IMV-70. In the process of fermentation, the strainStreptomycesspp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.

Download Full-text

Molecular analysis of the replication region of the pCIZ2 plasmid from the multiple bacteriocin producer strain Enterococcus faecium L50

Plasmid ◽

10.1016/j.plasmid.2008.07.002 ◽

2008 ◽

Vol 60 (3) ◽

pp. 181-189 ◽

Cited By ~ 3

Author(s):

R. Criado ◽

J. Gutiérrez ◽

A. Budin-Verneuil ◽

P.E. Hernández ◽

A. Hartke ◽

...

Keyword(s):

Molecular Analysis ◽

Enterococcus Faecium ◽

Producer Strain ◽

Bacteriocin Producer ◽

Replication Region

Download Full-text

Efficacy of cell free supernatant from Bacillus subtilis ET-1, an Iturin A producer strain, on biocontrol of green and gray mold

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2017.08.001 ◽

2017 ◽

Vol 134 ◽

pp. 5-10 ◽

Cited By ~ 18

Author(s):

Alfredo Ambrico ◽

Mario Trupo

Keyword(s):

Bacillus Subtilis ◽

Gray Mold ◽

Producer Strain ◽

Iturin A ◽

Cell Free Supernatant

Download Full-text

New Protein Vector ApE1 for Targeted Delivery of Anticancer Drugs

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/469756 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

N. V. Pozdniakova ◽

N. V. Gorokhovets ◽

N. V. Gukasova ◽

A. V. Bereznikova ◽

E. S. Severin

Keyword(s):

Targeted Delivery ◽

Tumor Targeting ◽

Drug Loading ◽

Bacterial Biomass ◽

Receptor Binding Domain ◽

Producer Strain ◽

E Coli ◽

Cytotoxic Compound ◽

Soluble State ◽

New Protein

A new chimeric geneApE1encoding the receptor-binding domain of the humanalpha-fetoprotein fused to a sequence of 22 glutamic acid residues was constructed. A new bacterial producer strainE. coliSHExT7 ApE1 was selected for ApE1 production in a soluble state. A simplified method was developed to purify ApE1 from bacterial biomass. It was shown that the new vector protein selectively interacts with AFP receptors on the tumor cell surface and can be efficiently accumulated in tumor cells. In addition, ApE1 was shown to be stable in storage and during its chemical modification. An increased number of carboxyl groups in the molecule allows the production of cytotoxic compound conjugates with higher drug-loading capacity and enhanced tumor targeting potential.

Download Full-text

Analysis and validation of the pho regulon in the tacrolimus-producer strain Streptomyces tsukubaensis: differences with the model organism Streptomyces coelicolor

Applied Microbiology and Biotechnology ◽

10.1007/s00253-018-9140-0 ◽

2018 ◽

Vol 102 (16) ◽

pp. 7029-7045 ◽

Cited By ~ 3

Author(s):

Miriam Martínez-Castro ◽

Carlos Barreiro ◽

Juan F. Martín

Keyword(s):

Streptomyces Coelicolor ◽

Model Organism ◽

Pho Regulon ◽

Producer Strain ◽

Streptomyces Tsukubaensis

Download Full-text

Identification of an Isolate of Pseudomonas aeroginosa Deposited in PTCC as a PHA Producer Strain: Comparison of Three Different Bacterial Genomic DNA Extraction Methods

Journal of Biological Sciences ◽

10.3923/jbs.2008.826.830 ◽

2008 ◽

Vol 8 (4) ◽

pp. 826-830 ◽

Cited By ~ 1

Author(s):

Hamid Mir Mohammad Sadeghi ◽

Ali Jahanian Najafabadi ◽

Daryoush Abedi ◽

Abbas Jafarian Dehkordi

Keyword(s):

Dna Extraction ◽

Genomic Dna ◽

Extraction Methods ◽

Producer Strain ◽

Genomic Dna Extraction ◽

Strain Comparison ◽

Dna Extraction Methods

Download Full-text

Antitumor Anthraquinones from an Easter Island Sea Anemone: Animal or Bacterial Origin?

Marine Drugs ◽

10.3390/md17030154 ◽

2019 ◽

Vol 17 (3) ◽

pp. 154 ◽

Cited By ~ 3

Author(s):

Ignacio Sottorff ◽

Sven Künzel ◽

Jutta Wiese ◽

Matthias Lipfert ◽

Nils Preußke ◽

...

Keyword(s):

Natural Products ◽

Secondary Metabolites ◽

Antitumor Activity ◽

Genome Analysis ◽

New Genus ◽

Marine Invertebrate ◽

Easter Island ◽

Sea Anemone ◽

Producer Strain ◽

Marine Actinobacteria

The presence of two known anthraquinones, Lupinacidin A and Galvaquinone B, which have antitumor activity, has been identified in the sea anemone (Gyractis sesere) from Easter Island. So far, these anthraquinones have been characterized from terrestrial and marine Actinobacteria only. In order to identify the anthraquinones producer, we isolated Actinobacteria associated with the sea anemone and obtained representatives of seven actinobacterial genera. Studies of cultures of these bacteria by HPLC, NMR, and HRLCMS analyses showed that the producer of Lupinacidin A and Galvaquinone B indeed was one of the isolated Actinobacteria. The producer strain, SN26_14.1, was identified as a representative of the genus Verrucosispora. Genome analysis supported the biosynthetic potential to the production of these compounds by this strain. This study adds Verrucosispora as a new genus to the anthraquinone producers, in addition to well-known species of Streptomyces and Micromonospora. By a cultivation-based approach, the responsibility of symbionts of a marine invertebrate for the production of complex natural products found within the animal’s extracts could be demonstrated. This finding re-opens the debate about the producers of secondary metabolites in sea animals. Finally, it provides valuable information about the chemistry of bacteria harbored in the geographically-isolated and almost unstudied, Easter Island.

Download Full-text