Microtubules and Viral Movement

2008 ◽  
pp. 141-173 ◽  
Author(s):  
Manfred Heinlein
Keyword(s):  
Viral Movement

Viral Movement Proteins Induce Tubule Formation in Plant and Insect Cells

2006 ◽  
pp. 160-175 ◽  
Author(s):  
Jan W. M. van Lent ◽  
Corinne Schmitt-Keichinger
Keyword(s):  
Insect Cells ◽  
Tubule Formation ◽  
Movement Proteins ◽  
Viral Movement

scholarly journals Pseudomonas syringae Effector avrB Confers Soybean Cultivar-Specific Avirulence on Soybean mosaic virus Adapted for Transgene Expression but Effector avrPto Does Not

2006 ◽  
Vol 19 (3) ◽  
pp. 304-312 ◽  
Author(s):  
Li Wang ◽  
Alan Eggenberger ◽  
John Hill ◽  
Adam J. Bogdanove
Keyword(s):  
Mosaic Virus ◽  
Pseudomonas Syringae ◽  
Transgene Expression ◽  
Fluorescent Protein ◽  
Soybean Mosaic Virus ◽  
Hypersensitive Reaction ◽  
Green Fluorescent Protein Gene ◽  
Avirulence Genes ◽  
Pathogenesis Related Protein ◽  
Viral Movement

Soybean mosaic virus (SMV) was adapted for transgene expression in soybean and used to examine the function of avirulence genes avrB and avrPto of Pseudomonas syringae pvs. glycinea and tomato, respectively. A cloning site was introduced between the P1 and HC-Pro genes in 35S-driven infectious cDNAs of strains SMV-N and SMV-G7. Insertion of the uidA gene or the green fluorescent protein gene into either modified cDNA and bombardment into primary leaves resulted in systemic expression that reflected the pattern of viral movement into uninoculated leaves. Insertion of avrB blocked symptom development and detectable viral movement in cv. Harosoy, which carries the Rpg1-b resistance gene corresponding to avrB, but not in cvs. Keburi or Hurrelbrink, which lack Rpg1-b. In Keburi and Hurrelbrink, symptoms caused by SMV carrying avrB appeared more quickly and were more severe than those caused by the virus without avrB. Insertion of avrPto enhanced symptoms in Harosoy, Hurrelbrink, and Keburi. This result was unexpected because avrPto was reported to confer avirulence on P. syringae pv. glycinea inoculated to Harosoy. We inoculated Harosoy with P. syringae pv. glycinea expressing avrPto, but observed no hypersensitive reaction, avrPto-dependent induction of pathogenesis-related protein 1a, or limitation of bacterial population growth. In Hurrelbrink, avrPto enhanced bacterial multiplication and exacerbated symptoms. Our results establish SMV as an expression vector for soybean. They demonstrate that resistance triggered by avrB is effective against SMV, and that avrB and avrPto have general virulence effects in soybean. The results also led to a reevaluation of the reported avirulence activity of avrPto in this plant.

Viral movement-cellular protein interaction

2021 ◽  
pp. 59-109
Author(s):  
Neelam Yadav ◽  
Dinesh Kumar Yadav ◽  
Sarika Yadav ◽  
S.M. Paul Khurana
Keyword(s):  
Protein Interaction ◽  
Cellular Protein ◽  
Viral Movement

scholarly journals Two Plant–Viral Movement Proteins Traffic in the Endocytic Recycling Pathway

2004 ◽  
Vol 17 (1) ◽  
pp. 164-181 ◽  
Author(s):  
Sophie Haupt ◽  
Graham H. Cowan ◽  
Angelika Ziegler ◽  
Alison G. Roberts ◽  
Karl J. Oparka ◽  
...  
Keyword(s):  
Endocytic Recycling ◽  
Movement Proteins ◽  
Viral Movement ◽  
Recycling Pathway

scholarly journals A Viral Movement Protein as a Nuclear Shuttle (The Geminivirus BR1 Movement Protein Contains Domains Essential for Interaction with BL1 and Nuclear Localization)

1996 ◽  
Vol 110 (1) ◽  
pp. 23-33 ◽  
Author(s):  
A. A. Sanderfoot ◽  
D. J. Ingham ◽  
S. G. Lazarowitz
Keyword(s):  
Nuclear Localization ◽  
Movement Protein ◽  
Viral Movement Protein ◽  
Viral Movement

scholarly journals Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

2001 ◽  
Vol 75 (17) ◽  
pp. 8045-8053 ◽  
Author(s):  
Hideaki Nagano ◽  
Kazuyuki Mise ◽  
Iwao Furusawa ◽  
Tetsuro Okuno
Keyword(s):  
Amino Acids ◽  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Cell Movement ◽  
Plant Viruses ◽  
Brome Mosaic Virus ◽  
Viral Movement ◽  
Intercellular Movement

ABSTRACT Plant viruses have movement protein (MP) gene(s) essential for cell-to-cell movement in hosts. Cucumber mosaic virus (CMV) requires its own coat protein (CP) in addition to the MP for intercellular movement. Our present results using variants of both CMV and a chimeric Brome mosaic virus with the CMV MP gene revealed that CMV MP truncated in its C-terminal 33 amino acids has the ability to mediate viral movement independently of CP. Coexpression of the intact and truncated CMV MPs extremely reduced movement of the chimeric viruses, suggesting that these heterogeneous CMV MPs function antagonistically. Sequential deletion analyses of the CMV MP revealed that the dispensability of CP occurred when the C-terminal deletion ranged between 31 and 36 amino acids and that shorter deletion impaired the ability of the MP to promote viral movement. This is the first report that a region of MP determines the requirement of CP in cell-to-cell movement of a plant virus.

scholarly journals Virus genomes reveal the factors that spread and sustained the West African Ebola epidemic

2016 ◽  
Author(s):  
Gytis Dudas ◽  
Luiz Max Carvalho ◽  
Trevor Bedford ◽  
Andrew J. Tatem ◽  
Guy Baele ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Sequence Data ◽  
Virus Disease ◽  
West African ◽  
Cross Border ◽  
Viral Movement ◽  
History Of ◽  
Virus Genomes ◽  
History Of Migration ◽  
Entire Dataset

SummaryThe 2013-2016 epidemic of Ebola virus disease in West Africa was of unprecedented magnitude, duration and impact. Extensive collaborative sequencing projects have produced a large collection of over 1600 Ebola virus genomes, representing over 5% of known cases, unmatched for any single human epidemic. In this comprehensive analysis of this entire dataset, we reconstruct in detail the history of migration, proliferation and decline of Ebola virus throughout the region. We test the association of geography, climate, administrative boundaries, demography and culture with viral movement among 56 administrative regions. Our results show that during the outbreak viral lineages moved according to a classic ‘gravity’ model, with more intense migration between larger and more proximate population centers. Notably, we find that despite a strong attenuation of international dispersal after border closures, localized cross-border transmission beforehand had already set the seeds for an international epidemic, rendering these measures relatively ineffective in curbing the epidemic. We use this empirical evidence to address why the epidemic did not spread into neighboring countries, showing that although these regions were susceptible to developing significant outbreaks, they were also at lower risk of viral introductions. Finally, viral genome sequence data uniquely reveals this large epidemic to be a heterogeneous and spatially dissociated collection of transmission clusters of varying size, duration and connectivity. These insights will help inform approaches to intervention in such epidemics in the future.

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