scholarly journals Adipogenic differentiation of adipose tissue-derived human mesenchymal stem cells: effect of gastric bypass surgery

2012 ◽  
Vol 26 (12) ◽  
pp. 3449-3456 ◽  
Author(s):  
Jie-Gen Chen ◽  
Anna Spagnoli ◽  
Alfonso Torquati
Keyword(s):  
Stem Cells ◽  
Gastric Bypass ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Bypass Surgery ◽  
Gastric Bypass Surgery ◽  
Adipogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals Calcitriol combined with calcium chloride causes apoptosis in undifferentiated adipose tissue-derived human mesenchymal stem cells, but this effect decreases during adipogenic differentiation

2018 ◽  
Vol 108 ◽  
pp. 914-924 ◽  
Author(s):  
João Renato Pesarini ◽  
Edwin José Torres de Oliveira ◽  
Lucas Roberto Pessatto ◽  
Ana Paula Maluf Rabacow ◽  
Melissa Camassola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Calcium Chloride ◽  
Adipogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals A polyion complex sensor array for markerless and noninvasive identification of differentiated mesenchymal stem cells from human adipose tissue

2015 ◽  
Vol 6 (10) ◽  
pp. 5831-5836 ◽  
Author(s):  
Shunsuke Tomita ◽  
Miho Sakao ◽  
Ryoji Kurita ◽  
Osamu Niwa ◽  
Keitaro Yoshimoto
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Sensor Array ◽  
Adipogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Human Adipose Tissue ◽  
Polyion Complex ◽  
Polyion Complexes

A sensor array of cross-reactive polyion complexes enabled markerless and noninvasive identification of osteogenic and adipogenic differentiation of human mesenchymal stem cells.

scholarly journals The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1831
Author(s):  
Thitianan Kulsirirat ◽  
Sittisak Honsawek ◽  
Mariko Takeda-Morishita ◽  
Nuttanan Sinchaipanid ◽  
Wanvisa Udomsinprasert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Fat Pad ◽  
Alizarin Red ◽  
Lineage Differentiation ◽  
Expression Of Genes ◽  
Dose Dependent ◽  
Oil Red O Staining

Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of Andrographis paniculata, and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the present study, our goal was to evaluate the innovative effects of andrographolide on viability and Tri-lineage differentiation of human mesenchymal stem cells from suprapatellar fat pad tissues. The results revealed that andrographolide had no cytotoxic effects when the concentration was less than 12.5 µM. Interestingly, andrographolide had significantly enhanced, dose dependent, osteogenesis and chondrogenesis as evidenced by a significantly intensified stain for Alizarin Red S, Toluidine Blue and Alcian Blue. Moreover, andrographolide can upregulate the expression of genes related to osteogenic and chondrogenic differentiation, including Runx2, OPN, Sox9, and Aggrecan in mesenchymal stem cells from human suprapatellar fat pad tissues. In contrast, andrographolide suppressed adipogenic differentiation as evidenced by significantly diminished Oil Red O staining and expression levels for adipogenic-specific genes for PPAR-γ2 and LPL. These findings confirm that andrographolide can specifically enhance osteogenesis and chondrogenesis of mesenchymal stem cells from human suprapatellar fat pad tissues. It has potential as a therapeutic agent derived from natural sources for regenerative medicine.

scholarly journals MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase

2019 ◽  
Vol 60 (12) ◽  
pp. 1187
Author(s):  
Yuting Wang ◽  
Lixin Lin ◽  
Yong Huang ◽  
Junjun Sun ◽  
Xueming Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Adipogenic Differentiation ◽  
Human Adipose Tissue

Chondrogenic potential of bone marrow- and adipose tissue-derived adult human mesenchymal stem cells

2010 ◽  
Vol 20 (3-4) ◽  
pp. 145-158 ◽  
Author(s):  
M.C. Ronzière ◽  
E. Perrier ◽  
F. Mallein-Gerin ◽  
A.-M. Freyria
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Adult Human ◽  
Chondrogenic Potential

Human Mesenchymal Stem Cells from Adipose Tissue Differentiated into Neuronal or Glial Phenotype Express Different Aquaporins

2016 ◽  
Vol 54 (10) ◽  
pp. 8308-8320 ◽  
Author(s):  
Rosanna Avola ◽  
Adriana Carol Eleonora Graziano ◽  
Giovanna Pannuzzo ◽  
Venera Cardile
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells

scholarly journals Regulation of CXCR6 Expression on Adipocytes and Osteoblasts Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Seung-Cheol Lee ◽  
Yoo-Jung Lee ◽  
Min Kyoung Shin ◽  
Jung-Suk Sung
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Activation ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Human Mesenchymal Stem Cells ◽  
De Novo Synthesis ◽  
Differentiation Potential ◽  
Differentiated Cells

Human mesenchymal stem cells derived from adipose tissue (hADMSCs) are a desirable candidate in regenerative medicine. hADMSCs secrete growth factors, cytokines, and chemokines and also express various receptors that are important in cell activation, differentiation, and migration to injured tissue. We showed that the expression level of chemokine receptor CXCR6 was significantly increased by ~2.5-fold in adipogenic-differentiated cells (Ad), but not in osteogenic-differentiated cells (Os) when compared with hADMSCs. However, regulation of CXCR6 expression on hADMSCs by using lentiviral particles did not affect the differentiation potential of hADMSCs. Increased expression of CXCR6 on Ad was mediated by both receptor recycling, which was in turn regulated by secretion of CXCL16, and de novo synthesis. The level of soluble CXCL16 was highly increased in both Ad and Os in particular, which inversely correlates with the expression on a transmembrane-bound form of CXCL16 that is cleaved by disintegrin and metalloproteinase. We concluded that the expression of CXCR6 is regulated by receptor degradation or recycling when it is internalized by interaction with CXCL16 and by de novo synthesis of CXCR6. Overall, our study may provide an insight into the molecular mechanisms of the CXCR6 reciprocally expressed on differentiated cells from hADMSCs.

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