Determination of the acyl moieties of the antibiotic complex A40926 and their relation with the membrane lipids of the producer strain

1992 ◽  
Vol 6 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Luigi F. Zerilli ◽  
Duncan M. F. Edwards ◽  
Angelo Borghi ◽  
Gian G. Gallo ◽  
Enrico Selva ◽  
...  
Keyword(s):  
Membrane Lipids ◽  
Producer Strain

scholarly journals Characterization of the Antibiotic Compound No. 70 Produced byStreptomycessp. IMV-70

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Lyudmila P. Trenozhnikova ◽  
Almagul K. Khasenova ◽  
Assya S. Balgimbaeva ◽  
Galina B. Fedorova ◽  
Genrikh S. Katrukha ◽  
...  
Keyword(s):  
Optimal Growth ◽  
Growth Conditions ◽  
Culture Broth ◽  
Producer Strain ◽  
New Class ◽  
New Antibiotics ◽  
Actinomycete Strain ◽  
Main Component

We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified asStreptomycessp. IMV-70. In the process of fermentation, the strainStreptomycesspp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.

The effect of ozone on leaf cell membranes

1973 ◽  
Vol 51 (6) ◽  
pp. 1213-1219 ◽  
Author(s):  
Elizabeth S. Swanson ◽  
William W. Thomson ◽  
J. Brian Mudd
Keyword(s):  
Fatty Acids ◽  
Cell Membranes ◽  
Unsaturated Fatty Acids ◽  
Membrane Lipids ◽  
Ozone Treatment ◽  
Leaf Cell ◽  
Electron Microscopic ◽  
Unsaturated Lipids ◽  
Significant Difference

The objective of this study was to determine the effects of ozone on membrane lipids and on the electron-density patterns of cell membranes in electron micrographs. Analysis of fatty acids from tobacco leaves fumigated with ozone indicated that there was no significant difference between the ozone-treated and the control plants in the relative amounts of the fatty acids. This suggests that if the primary site of ozone action is unsaturated lipids in membranes then the amounts of affected unsaturated fatty acids are too small to be detected by gas chromatography. In support of this, characteristic electron-microscopic images of membranes are observed in cells of fumigated leaves. However, measurements of the length and width of the chloroplasts and the determination of axial ratios indicated that the ozone treatment resulted in a shrinkage of the chloroplasts. In contrast, mitochondrial changes are apparently explained in terms of ozone-induced swelling.

Total membrane lipid assay (MLA): simple and practical quantification of exosomes based on efficient membrane-specific dyes unaffected by proteins

2018 ◽  
Vol 2 (11) ◽  
pp. 2130-2139 ◽  
Author(s):  
Ning Li ◽  
Zhenlong Huang ◽  
Zhiwei Ye ◽  
Xinfu Zhang ◽  
Lingcheng Chen ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Total Membrane

MLA uses two fluorescent dyes for ratiometric determination of exosomes' membrane lipids.

Determination of glycerolipid composition of rice and maize tissues using solid-phase extraction

2000 ◽  
Vol 28 (6) ◽  
pp. 586-589 ◽  
Author(s):  
I. Rizov ◽  
A. Doulis
Keyword(s):  
Solid Phase Extraction ◽  
Membrane Lipids ◽  
Solid Phase ◽  
Phase Extraction ◽  
Rapid Separation ◽  
Rice Roots ◽  
Maize Leaves ◽  
Glycerolipid Composition

Currently available techniques for the separation and characterization of different glycerolipids are complicated and/or time consuming. By modulating the stationary phase in a solid-phase extraction (SPE) manifold, efficient and rapid separation of plant membrane lipids was achieved. The glycerolipids from rice and maize tissues were separated into seven classes (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylethanolamine, phosphatidylcholine, sulphoquinovosyldiacylglycerol, phosphatidylinositol and phosphatidylglycerol). The pigments present in the rice and maize leaves and rice stems were successfully removed from the total lipid extracts. Pigment-free plant tissue (rice roots) was also analysed. The fatty acid profile of each lipid class isolated by SPE agreed well with those obtained by other separation techniques. The recovery of glycerolipids was at least 87°.

The direct action of hyaluronic acid on human U-937 and HL-60 cells – modification of native and model membranes

Biologia ◽  
2016 ◽  
Vol 71 (11) ◽  
Author(s):  
Anna Barbasz ◽  
Barbara Kreczmer ◽  
Barbara Dyba ◽  
Maria Filek ◽  
Elżbieta Rudolphi-Skórska
Keyword(s):  
Hyaluronic Acid ◽  
Membrane Lipids ◽  
Direct Action ◽  
Model Membranes ◽  
Human Immune System ◽  
Molecular Weights ◽  
Differentiated Cells ◽  
Specific Receptors ◽  
Human Immune

AbstractThe aim of the study was to investigate the effect of application of exogenous hyaluronic acid (HA) of various molecular weights on the cells of human immune system. Two cell lines HL-60 and U-937 with various ability to differentiation were chosen. HL-60 cells were differentiated to macrophages and granulocytes, whereas U-937 only to macrophages. For all investigated cell systems the most cytotoxic effect, indicated as a decrease of cell viability, was found at HA dose equal to 200 mg/L. However, greater effect was observed for differentiated cells and at longer exposure to HA. The possibility of HA interaction with both specific receptors and membrane lipids was tested by determination of biotin-labelled HA binding to cell surface and analysis of physicochemical parameters of model membranes.

scholarly journals Mass spectrometric detection of cross-linked fatty acids formed during radical-induced lesion of lipid membranes

1989 ◽  
Vol 260 (3) ◽  
pp. 873-878 ◽  
Author(s):  
H Frank ◽  
D Thiel ◽  
J MacLeod
Keyword(s):  
Fatty Acids ◽  
Endoplasmic Reticulum ◽  
Lipid Membranes ◽  
Membrane Lipids ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Cell Toxicity ◽  
Significant Process

A mass spectrometric method is described for the quantitative determination of dimers of polyunsaturated fatty acids (PUFA) formed in the hepatic endoplasmic reticulum of rats upon inhalation of tetrachloromethane. The results show that dimers account for a considerable fraction of microsomal PUFA which disappear during CCl4 metabolism. Cross-linking of the membrane lipids of the endoplasmic reticulum seems to be a significant process with respect to cell toxicity.

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

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