Tissue Reconstruction in vivo

Composite biomaterials, like calciumphosphate/bioresorbable polymer, offer excellent potential for reconstruction and reparation of bone tissue defects induced by different sources. In this paper synthesis of calciumphosphate/poly-DL-lactide-co-glycolide (BCP/DLPLG) composite biomaterial formed as filler and blocks was studied. BCP/DLPLG composite biomaterial was produced in the form of spherical granules of BCP covered by a DLPLG layer, average diameter of 150-250 µm. By cold and hot pressing of granules at up to 10000 kg/cm2, blocks with fine distribution of phases and porosity up to 3% were obtained. Characterization was performed by wide-angle X-ray structural analysis (WAXS), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), infrared spectroscopy (IR), and mechanical properties by defining the compressive strength. In vitro citotoxicity research was carried out on cellular cultures of fibroblasts of human (MRC5) and mouse (L929). In vivo research was performed in two steps. Reparatory ability of BCP/DLPLG in mice was examined in the first step, and then bone tissue reconstruction possibilities on 10 patients in the next step. In vitro tests showed very good fibroblast adhesion and non-citotoxicity of the composite. A material is considered non-cytotoxic if the cell survival is above 50 %, and in our case it was 90%. In vivo research on mice indicated high level of reparatory ability of this composite with formation of new bone and vascular tissue six weeks after reparation. Application of this composite for healing infrabone defects of patients showed a high level of osseous regeneration.

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Surface modification of small intestine submucosa in tissue engineering

Regenerative Biomaterials ◽

10.1093/rb/rbaa014 ◽

2020 ◽

Vol 7 (4) ◽

pp. 339-348 ◽

Cited By ~ 1

Author(s):

Pan Zhao ◽

Xiang Li ◽

Qin Fang ◽

Fanglin Wang ◽

Qiang Ao ◽

...

Keyword(s):

Tissue Engineering ◽

Surface Modification ◽

Small Intestine ◽

Cell Adhesion ◽

Great Influence ◽

Small Intestine Submucosa ◽

Proliferation And Differentiation ◽

Tissue Reconstruction

Abstract With the development of tissue engineering, the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo. Especially, surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials. The small intestine submucosa (SIS) is an extracellular matrix isolated from the submucosal layer of porcine jejunum, which has good tissue mechanical properties and regenerative activity, and is suitable for cell adhesion, proliferation and differentiation. In recent years, SIS is widely used in different areas of tissue reconstruction, such as blood vessels, bone, cartilage, bladder and ureter, etc. This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds, including functional group bonding, protein adsorption, mineral coating, topography and formatting modification and drug combination. In addition, the reasonable combination of these methods also offers great improvement on SIS surface modification. This article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

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Self-swelling tissue expander for soft tissue reconstruction in the craniofacial region: An in vitro and in vivo evaluation

Bio-Medical Materials and Engineering ◽

10.3233/bme-211224 ◽

2021 ◽

pp. 1-14

Author(s):

Yili Chang ◽

Fubao Zhang ◽

Feng Liu ◽

Lianshui Shi ◽

Lin Zhang ◽

...

Keyword(s):

Soft Tissue ◽

Tissue Expander ◽

Physical Characteristics ◽

Radiographic Evaluation ◽

Control Group ◽

Soft Tissue Reconstruction ◽

Tissue Reconstruction ◽

Ft Ir

BACKGROUND: Craniofacial soft-tissue defects mostly have an impact on the treatment of various oral diseases. Tissue expander is an important technique for tissue reconstruction, especially for soft tissues in reconstructive surgery. OBJECTIVE: This research aimed to develop a new self-swelling tissue expander, namely hydrogel, for soft tissue reconstruction in craniofacial region. METHODS: In vitro, the chemical and physical characteristics of hydrogel were evaluated by SEM, swelling rate, mechanical testing, EDS, and FT-IR. In vivo, the craniofacial implant model of SD rats were divided into group A as control, group B with hydrogels for 1 week expansion, group C for 2 weeks and group D for 4 weeks (n = 5), and the effects were analyzed by HE staining, histological and radiographic evaluation. RESULTS: The in vitro results suggested that dry hydrogel possessed a uniform surface with micropores, the surface of post-swelling hydrogel formed three-dimensional meshwork. Within 24 hours, hydrogels expanded markedly, then slowed down. The mechanical property of hydrogels with longer expansion was better, whose main elements were carbon and oxygen. FT-IR also verified its molecular structure. In vivo, the wounds of rats recovered well, hydrogels could be removed as one whole piece with original shape and examined by radiographic evaluation, besides, the expanded skin and developed fibrous capsule formed surrounding hydrogels. CONCLUSION: The new expander was designed successfully with good chemical and physical characteristics, and could be applied in an animal model to help tissue reconstruction.

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VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells

Scientific Reports ◽

10.1038/s41598-021-97729-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Kazuko Abe ◽

Shigeyuki Kon ◽

Hiroki Kameyama ◽

JiDong Zhang ◽

Ken-ichirou Morohashi ◽

...

Keyword(s):

Cell Adhesion ◽

Leydig Cells ◽

Interstitial Tissue ◽

Testicular Cells ◽

Aggregate Culture ◽

Tissue Reconstruction ◽

Blocking Antibodies ◽

Cell To Cell Adhesion

AbstractRoles of interstitial tissue in morphogenesis of testicular structures remain less well understood. To analyze the roles of CD34+ cells in the reconstruction of interstitial tissue containing Leydig cells (LCs), and testicular structures, we used 3D-reaggregate culture of dissociated testicular cells from prepubertal mouse. After a week of culture, adult Leydig cells (ALCs) were preferentially incorporated within CD34+ cell-aggregates, but fetal LCs (FLCs) were not. Immunofluorescence studies showed that integrins α4, α9 and β1, and VCAM1, one of the ligands for integrins α4β1 and α9β1, are expressed mainly in CD34+ cells and ALCs, but not in FLCs. Addition of function-blocking antibodies against each integrin and VCAM1 to the culture disturbed the reconstruction of testicular structures. Antibodies against α4 and β1 integrins and VCAM1 robustly inhibited cell-to-cell adhesion between testicular cells and between CD34+ cells. Cell-adhesion assays indicated that CD34+ cells adhere to VCAM1 through the interaction with α4β1 integrin. Live cell imaging showed that CD34+ cells adhered around ALC-aggregates. CD34+ cells on the dish moved toward the aggregates, extending filopodia, and entered into them, which was disturbed by VCAM1 antibody. These results indicate that VCAM1-α4β1 integrin interaction plays pivotal roles in formation of testicular interstitial tissues in vitro and also in vivo.

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Silk based bioinks for soft tissue reconstruction using 3-dimensional (3D) printing with in vitro and in vivo assessments

Biomaterials ◽

10.1016/j.biomaterials.2016.11.046 ◽

2017 ◽

Vol 117 ◽

pp. 105-115 ◽

Cited By ~ 93

Author(s):

María J. Rodriguez ◽

Joseph Brown ◽

Jodie Giordano ◽

Samuel J. Lin ◽

Fiorenzo G. Omenetto ◽

...

Keyword(s):

Soft Tissue ◽

3D Printing ◽

Soft Tissue Reconstruction ◽

3 Dimensional ◽

Tissue Reconstruction

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In vivo Quantification of the Effects of Radiation and Presence of Hair Follicle Pores on the Proliferation of Fibroblasts in an Acellular Human Dermis in a Dorsal Skinfold Chamber: Relevance for Tissue Reconstruction following Neoadjuvant Therapy

PLoS ONE ◽

10.1371/journal.pone.0125689 ◽

2015 ◽

Vol 10 (5) ◽

pp. e0125689 ◽

Cited By ~ 2

Author(s):

Mario Vitacolonna ◽

Djeda Belharazem ◽

Patrick Maier ◽

Peter Hohenberger ◽

Eric Dominic Roessner

Keyword(s):

Hair Follicle ◽

Neoadjuvant Therapy ◽

Dorsal Skinfold Chamber ◽

Human Dermis ◽

Tissue Reconstruction ◽

Effects Of Radiation

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Guided Tissue Regeneration in Heart Valve Replacement: From Preclinical Research to First-in-Human Trials

BioMed Research International ◽

10.1155/2015/432901 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 19

Author(s):

L. Iop ◽

G. Gerosa

Keyword(s):

Heart Valve ◽

Somatic Growth ◽

The Elderly ◽

Preclinical Research ◽

Therapeutic Concept ◽

Clinical Investigations ◽

Tissue Engineering Approach ◽

Tissue Reconstruction ◽

Valve Diseases

Heart valve tissue-guided regeneration aims to offer a functional and viable alternative to current prosthetic replacements. Not requiring previous cell seeding and conditioning in bioreactors, such exceptional tissue engineering approach is a very fascinating translational regenerative strategy. Afterin vivoimplantation, decellularized heart valve scaffolds drive their same repopulation by recipient’s cells for a prospective autologous-like tissue reconstruction, remodeling, and adaptation to the somatic growth of the patient. With such a viability, tissue-guided regenerated conduits can be delivered as off-the-shelf biodevices and possess all the potentialities for a long-lasting resolution of the dramatic inconvenience of heart valve diseases, both in children and in the elderly. A review on preclinical and clinical investigations of this therapeutic concept is provided with evaluation of the issues still to be well deliberated for an effective and safe in-human application.

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Mesoporous calcium silicate nanoparticles for superficial dental tissue reconstruction, in vitro and in vivo

RSC Advances ◽

10.1039/d1ra02114a ◽

2021 ◽

Vol 11 (40) ◽

pp. 24681-24693

Author(s):

Yixue Gao ◽

Pin Huang ◽

Ruiying Chen ◽

Man Wang ◽

Yining Wang ◽

...

Keyword(s):

Drug Delivery ◽

Calcium Silicate ◽

Antibacterial Properties ◽

Dental Tissue ◽

Dentinal Tubules ◽

Tissue Reconstruction

MCSNs could be used as a promising biomaterial for occluding the dentinal tubules in vitro and in vivo. Also, the outstanding drug delivery and antibacterial properties enable it to carry antibiotics easily for inhibiting deeper pulp infection.

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