Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue

2018 ◽  
pp. 37-92 ◽  
Author(s):  
Meenakshi Gaur ◽  
Marek Dobke ◽  
Victoria V. Lunyak
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells

Chondrogenic potential of bone marrow- and adipose tissue-derived adult human mesenchymal stem cells

2010 ◽  
Vol 20 (3-4) ◽  
pp. 145-158 ◽  
Author(s):  
M.C. Ronzière ◽  
E. Perrier ◽  
F. Mallein-Gerin ◽  
A.-M. Freyria
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Adult Human ◽  
Chondrogenic Potential

Human Mesenchymal Stem Cells from Adipose Tissue Differentiated into Neuronal or Glial Phenotype Express Different Aquaporins

2016 ◽  
Vol 54 (10) ◽  
pp. 8308-8320 ◽  
Author(s):  
Rosanna Avola ◽  
Adriana Carol Eleonora Graziano ◽  
Giovanna Pannuzzo ◽  
Venera Cardile
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells

scholarly journals Regulation of CXCR6 Expression on Adipocytes and Osteoblasts Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Seung-Cheol Lee ◽  
Yoo-Jung Lee ◽  
Min Kyoung Shin ◽  
Jung-Suk Sung
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Activation ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Human Mesenchymal Stem Cells ◽  
De Novo Synthesis ◽  
Differentiation Potential ◽  
Differentiated Cells

Human mesenchymal stem cells derived from adipose tissue (hADMSCs) are a desirable candidate in regenerative medicine. hADMSCs secrete growth factors, cytokines, and chemokines and also express various receptors that are important in cell activation, differentiation, and migration to injured tissue. We showed that the expression level of chemokine receptor CXCR6 was significantly increased by ~2.5-fold in adipogenic-differentiated cells (Ad), but not in osteogenic-differentiated cells (Os) when compared with hADMSCs. However, regulation of CXCR6 expression on hADMSCs by using lentiviral particles did not affect the differentiation potential of hADMSCs. Increased expression of CXCR6 on Ad was mediated by both receptor recycling, which was in turn regulated by secretion of CXCL16, and de novo synthesis. The level of soluble CXCL16 was highly increased in both Ad and Os in particular, which inversely correlates with the expression on a transmembrane-bound form of CXCL16 that is cleaved by disintegrin and metalloproteinase. We concluded that the expression of CXCR6 is regulated by receptor degradation or recycling when it is internalized by interaction with CXCL16 and by de novo synthesis of CXCR6. Overall, our study may provide an insight into the molecular mechanisms of the CXCR6 reciprocally expressed on differentiated cells from hADMSCs.

scholarly journals Isolation and Differentiation Potential of Human Mesenchymal Stem Cells From Adipose Tissue Harvested by Water Jet-Assisted Liposuction

2015 ◽  
Vol 35 (8) ◽  
pp. 1030-1039 ◽  
Author(s):  
Juliane Meyer ◽  
Achim Salamon ◽  
Nicole Herzmann ◽  
Stefanie Adam ◽  
Hans-Dieter Kleine ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Water Jet ◽  
Differentiation Potential

scholarly journals Adipose Tissue–Derived Human Mesenchymal Stem Cells Mediated Prodrug Cancer Gene Therapy

2007 ◽  
Vol 67 (13) ◽  
pp. 6304-6313 ◽  
Author(s):  
Lucia Kucerova ◽  
Veronika Altanerova ◽  
Miroslava Matuskova ◽  
Silvia Tyciakova ◽  
Cestmir Altaner
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Cancer Gene Therapy ◽  
Cancer Gene
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