3D Decellularized Native Extracellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells (hWJ MSCs)

Abstract Background Angiogenesis is required in many physiological conditions, including bone regeneration, wound healing, and tissue regeneration. Cell-derived extracellular matrix (CD-ECM) could guide intricate cellular and tissue processes such as homeostasis, healing and regeneration. Methods The purpose of this study is to explore the effect and mechanism of ECM derived from decellularized Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) on endothelial cell viability and angiogenesis. Results In this study, we found for the first time that WJ-MSCs ECM could improve the angiogenesis ability of human umbilical vein endothelial cells (HUVECs) with a time-dependent manner in vitro. Mechanically, WJ-MSCs ECM activated the focal adhesion kinase (FAK)/P38 signaling pathway via integrin αVβ3, which further promoted the expression of the cellular (c)-Myc. Further, c-Myc increased histone acetylation levels of the vascular endothelial growth factor (VEGF) promoter by recruiting P300, which ultimately promoting VEGF expression. Conclusions Extracellular matrix derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF. This study is expected to provide a new approach to promote angiogenesis in bone and tissue regeneration.

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Human umbilical cord Wharton's jelly mesenchymal stem cells combined with an acellular cartilage extracellular matrix scaffold improve cartilage repair compared with microfracture in a caprine model

Osteoarthritis and Cartilage ◽

10.1016/j.joca.2018.01.019 ◽

2018 ◽

Vol 26 (7) ◽

pp. 954-965 ◽

Cited By ~ 12

Author(s):

Y. Zhang ◽

S. Liu ◽

W. Guo ◽

M. Wang ◽

C. Hao ◽

...

Keyword(s):

Stem Cells ◽

Extracellular Matrix ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Cartilage Repair ◽

Wharton’S Jelly ◽

Human Umbilical Cord ◽

Wharton's Jelly ◽

Cartilage Extracellular Matrix ◽

Extracellular Matrix Scaffold

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Evaluation of Culture Morphology of Neuronally Transdifferentiated Wharton’s Jelly Derived Mesenchymal Stem Cells

Indian Journal of Animal Research ◽

10.18805/ijar.b-4365 ◽

2021 ◽

Author(s):

T.R. Sreekumar ◽

S. Eswari ◽

K. Vijayarani

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Morphological Changes ◽

Adult Stem Cell ◽

Wharton’S Jelly ◽

Wharton's Jelly ◽

Stem Cell Source ◽

Culture Morphology ◽

One Step

Background: The prospect of mesenchymal stem cells (MSCs) as an adult stem cell source for neuronal tissue regeneration via their ability to differentiate into neurons has generated considerable excitement in regenerative cell therapy.Methods: In this study, we isolated ovine Wharton’s jelly derived MSCs and expanded in vitro in adherent culture. After the characterisation of MSCs using specific markers, we analysed the culture morphology of MSCs differentiated into neurons by a two-step chemical-based induction protocols involving a pre-induction step and a direct one step chemical-based induction protocol. Morphological changes after induction were evaluated.Result: In both the methods, after neuronal induction, the cells displayed phenotypic characteristic of neurons and comparatively less cytotoxicity was observed in the direct induction method. This study confirmed the possibility of generating neuron like cells from ovine WJ-MSCs and thereby exploring the potential of MSCs as therapeutic tool for treating neurological disorders in Veterinary Medicine.

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AFM-based Analysis of Wharton’s Jelly Mesenchymal Stem Cells

International Journal of Molecular Sciences ◽

10.3390/ijms20184351 ◽

2019 ◽

Vol 20 (18) ◽

pp. 4351

Author(s):

Renata Szydlak ◽

Marcin Majka ◽

Małgorzata Lekka ◽

Marta Kot ◽

Piotr Laidler

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Young’S Modulus ◽

Young's Modulus ◽

Wharton’S Jelly ◽

In Vitro Cultivation ◽

Multipotent Stem Cells ◽

Wharton's Jelly ◽

Migration Potential

Wharton’s jelly mesenchymal stem cells (WJ-MSCs) are multipotent stem cells that can be used in regenerative medicine. However, to reach the high therapeutic efficacy of WJ-MSCs, it is necessary to obtain a large amount of MSCs, which requires their extensive in vitro culturing. Numerous studies have shown that in vitro expansion of MSCs can lead to changes in cell behavior; cells lose their ability to proliferate, differentiate and migrate. One of the important measures of cells’ migration potential is their elasticity, determined by atomic force microscopy (AFM) and quantified by Young’s modulus. This work describes the elasticity of WJ-MSCs during in vitro cultivation. To identify the properties that enable transmigration, the deformability of WJ-MSCs that were able to migrate across the endothelial monolayer or Matrigel was analyzed by AFM. We showed that WJ-MSCs displayed differences in deformability during in vitro cultivation. This phenomenon seems to be strongly correlated with the organization of F-actin and reflects the changes characteristic for stem cell maturation. Furthermore, the results confirm the relationship between the deformability of WJ-MSCs and their migration potential and suggest the use of Young’s modulus as one of the measures of competency of MSCs with respect to their possible use in therapy.

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