Impact of Change in Fermentation Process pH and Dissolved Carbon Dioxide Concentration on Secreted Antibody Structure and Cell Physiology of a GS-NS0 Cell Line Expressing a Human Antibody

2005 ◽  
pp. 637-640
Author(s):  
Alison Ridley ◽  
Jonathan Dempsey ◽  
Chris Gee ◽  
Richard Turner ◽  
Matthew Osborne ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Cell Line ◽  
Fermentation Process ◽  
Carbon Dioxide Concentration ◽  
Cell Physiology ◽  
Human Antibody ◽  
Dissolved Carbon ◽  
Dissolved Carbon Dioxide ◽  
Antibody Structure

scholarly journals Development of a Lipase Fermentation Process That Uses a Recombinant Pseudomonas alcaligenes Strain

1998 ◽  
Vol 64 (7) ◽  
pp. 2644-2651 ◽  
Author(s):  
Gijs Gerritse ◽  
Ronald W. J. Hommes ◽  
Wim J. Quax
Keyword(s):  
Carbon Dioxide ◽  
Copy Number ◽  
Fermentation Process ◽  
Scale Up ◽  
Carbon Dioxide Concentration ◽  
Gene Copy ◽  
Alkaline Lipase ◽  
Dissolved Carbon ◽  
Dissolved Carbon Dioxide ◽  
Pseudomonas Alcaligenes

ABSTRACT Pseudomonas alcaligenes M-1 secretes an alkaline lipase, which has excellent characteristics for the removal of fatty stains under modern washing conditions. A fed-batch fermentation process based on the secretion of the alkaline lipase from P. alcaligenes was developed. Due to the inability of P. alcaligenes to grow on glucose, citric acid and soybean oil were applied as substrates in the batch phase and feed phase, respectively. The gene encoding the high-alkaline lipase from P. alcaligenes was isolated and characterized. Amplification of lipase gene copies in P. alcaligenes with the aid of low- and high-copy-number plasmids resulted in an increase of lipase expression that was apparently colinear with the gene copy number. It was found that overexpression of the lipase helper gene,lipB, produced a stimulating effect in strains with high copy numbers (>20) of the lipase structural gene, lipA. In strains with lipA on a low-copy-number vector, thelipB gene did not show any effect, suggesting that LipB is required in a low ratio to LipA only. During scaling up of the fermentation process to 100 m3, severe losses in lipase productivity were observed. Simulations have identified an increased level of dissolved carbon dioxide as the most probable cause for the scale-up losses. A large-scale fermentation protocol with a reduced dissolved carbon dioxide concentration resulted in a substantial elimination of the scale-up loss.

Impact of dissolved carbon dioxide concentration on the process parameters during its conversion to acetate through microbial electrosynthesis

2018 ◽  
Vol 3 (3) ◽  
pp. 371-378 ◽  
Author(s):  
Gunda Mohanakrishna ◽  
Karolien Vanbroekhoven ◽  
Deepak Pant
Keyword(s):  
Carbon Dioxide ◽  
Greenhouse Gases ◽  
Process Parameters ◽  
Carbon Fixation ◽  
Carbon Dioxide Concentration ◽  
Value Added ◽  
Microbial Electrosynthesis ◽  
Dissolved Carbon ◽  
Dissolved Carbon Dioxide

The reduction of carbon dioxide (CO2) released from industry can help to reduce the emissions of greenhouse gases (GHGs) to the atmosphere while at the same time producing value-added chemicals and contributing to carbon fixation.

Impact of Dursban and Abate on Microbial Numbers and Some Chemical Properties of Standing Ponds

1975 ◽  
Vol 10 (1) ◽  
pp. 33-41 ◽  
Author(s):  
J. Butcher ◽  
M. Boyer ◽  
CD. Fowle
Keyword(s):  
Carbon Dioxide ◽  
Active Ingredient ◽  
Algal Blooms ◽  
Chemical Properties ◽  
Total Carbon ◽  
Dissolved Carbon ◽  
Photosynthetic Productivity ◽  
Dissolved Carbon Dioxide ◽  
Microbial Numbers

Abstract Eleven small ponds, lined with polyethylene, were used to assess the consequences of applications of *DursbanR at 0.004, 0.030, 0.100 and 1.000 ppm and AbateR at 0.025 and 0.100 ppm active ingredient. The treated ponds showed a more pronounced long-term increase in pH and dissolved oxygen and decreasing total and dissolved carbon dioxide in comparison with untreated ponds. Algal blooms were of longer duration in treated ponds than in controls. Total photosynthetic productivity was higher in treated ponds but bacterial numbers did not change significantly. Photosynthetic productivity was estimated by following the changes in total carbon dioxide.

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