Desulfovibrio mexicanus sp. nov., a Sulfate-reducing Bacterium Isolated from an Upflow Anaerobic Sludge Blanket (UASB) Reactor Treating Cheese Wastewaters

Anaerobe ◽  
2000 ◽  
Vol 6 (5) ◽  
pp. 305-312 ◽  
Author(s):  
Guadalupe Hernandez-Eugenio ◽  
Marie-Laure Fardeau ◽  
Bharat K.C. Patel ◽  
Hervé Macarie ◽  
Jean-Louis Garcia ◽  
...  
Keyword(s):  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Anaerobic Sludge Blanket

scholarly journals Microbial Diversity Dynamics in a Methanogenic-Sulfidogenic UASB Reactor

2021 ◽  
Vol 18 (3) ◽  
pp. 1305
Author(s):  
E. Fernández-Palacios ◽  
Xudong Zhou ◽  
Mabel Mora ◽  
David Gabriel
Keyword(s):  
Paper Industry ◽  
Chemical Species ◽  
Methanogenic Archaea ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Sulfate Reducing ◽  
Long Run

In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42−L−1 and a constant C/S ratio of 1.5 ± 0.3 g Cg−1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB.

scholarly journals Microbial Removal of Pb(II) Using an Upflow Anaerobic Sludge Blanket (UASB) Reactor

Catalysts ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 512
Author(s):  
Jeremiah Chimhundi ◽  
Carla Hörstmann ◽  
Evans M. N. Chirwa ◽  
Hendrik G. Brink
Keyword(s):  
Treatment Process ◽  
Removal Rate ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Reduction Potentials ◽  
Oxidation Reduction ◽  
Anaerobic Sludge Blanket ◽  
Microbial Removal ◽  
Maximum Removal

The main objective of this study was to achieve the continuous biorecovery and bioreduction of Pb(II) using an industrially obtained consortia as a biocatalyst. An upflow anaerobic sludge blanket reactor was used in the treatment process. The bioremediation technique that was applied made use of a yeast extract as the microbial substrate and Pb(NO3)2 as the source of Pb(II). The UASB reactor exhibited removal efficiencies of between 90 and 100% for the inlet Pb concentrations from 80 to 2000 ppm and a maximum removal rate of 1948.4 mg/(L·d) was measured. XRD and XPS analyses of the precipitate revealed the presence of Pb0, PbO, PbS and PbSO4. Supporting experimental work carried out included growth measurements, pH, oxidation–reduction potentials and nitrate levels.

KINETIC MODELS FOR PREDICTION OF COD EFFLUENT FROM UPFLOW ANAEROBIC SLUDGE BLANKET (UASB) REACTOR FOR CANNERY SEAFOOD WASTEWATER TREATMENT

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Sunwanee Jijai ◽  
Chairat Siripatana ◽  
Sompong O-Thong ◽  
Norli Ismail
Keyword(s):  
Palm Oil ◽  
Kinetic Models ◽  
Second Order ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Substrate Removal ◽  
Reactor Kinetic ◽  
Palm Oil Mill ◽  
Anaerobic Sludge Blanket

The three identical lab-scale upflow anaerobic sludge blanket (UASB) reactors were operated continuously for treating cannery seafood wastewater at seven hydraulic retention times (HRTs) of 5, 4, 3, 2, 1, 0.5 and 0.25 days. The different of granule sizes from three sources: a cassava factory (CS), a seafood factory (SS), and a palm oil mill (PS), average sizes in the range 1.5-1.7, 0.7-1.0 and 0.1-0.2 mm respectively were used as inocula for anaerobic digestion. The UASB-R1 used only granules from seafood factory (R1-SS), the UASB-R2 used mixed granules from seafood with cassava factory (R2-SS+CS) and the UASB-R3 used mixed granules from seafood factory with palm oil mill (R3-SS+PS). In this study selected mathematical models including Monod, Contois, Grau second-order and modified Stover-Kicannon kinetic models were applied to determine the substrate removal kinetics of UASB reactor. Kinetic parameters were determined through linear regression using experimental data obtained from the steady-state experiment and subsequently used to predict effluent COD. The results showed that Grau second-order and modified Stover-Kicannon kinetic models were more suitable than that of others for predicting the effluent COD, with high the correlation coefficient (R2). In addition, the UASB-R2 from mixed granules with cassava factory (SS+CS) gave the best performance and highest coefficient value.

Treatment of raw sewage in a temperate climate using a UASB reactor and the hanging sponge cubes process

1997 ◽  
Vol 36 (6-7) ◽  
pp. 433-440 ◽  
Author(s):  
Lalit K. Agrawal ◽  
Yasuhiro Ohashi ◽  
Etsuo Mochida ◽  
Hiroyuki Okui ◽  
Yasuko Ueki ◽  
...  
Keyword(s):  
Ammonia Oxidation ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Temperate Climate ◽  
Anaerobic Sludge ◽  
Treatment Efficiency ◽  
Oxidation Rates ◽  
Air Intake ◽  
Anaerobic Sludge Blanket ◽  
Digested Sewage Sludge

The treatability of raw sewage in a temperate climate (wintertime around 10–20°C) using an upflow anaerobic sludge blanket (UASB) reactor and the hanging sponge cubes process was evaluated. After being seeded with digested sewage sludge, a 47.1 L UASB reactor was continuously operated for more than 2 years by feeding raw sewage, which had average COD around 300 mg/L (41% soluble). During summertime at an HRT of 7 h, effluent COD approximately 70 mg/L total, 50 mg/L soluble and BOD5 20 mg/L total, 12 mg/L soluble was obtained. During wintertime also, treatment efficiency and process stability was good. With the hanging sponge cubes process using the effluent of the UASB reactor treating raw sewage, the following results were obtained. The ammonia oxidation rates of 1.9 and 3.5 g NH4-N·m−2·d−1 in a downflow hanging sponge cubes biofilter, under natural air intake only were obtained during wintertime and summertime, respectively. With post-denitrification and an external carbon source, 84% in average N (NO3+NO2) was removed with an HRT of less than 1 hour and in the temperature range of 13 to 30°C using an upflow submerged hanging sponge bed bioreactor, under anaerobic conditions. The overall system using a UASB reactor and the hanging sponge cubes process could be quite an attractive treatment alternative.

scholarly journals Organic Matter Removal in a Simultaneous Nitrification-Denitrification Process Using Fixed-Film System.

2021 ◽  
Author(s):  
Perla Gonzalez ◽  
Ana Aguilar Ruiz ◽  
Andrea Reynosa Varela ◽  
Ulises Durán Hinojosa ◽  
Marco Garzón Zuñiga ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nitrogen Compound ◽  
Upflow Anaerobic Sludge Blanket ◽  
Swine Wastewater ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Organic Matter Removal ◽  
Fixed Film ◽  
Suspended Biomass ◽  
Anaerobic Sludge Blanket

Abstract This study focused on evaluating different support media for COD and nitrogen compound removal from an Upflow Anaerobic Sludge Blanket (UASB) reactor fed with swine wastewater. Maximum specific nitrification (MSNA) and denitrification (MSDA) activity tests were performed in two fixed-film systems with (1) polyurethane foam (R1) and (2) polyethylene rings (R2). The results showed that the R2 system performed more efficiently than R1, reaching organic matter removal of 77 ± 8% and nitrogen of 98 ± 4%, attributed to higher specific denitrifying activity recorded (5.3 ± 0.34 g NO3--N/g VTS ∙h). In this sense, MSDA tests indicated that the suspended biomass was responsible for at least 70% of nitrogen removal in the form of ammonium compared with 20% attributed to biomass in the form of biofilm. On the other hand, 40 ± 5% of initial nitrogen could not be quantified in the system effluents, but 10 ± 1% was attributed to loss by volatilization. According to the analyses, the previous information infers the development of simultaneous nitrification-denitrification (SND) routes. Respect to the analyses of microbial diversity and abundance in the biofilm of R2 rings, the presence of the genus Pseudomonas dominated the prokaryotic community of the system in 54.4%.

Acid Endurance and Sorption of Zn2+ of Anaerobic Granular Sludge Acclimated by Flue Gas Pretreatment Wastewater

2013 ◽  
Vol 634-638 ◽  
pp. 182-186
Author(s):  
Juan Wang ◽  
Qin Zhong
Keyword(s):  
Flue Gas ◽  
Ph Value ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anaerobic Granular Sludge ◽  
Methanogenic Activity ◽  
Activity Inhibition ◽  
Anaerobic Sludge Blanket

With the aim to use anaerobic granular sludge, the methanogenic activity inhibition and recovery of anaerobic granular sludge from an industrial anaerobic reactor (s1) were investigated by measuring the methane volume at low pH. A lab-scale upflow anaerobic sludge blanket (UASB) reactor was inoculated with s1.s1 was used to remove Zn2+ in wastewater. The results show that activity of s1 is similar when the pH value is 6.5 to 7.0. The methane volume is obviously decreased when the pH value is 6.0. The activity is completely inhibited when the pH value is 4.5. The activity is fully recovered when the pH is above 6.5 and hardly recovers when the pH fell to 4.5. The main Zn2+ removal mechanism is chemical adsorption.

Impact of microaeration bioreactor on dissolved sulfide and methane removal from real UASB effluent for sewage treatment

2020 ◽  
Vol 81 (9) ◽  
pp. 1951-1960 ◽  
Author(s):  
C. S. Cabral ◽  
A. L. Sanson ◽  
R. J. C. F. Afonso ◽  
C. A. L. Chernicharo ◽  
J. C. Araújo
Keyword(s):  
Sewage Treatment ◽  
Oxygen Demand ◽  
Post Treatment ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Sulfide Removal ◽  
Dissolved Sulfide ◽  
Removal Efficiencies ◽  
Anaerobic Sludge Blanket

Abstract Two bioreactors were investigated as an alternative for the post-treatment of effluent from an upflow anaerobic sludge blanket (UASB) reactor treating domestic sewage, aiming at dissolved sulfide and methane removal. The bioreactors (R-control and R-air) were operated at different hydraulic retention times (HRT; 6 and 3 h) with or without aeration. Large sulfide and methane removal efficiencies were achieved by the microaerated reactor at HRT of 6 h. At this HRT, sulfide removal efficiencies were equal to 61% and 79%, and methane removal efficiencies were 31% and 55% for R-control and R-air, respectively. At an HRT of 3 h, sulfide removal efficiencies were 22% (R-control) and 33% (R-air) and methane removal did not occur. The complete oxidation of sulfide, with sulfate formation, prevailed in both phases and bioreactors. However, elemental sulfur formation was more predominant at an HRT of 6 h than at an HRT of 3 h. Taken together, the results show that post-treatment improved the anaerobic effluent quality in terms of chemical oxygen demand and solids removal. However, ammoniacal nitrogen was not removed due to either the low concentration of air provided or the absence of microorganisms involved in the nitrogen cycle.

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