scholarly journals Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.

2002 ◽  
Vol 52 (5) ◽  
pp. 1461-1468 ◽  
Author(s):  
Guadalupe Hernández-Eugenio ◽  
Marie-Laure Fardeau ◽  
Jean-Luc Cayol ◽  
Bharat K C Patel ◽  
Pierre Thomas ◽  
...  
Catalysts ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 512
Author(s):  
Jeremiah Chimhundi ◽  
Carla Hörstmann ◽  
Evans M. N. Chirwa ◽  
Hendrik G. Brink

The main objective of this study was to achieve the continuous biorecovery and bioreduction of Pb(II) using an industrially obtained consortia as a biocatalyst. An upflow anaerobic sludge blanket reactor was used in the treatment process. The bioremediation technique that was applied made use of a yeast extract as the microbial substrate and Pb(NO3)2 as the source of Pb(II). The UASB reactor exhibited removal efficiencies of between 90 and 100% for the inlet Pb concentrations from 80 to 2000 ppm and a maximum removal rate of 1948.4 mg/(L·d) was measured. XRD and XPS analyses of the precipitate revealed the presence of Pb0, PbO, PbS and PbSO4. Supporting experimental work carried out included growth measurements, pH, oxidation–reduction potentials and nitrate levels.


2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Sunwanee Jijai ◽  
Chairat Siripatana ◽  
Sompong O-Thong ◽  
Norli Ismail

The three identical lab-scale upflow anaerobic sludge blanket (UASB) reactors were operated continuously for treating cannery seafood wastewater at seven hydraulic retention times (HRTs) of 5, 4, 3, 2, 1, 0.5 and 0.25 days. The different of granule sizes from three sources: a cassava factory (CS), a seafood factory (SS), and a palm oil mill (PS), average sizes in the range 1.5-1.7, 0.7-1.0 and 0.1-0.2 mm respectively were used as inocula for anaerobic digestion. The UASB-R1 used only granules from seafood factory (R1-SS), the UASB-R2 used mixed granules from seafood with cassava factory (R2-SS+CS) and the UASB-R3 used mixed granules from seafood factory with palm oil mill (R3-SS+PS). In this study selected mathematical models including Monod, Contois, Grau second-order and modified Stover-Kicannon kinetic models were applied to determine the substrate removal kinetics of UASB reactor. Kinetic parameters were determined through linear regression using experimental data obtained from the steady-state experiment and subsequently used to predict effluent COD. The results showed that Grau second-order and modified Stover-Kicannon kinetic models were more suitable than that of others for predicting the effluent COD, with high the correlation coefficient (R2). In addition, the UASB-R2 from mixed granules with cassava factory (SS+CS) gave the best performance and highest coefficient value.


1997 ◽  
Vol 36 (6-7) ◽  
pp. 433-440 ◽  
Author(s):  
Lalit K. Agrawal ◽  
Yasuhiro Ohashi ◽  
Etsuo Mochida ◽  
Hiroyuki Okui ◽  
Yasuko Ueki ◽  
...  

The treatability of raw sewage in a temperate climate (wintertime around 10–20°C) using an upflow anaerobic sludge blanket (UASB) reactor and the hanging sponge cubes process was evaluated. After being seeded with digested sewage sludge, a 47.1 L UASB reactor was continuously operated for more than 2 years by feeding raw sewage, which had average COD around 300 mg/L (41% soluble). During summertime at an HRT of 7 h, effluent COD approximately 70 mg/L total, 50 mg/L soluble and BOD5 20 mg/L total, 12 mg/L soluble was obtained. During wintertime also, treatment efficiency and process stability was good. With the hanging sponge cubes process using the effluent of the UASB reactor treating raw sewage, the following results were obtained. The ammonia oxidation rates of 1.9 and 3.5 g NH4-N·m−2·d−1 in a downflow hanging sponge cubes biofilter, under natural air intake only were obtained during wintertime and summertime, respectively. With post-denitrification and an external carbon source, 84% in average N (NO3+NO2) was removed with an HRT of less than 1 hour and in the temperature range of 13 to 30°C using an upflow submerged hanging sponge bed bioreactor, under anaerobic conditions. The overall system using a UASB reactor and the hanging sponge cubes process could be quite an attractive treatment alternative.


2021 ◽  
Author(s):  
Perla Gonzalez ◽  
Ana Aguilar Ruiz ◽  
Andrea Reynosa Varela ◽  
Ulises Durán Hinojosa ◽  
Marco Garzón Zuñiga ◽  
...  

Abstract This study focused on evaluating different support media for COD and nitrogen compound removal from an Upflow Anaerobic Sludge Blanket (UASB) reactor fed with swine wastewater. Maximum specific nitrification (MSNA) and denitrification (MSDA) activity tests were performed in two fixed-film systems with (1) polyurethane foam (R1) and (2) polyethylene rings (R2). The results showed that the R2 system performed more efficiently than R1, reaching organic matter removal of 77 ± 8% and nitrogen of 98 ± 4%, attributed to higher specific denitrifying activity recorded (5.3 ± 0.34 g NO3--N/g VTS ∙h). In this sense, MSDA tests indicated that the suspended biomass was responsible for at least 70% of nitrogen removal in the form of ammonium compared with 20% attributed to biomass in the form of biofilm. On the other hand, 40 ± 5% of initial nitrogen could not be quantified in the system effluents, but 10 ± 1% was attributed to loss by volatilization. According to the analyses, the previous information infers the development of simultaneous nitrification-denitrification (SND) routes. Respect to the analyses of microbial diversity and abundance in the biofilm of R2 rings, the presence of the genus Pseudomonas dominated the prokaryotic community of the system in 54.4%.


2013 ◽  
Vol 634-638 ◽  
pp. 182-186
Author(s):  
Juan Wang ◽  
Qin Zhong

With the aim to use anaerobic granular sludge, the methanogenic activity inhibition and recovery of anaerobic granular sludge from an industrial anaerobic reactor (s1) were investigated by measuring the methane volume at low pH. A lab-scale upflow anaerobic sludge blanket (UASB) reactor was inoculated with s1.s1 was used to remove Zn2+ in wastewater. The results show that activity of s1 is similar when the pH value is 6.5 to 7.0. The methane volume is obviously decreased when the pH value is 6.0. The activity is completely inhibited when the pH value is 4.5. The activity is fully recovered when the pH is above 6.5 and hardly recovers when the pH fell to 4.5. The main Zn2+ removal mechanism is chemical adsorption.


2020 ◽  
Vol 81 (9) ◽  
pp. 1951-1960 ◽  
Author(s):  
C. S. Cabral ◽  
A. L. Sanson ◽  
R. J. C. F. Afonso ◽  
C. A. L. Chernicharo ◽  
J. C. Araújo

Abstract Two bioreactors were investigated as an alternative for the post-treatment of effluent from an upflow anaerobic sludge blanket (UASB) reactor treating domestic sewage, aiming at dissolved sulfide and methane removal. The bioreactors (R-control and R-air) were operated at different hydraulic retention times (HRT; 6 and 3 h) with or without aeration. Large sulfide and methane removal efficiencies were achieved by the microaerated reactor at HRT of 6 h. At this HRT, sulfide removal efficiencies were equal to 61% and 79%, and methane removal efficiencies were 31% and 55% for R-control and R-air, respectively. At an HRT of 3 h, sulfide removal efficiencies were 22% (R-control) and 33% (R-air) and methane removal did not occur. The complete oxidation of sulfide, with sulfate formation, prevailed in both phases and bioreactors. However, elemental sulfur formation was more predominant at an HRT of 6 h than at an HRT of 3 h. Taken together, the results show that post-treatment improved the anaerobic effluent quality in terms of chemical oxygen demand and solids removal. However, ammoniacal nitrogen was not removed due to either the low concentration of air provided or the absence of microorganisms involved in the nitrogen cycle.


2012 ◽  
Vol 65 (10) ◽  
pp. 1887-1894 ◽  
Author(s):  
V. Singh ◽  
A. K. Mittal

This study reports applicability of upflow anaerobic sludge blanket (UASB) process to treat the leachate from a municipal landfill located in Delhi. A laboratory scale reactor was operated at an organic loading rate of 3.00 kg chemical oxygen demand (COD)/m3 d corresponding to a hydraulic retention time (HRT) of 12 h for over 8 months. The effect of toxicity of leachate, and feed composition on the treatability of leachate was evaluated. Average COD of the leachate, during the study period varied between 8,880 and 66,420 mg/l. Toxicity of the leachate used during a period of 8 months varied from LC50 1.22 to 12.35 for 96 h. The removal efficiency of soluble COD ranged between 91 and 67% for fresh leachate and decreased drastically from 90 to 35% for old leachate having high toxicity. The efficiency varied from 81 to 65%. The reactor performed more efficiently for the treatment of fresh leachate (less toxic, LC50 11.64, 12.35, and 12.15 for 96 h) as compared with old leachate (more toxic, LC50 1.22 for 96 h). Toxicity of the leachate affected its treatment potential by the UASB.


1986 ◽  
Vol 18 (12) ◽  
pp. 99-108 ◽  
Author(s):  
Gatze Lettinga ◽  
Look Hulshoff Pol

Of the high rate anaerobic wastewater treatment systems the UASB (Upflow Anaerobic Sludge Blanket) reactor has found the widest application. Therefore the attention with respect to design, operation and economy will be focussed on this reactor type. In designing a UASB reactor specific attention is needed for the GSS (Gas-Solids Separator) device and the feed inlet system. For soluble wastewater generally no phase separation is required. Only for wastewaters high in suspended solids pre-acidification in a separate acidification reactor can be beneficial. Increasing attention is given to the development of modified UASB systems, such as a combination of a sludge bed reactor and an anaerobic filter. Other possible modified UASB systems may be found in a FS (Floating Settling) UASB reactor, the EGSB (Expanded Granular Sludge Bed) reactor and the UASB IC (Internal Circulation) reactor. As many factors are involved in the costs of a UASB reactor, only some rough data on reactor costs are presented.


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