Creating Unique Cell Microenvironments for the Engineering of a Functional Cardiac Patch

2010 ◽  
pp. 81-94
Author(s):  
Tal Dvir ◽  
Jonathan Leor ◽  
Smadar Cohen
Keyword(s):  
Unique Cell ◽  
Cardiac Patch

scholarly journals The Telocytes in the Subepicardial Niche

2019 ◽  
Vol 9 (8) ◽  
pp. 1615 ◽  
Author(s):  
Cristian Bogdan Iancu ◽  
Mugurel Constantin Rusu ◽  
Laurenţiu Mogoantă ◽  
Sorin Hostiuc ◽  
Oana Daniela Toader
Keyword(s):  
Stromal Cells ◽  
Lymphatic Vessels ◽  
Growth Factor Receptor ◽  
Immunohistochemical Method ◽  
Lymphatic Endothelial Cells ◽  
Cell Type ◽  
Ki 67 ◽  
Epicardial Cells ◽  
Immunohistochemical Evidence ◽  
Unique Cell

A great interest has developed over the last several years in research on interstitial Cajal-like cells (ICLCs), later renamed to telocytes (TCs). Such studies are restricted by diverse limitations. We aimed to critically review (sub)epicardial ICLCs/TCs and to bring forward supplemental immunohistochemical evidence on (sub)epicardial stromal niche inhabitants. We tested the epicardial expressions of CD117/c-kit, CD34, Cytokeratin 7 (CK7), Ki67, Platelet-Derived Growth Factor Receptor (PDGFR)-α and D2-40 in adult human cardiac samples. The mesothelial epicardial cells expressed D2-40, CK7, CD117/c-kit and PDGFR-α. Subepicardial D2-40-positive lymphatic vessels and isolated D2-40-positive and CK7-positive subepicardial cells were also found. Immediate submesothelial spindle-shaped cells expressed Ki-67. Submesothelial stromal cells and endothelial tubes were PDGFR-α-positive and CD34-positive. The expression of CD34 was pan-stromal, so a particular stromal cell type could not be distinguished. The stromal expression of CD117/c-kit was also noted. It seems that epicardial TCs could not be regarded as belonging to a unique cell type until (pre)lymphatic endothelial cells are inadequately excluded. Markers such as CD117/c-kit or CD34 seem to be improper for identifying TCs as a distinctive cell type. Care should be taken when using the immunohistochemical method and histological interpretations, as they may not produce accurate results.

scholarly journals Plasma Polymerized Allylamine—The Unique Cell-Attractive Nanolayer for Dental Implant Materials

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1004 ◽  
Author(s):  
J. Barbara Nebe ◽  
Henrike Rebl ◽  
Michael Schlosser ◽  
Susanne Staehlke ◽  
Martina Gruening ◽  
...  
Keyword(s):  
Intracellular Signaling ◽  
Microwave Plasma ◽  
Cell Attachment ◽  
Tetragonal Zirconia ◽  
Surrounding Tissue ◽  
Material Surface ◽  
Cell Physiology ◽  
Ceramic Surface ◽  
Plasma Chemical ◽  
Unique Cell

Biomaterials should be bioactive in stimulating the surrounding tissue to accelerate the ingrowth of permanent implants. Chemical and topographical features of the biomaterial surface affect cell physiology at the interface. A frequently asked question is whether the chemistry or the topography dominates the cell-material interaction. Recently, we demonstrated that a plasma-chemical modification using allylamine as a precursor was able to boost not only cell attachment and cell migration, but also intracellular signaling in vital cells. This microwave plasma process generated a homogenous nanolayer with randomly distributed, positively charged amino groups. In contrast, the surface of the human osteoblast is negatively charged at −15 mV due to its hyaluronan coat. As a consequence, we assumed that positive charges at the material surface—provoking electrostatic interaction forces—are attractive for the first cell encounter. This plasma-chemical nanocoating can be used for several biomaterials in orthopedic and dental implantology like titanium, titanium alloys, calcium phosphate scaffolds, and polylactide fiber meshes produced by electrospinning. In this regard, we wanted to ascertain whether plasma polymerized allylamine (PPAAm) is also suitable for increasing the attractiveness of a ceramic surface for dental implants using Yttria-stabilized tetragonal zirconia.

Bioengineered cardiac patch constructed from multilayered mesenchymal stem cells for myocardial repair

Biomaterials ◽  
2008 ◽  
Vol 29 (26) ◽  
pp. 3547-3556 ◽  
Author(s):  
Hao-Ji Wei ◽  
Chun-Hung Chen ◽  
Wen-Yu Lee ◽  
Iwen Chiu ◽  
Shiaw-Min Hwang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Myocardial Repair ◽  
Cardiac Patch

scholarly journals Evidence for the presence of stem cell-like progenitor cells in human adult pancreas

2007 ◽  
Vol 195 (3) ◽  
pp. 407-414 ◽  
Author(s):  
Min Zhao ◽  
Stephanie A Amiel ◽  
Michael R Christie ◽  
Paolo Muiesan ◽  
Parthi Srinivasan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Adult Life ◽  
Stem Cell Markers ◽  
Human Pancreas ◽  
Β Cells ◽  
Adult Human ◽  
Pancreatic Cells ◽  
Exocrine Cell ◽  
Cell Fractions ◽  
Unique Cell

The origin of cells replacing ageing β-cells in adult life is unknown. This study assessed the expression of classic stem cell markers: Oct4, Sox2 and CD34 in islet-enriched fractions versus exocrine cell-enriched fractions from 25 adult human pancreases following human islet isolation. Expression of Oct4, Sox2 and CD34 mRNAs was found in all cell samples, with no significant differences between endocrine and exocrine cell fractions. Immunohistochemical staining for Oct4, Sox2, CD133, CD34, CK19, insulin and nestin on human pancreas sections showed that the majority of Oct4+ve cells were found in the walls of small ducts. Similar localisations were observed for Sox2+ve cells. The majority of Sox2+ve cells were found to co-express Oct4 proteins, but not vice versa. Cells positive for Oct4 and Sox2 appeared to be a unique cell population in the adult human pancreases without co-expression for CK19, CD34, CD133, insulin and nestin proteins. The numbers of Oct4+ve and Sox2+ve cells varied among donors and were ∼1–200 and 1–30 per 100 000 pancreatic cells respectively.

scholarly journals Recent fabrications and applications of cardiac patch in myocardial infarction treatment

View ◽  
2021 ◽  
pp. 20200153
Author(s):  
Mei Li ◽  
Hao Wu ◽  
Yuehui Yuan ◽  
Benhui Hu ◽  
Ning Gu
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Patch

Thermal Characterizacion of Konjac/Gellam Gum Hydrogels for Cardiac Patch

2021 ◽  
Author(s):  
David Patrocinio Caballero ◽  
Jorge Loureiro ◽  
Victor P. Galvan Chacon ◽  
Maximiano P. Ribeiro ◽  
Sonia Miguel ◽  
...  
Keyword(s):  
Cardiac Patch

scholarly journals A unique cell division machinery in the Archaea

2008 ◽  
Vol 105 (48) ◽  
pp. 18942-18946 ◽  
Author(s):  
A.-C. Lindas ◽  
E. A. Karlsson ◽  
M. T. Lindgren ◽  
T. J. G. Ettema ◽  
R. Bernander
Keyword(s):  
Cell Division ◽  
Unique Cell

scholarly journals A Regenerative Cardiac Patch Formed by Spray Painting of Biomaterials onto the Heart

2017 ◽  
Vol 23 (3) ◽  
pp. 146-155 ◽  
Author(s):  
Junnan Tang ◽  
Adam Vandergriff ◽  
Zegen Wang ◽  
Michael Taylor Hensley ◽  
Jhon Cores ◽  
...  
Keyword(s):  
Spray Painting ◽  
Cardiac Patch

Examining the Coiling Motion of Soft Actuators Reinforced With Tilted Helix Fibers

2018 ◽  
Author(s):  
Ryan Geer ◽  
Suyi Li
Keyword(s):  
Longitudinal Axis ◽  
Robotic Manipulation ◽  
Proof Of Concept ◽  
Soft Actuator ◽  
Kevlar Fiber ◽  
New Family ◽  
End Caps ◽  
Soft Actuators ◽  
Unique Cell ◽  
Cell Wall Architecture

This study aims to examine the coiling and uncoiling motion of a soft pneumatic actuator reinforced with tilted helix fibers. Coiling motion can be quite useful for robotic manipulation and locomotion purposes. This research proposes and investigates a novel actuator that is inspired and derived from the unique cell wall architecture in the seed appendage of Stork’s Bill plant (Erodium Gruinum). These plant cells are reinforced by cellulose fibers distributed in a tilted helix pattern — helixes that are tilted at a certain angle with respect to the longitudinal axis of the cell. As a result, the seed appendage can coil and uncoil via a combination of twisting and bending. This paper discusses the design, fabrication, and testing of a soft actuator that can mimic this sophisticated motion. This actuator consists of Kevlar fiber thread wrapped around a silicon rubber body that has the shape of a tube. The tube will be capped at both ends so that it can be pressurized internally to induce motion. Once the design parameter has been chosen, the soft actuator are fabricated by 1) designing and 3D printing molds, 2) tube casting and fiber wrapping, and 3) creating the end caps for pressure sealing. Carefully executing these fabrication steps is essential because any errors could give undesired deformation. Several soft actuators prototypes are fabricated based on different design choices regarding the actuator radius, tube wall thickness, and the number of tilted helix fibers (aka. fiber coverage). Proof-of-concept tests show that these actuator prototypes can indeed exhibit a combined twisting and bending under internal pressurization: all are the necessary receipts to achieve the coiling and uncoiling motion. Result of this paper can pave the way for a new family of soft actuators capable of unprecedented and sophisticated actuation motions, which are particularly appealing for soft robot application.

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