Comparing telemetry and fecal dna sampling methods to quantify survival and dispersal of juvenile pygmy rabbits

2015 ◽  
Vol 39 (2) ◽  
pp. 413-421 ◽  
Author(s):  
Stephanie M. Demay ◽  
Janet L. Rachlow ◽  
Lisette P. Waits ◽  
Penny A. Becker
Keyword(s):  
Sampling Methods ◽  
Fecal Dna ◽  
Dna Sampling ◽  
Pygmy Rabbits

Non-Invasive DNA Sampling for Molecular Analysis of Beta-Thalassemia: Amiable Alternative Sampling Methods with Accurate Results for Pediatric Patients

2015 ◽  
Vol 61 (09/2015) ◽  
Author(s):  
Mohd Rahim ◽  
Siew-Leng Kho ◽  
Umah Kuppusamy ◽  
Jin-Ai Tan
Keyword(s):  
Molecular Analysis ◽  
Pediatric Patients ◽  
Sampling Methods ◽  
Beta Thalassemia ◽  
Non Invasive ◽  
Dna Sampling

Comparing morphological and molecular diet analyses and fecal DNA sampling protocols for a terrestrial carnivore

2017 ◽  
Vol 41 (2) ◽  
pp. 362-369 ◽  
Author(s):  
Elyce N. Gosselin ◽  
Robert C. Lonsinger ◽  
Lisette P. Waits
Keyword(s):  
Fecal Dna ◽  
Sampling Protocols ◽  
Dna Sampling ◽  
Diet Analyses ◽  
Terrestrial Carnivore

scholarly journals A Minimally Destructive Protocol for DNA Extraction from Ancient Teeth

2020 ◽  
Author(s):  
Éadaoin Harney ◽  
Olivia Cheronet ◽  
Daniel M. Fernandes ◽  
Kendra Sirak ◽  
Matthew Mah ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Ancient Dna ◽  
Sampling Methods ◽  
Uv Light ◽  
Tooth Surface ◽  
Destructive Sampling ◽  
Morphological Studies ◽  
Tooth Roots ◽  
Dna Sampling ◽  
The Impact

ABSTRACTAncient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive sampling of the skeletal remains from which ancient DNA is obtained. Due to a particularly high concentration of endogenous DNA, the cementum of tooth roots is often targeted for ancient DNA sampling, but standard destructive sampling methods often result in the loss of at least one entire root. Here, we present a minimally destructive method for extracting ancient DNA from dental cementum present on the surface of tooth roots. This method does not require destructive drilling or grinding, and, following extraction, the tooth remains safe to handle and suitable for most morphological studies, as well as other biochemical studies, such as radiocarbon dating. We extracted and sequenced ancient DNA from 30 teeth (and 9 corresponding petrous bones) using this minimally destructive extraction method in addition to a typical tooth sampling method. We find that the minimally destructive method can provide ancient DNA that is of comparable quality to extracts produced from teeth that have undergone destructive sampling processes. Further, we find that a rigorous cleaning of the tooth surface combining diluted bleach and UV light irradiation seems sufficient to minimize external contaminants usually removed through the physical removal of a superficial layer when sampling through regular powdering methods.

Going Beyond 3-D Imaging: Automated 3-D Montaged image Analysis of Cytological Specimens

1996 ◽  
Vol 54 ◽  
pp. 282-283
Author(s):  
Badrinath Roysam ◽  
Hakan Ancin ◽  
Douglas E. Becker ◽  
Robert W. Mackin ◽  
Matthew M. Chestnut ◽  
...  
Keyword(s):  
Image Analysis ◽  
Structural Changes ◽  
Sampling Methods ◽  
Spatial Clustering ◽  
Three Dimensional ◽  
Field Of View ◽  
Cell Counting ◽  
Depth Of Field ◽  
Tissue Cell ◽  
Tissue Organization

This paper summarizes recent advances made by this group in the automated three-dimensional (3-D) image analysis of cytological specimens that are much thicker than the depth of field, and much wider than the field of view of the microscope. The imaging of thick samples is motivated by the need to sample large volumes of tissue rapidly, make more accurate measurements than possible with 2-D sampling, and also to perform analysis in a manner that preserves the relative locations and 3-D structures of the cells. The motivation to study specimens much wider than the field of view arises when measurements and insights at the tissue, rather than the cell level are needed.The term “analysis” indicates a activities ranging from cell counting, neuron tracing, cell morphometry, measurement of tracers, through characterization of large populations of cells with regard to higher-level tissue organization by detecting patterns such as 3-D spatial clustering, the presence of subpopulations, and their relationships to each other. Of even more interest are changes in these parameters as a function of development, and as a reaction to external stimuli. There is a widespread need to measure structural changes in tissue caused by toxins, physiologic states, biochemicals, aging, development, and electrochemical or physical stimuli. These agents could affect the number of cells per unit volume of tissue, cell volume and shape, and cause structural changes in individual cells, inter-connections, or subtle changes in higher-level tissue architecture. It is important to process large intact volumes of tissue to achieve adequate sampling and sensitivity to subtle changes. It is desirable to perform such studies rapidly, with utmost automation, and at minimal cost. Automated 3-D image analysis methods offer unique advantages and opportunities, without making simplifying assumptions of tissue uniformity, unlike random sampling methods such as stereology.12 Although stereological methods are known to be statistically unbiased, they may not be statistically efficient. Another disadvantage of sampling methods is the lack of full visual confirmation - an attractive feature of image analysis based methods.

Pengaruh Tekanan Waktu, Locus Of Control , Tindakan Supervisi, Dan Materialitas Terhadap Penghentian Prematur Atas Prosedur Audit Studi Kasus Pada Kantor Akuntan Publik Daerah Istimewa Yogyakarta Tahun 2015

2017 ◽  
Vol 24 (1) ◽  
pp. 35-53
Author(s):  
Sulastiningsih Sulastiningsih ◽  
Intan Ayu Candra
Keyword(s):  
Locus Of Control ◽  
Premature Termination ◽  
Sampling Methods ◽  
Time Pressure ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Test Reliability ◽  
Friedman Test ◽  
Independent Variables ◽  
Public Accountant

The purpose of this study is to prove: (1) Time pressure, locus of control, the action of supervision and materiality partially affect the premature termination of the audit procedures (2) Time pressure, locus of control, supervision and materiality simultaneously affect the premature termination on the audit procedures. This research was conducted in Public Accountant firm in Yogyakarta region of which total 12 samples of KAP, by distributing 105 questionnaires, and 57 questionnaires were returned (54%). 34 of the returned questionnaires can be processed (34%). The samples in this study were determined by using non-probability sampling, one of purposive sampling methods. Data analysis consisted of: (1) validity test, reliability test and classical assumption. The result showed that the instruments used are quite reliable and valid (2) multiple linear regression analysis. The results are (a) Some of independent variables partially affect premature termination of the audit procedure, while the action of supervision does not influence premature termination of audit procedures (b) All independent variables influence simultaneously to the premature termination of the audit procedures (c) All independent variables showed that as much as 55% it affects on premature termination of the audit procedures, the rest of it are influenced by other variables. (3) Friedman Test. The result shows that there are order of priority of audit procedures being terminated.

72. Evaluation of Different Sampling Methods for Determining Dust Lead Loading on Residential Carpet Surfaces

2000 ◽  
Author(s):  
Z. Bai ◽  
J. Zhang ◽  
G. Rhoads ◽  
P. Lioy ◽  
S. Tsai ◽  
...  
Keyword(s):  
Sampling Methods

431. Improved Generation and Sampling Methods for Tests Using Mycobacterium Aerosols

1999 ◽  
Author(s):  
N. McCullough ◽  
L. Brosseau ◽  
C. Pilon ◽  
D. Vesley
Keyword(s):  
Sampling Methods
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