In Silico and In Vitro Analysis of Small Breast Epithelial Mucin as a Marker for Bone Marrow Micrometastasis in Breast Cancer

2008 ◽  
pp. 331-339 ◽  
Author(s):  
Manuel Valladares Ayerbes ◽  
Silvia Díaz-Prado ◽  
Daniel Ayude ◽  
Rosario G. Campelo ◽  
Pilar Iglesias ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
In Silico ◽  
Small Breast ◽  
Bone Marrow Micrometastasis ◽  
Small Breast Epithelial Mucin ◽  
Breast Epithelial ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Diagnostic accuracy of small breast epithelial mucin mRNA as a marker for bone marrow micrometastasis in breast cancer: a pilot study

2009 ◽  
Vol 135 (9) ◽  
pp. 1185-1195 ◽  
Author(s):  
Manuel Valladares-Ayerbes ◽  
Pilar Iglesias-Díaz ◽  
Silvia Díaz-Prado ◽  
Daniel Ayude ◽  
Vanessa Medina ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Pilot Study ◽  
Diagnostic Accuracy ◽  
Small Breast ◽  
Bone Marrow Micrometastasis ◽  
Small Breast Epithelial Mucin ◽  
Breast Epithelial

Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

2018 ◽  
Vol 18 ◽  
Author(s):  
Chaitra Venugopal ◽  
Christopher Shamir ◽  
Sivapriya Senthilkumar ◽  
Janitri Venkatachala Babu ◽  
Peedikayil Kurien Sonu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuroprotective Effects ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Rat Bone

scholarly journals In vitro analysis of integrin expression in stem cells from bone marrow and cord blood during chondrogenic differentiation

2009 ◽  
Vol 13 (6) ◽  
pp. 1175-1184 ◽  
Author(s):  
Ulrich Reinhart Goessler ◽  
Peter Bugert ◽  
Karen Bieback ◽  
Jens Stern-Straeter ◽  
Gregor Bran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cord Blood ◽  
Chondrogenic Differentiation ◽  
Integrin Expression ◽  
Vitro Analysis ◽  
In Vitro Analysis

In silico Repurposing of Anticancer Drug (5-Fluorouracil) as an Antibacterial Agent against Klebsiella pneumoniae

2021 ◽  
Vol 18 ◽  
Author(s):  
Amey Sharma ◽  
Apoorva Rana ◽  
Lakshya Mangtani ◽  
Aakanksha Kalra ◽  
Ravi Ranjan Kumar Niraj
Keyword(s):  
Molecular Docking ◽  
Klebsiella Pneumoniae ◽  
Thymidylate Synthase ◽  
In Silico ◽  
Homology Modelling ◽  
Klebsiella Pneumonia ◽  
Drug Resistant ◽  
Vitro Analysis ◽  
In Vitro Analysis

Background: Infections caused by drug resistant microorganisms have been increasing worldwide thereby being one of the major causes of morbidity in the 21st century. Klebsiella pneumoniae is one such bacteria causing lung inflammation, lung injury and death. Emergence of hyper-virulent and drug resistant species such as ESBL and CRKP has made this microbe a serious and urgent threat. The pace of emergence of these species is outgrowing the development of novel drug and vaccine candidates thereby focusing on drug repurposing approach. Objective: 1. Homology Modelling of Thymidylate Synthase. 2. Verification of Modelled Structure. 3. Molecular Docking. 4. Molecular Dynamic Simulation of Docked Complex. 5. In vitro analysis of 5-FU activity against Klebsiella pneumonia. Method: The 3-D structure of Thymidylate Synthase was predicted using Swiss-Model server and validated by in silico approaches. - Determination protein-protein interactions using STRING database. - Molecular docking. - MD simulations of 5-FU with predicted structure of thymidylate synthase. - In vitro antimicrobial drug sensitivity assay at different concentrations. Result: Hydrogen bond was observed in Molecular Docking - Protein-ligand complex remains stable during simulation. - 5-FU shows antimicrobial activity against Klebsiella pneumonia during In vitro study. Conclusion: Both In silico as well as in vitro analysis have indicated that 5-FU can potentially be developed as an antimicrobial agent towards Klebsiella pneumonia

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