Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches

2020 ◽  
pp. 7-27
Author(s):  
Adam B. Cornwell ◽  
Andrew V. Samuelson
Keyword(s):  
High Throughput ◽  
C Elegans

scholarly journals High-throughput behavioral screen in C. elegans reveals Parkinson’s disease drug candidates

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Salman Sohrabi ◽  
Danielle E. Mor ◽  
Rachel Kaletsky ◽  
William Keyes ◽  
Coleen T. Murphy
Keyword(s):  
Neural Network ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
High Throughput ◽  
Potential Candidate ◽  
Automated Scoring ◽  
C Elegans ◽  
Drug Candidates ◽  
Branched Chain ◽  
Approved Drugs

AbstractWe recently linked branched-chain amino acid transferase 1 (BCAT1) dysfunction with the movement disorder Parkinson’s disease (PD), and found that RNAi-mediated knockdown of neuronal bcat-1 in C. elegans causes abnormal spasm-like ‘curling’ behavior with age. Here we report the development of a machine learning-based workflow and its application to the discovery of potentially new therapeutics for PD. In addition to simplifying quantification and maintaining a low data overhead, our simple segment-train-quantify platform enables fully automated scoring of image stills upon training of a convolutional neural network. We have trained a highly reliable neural network for the detection and classification of worm postures in order to carry out high-throughput curling analysis without the need for user intervention or post-inspection. In a proof-of-concept screen of 50 FDA-approved drugs, enasidenib, ethosuximide, metformin, and nitisinone were identified as candidates for potential late-in-life intervention in PD. These findings point to the utility of our high-throughput platform for automated scoring of worm postures and in particular, the discovery of potential candidate treatments for PD.

scholarly journals High‐Throughput Screening for Novel Anti‐Infectives Using a C. elegans Pathogenesis Model

2014 ◽  
Vol 6 (1) ◽  
pp. 25-37 ◽  
Author(s):  
Annie L. Conery ◽  
Jonah Larkins‐Ford ◽  
Frederick M. Ausubel ◽  
Natalia V. Kirienko
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
C Elegans

scholarly journals Laterally Orienting C. elegans Using Geometry at Microscale for High-Throughput Visual Screens in Neurodegeneration and Neuronal Development Studies

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e35037 ◽  
Author(s):  
Ivan de Carlos Cáceres ◽  
Nicholas Valmas ◽  
Massimo A. Hilliard ◽  
Hang Lu
Keyword(s):  
High Throughput ◽  
Neuronal Development ◽  
Development Studies ◽  
C Elegans

scholarly journals CAENORHABDITIS ELEGANS AS A MODEL OF AIR POLLUTION TOXICITY DURING DEVELOPMENT AND LIFESPAN

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S97-S97
Author(s):  
Amin Haghani ◽  
Hans M Dalton ◽  
Nikoo Safi ◽  
Farimah Shirmohammadi ◽  
Constantinos Sioutas ◽  
...  
Keyword(s):  
Air Pollution ◽  
Caenorhabditis Elegans ◽  
Mouse Models ◽  
High Throughput ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Exposure Model ◽  
C Elegans ◽  
Biological Responses ◽  
Short Term Exposure

Abstract Air pollution (AirPoll) is among the leading human mortality risk factors and yet little is known about the molecular mechanisms of this global environmental toxin. Our recent studies using mouse models even showed genetic variation and sex can alter biological responses to air pollution. To expand genetic studies of AirPoll toxicity throughout the lifespan, we introduced Caenorhabditis elegans as a new AirPoll exposure model which has a short lifespan, high throughput capabilities and shared longevity pathways with mammals. Acute exposure of C. elegans to airborne nanosized AirPoll matter (nPM) caused similar gene expression changes to our prior findings in cell culture and mouse models. Initial C. elegans responses to nPM included antioxidant, inflammatory and Alzheimer homolog genes. The magnitude of changes was dependent on the developmental stage of the worms. Even short term exposure of C. elegans to nPM altered developmental and lifespan hormetic effects, with pathways that included skn-1/Nrf family antioxidant responses. We propose C. elegans as a new and complementary model for mouse and cultured cells to study AirPoll across the lifespan. Future chronic nPM exposure and high throughput genetic screening of C. elegans can identify other major regulators of the developmental and lifespan effects of air pollution. This work was supported by grants R01AG051521 (CEF); R21AG05020 (CEF); Cure Alzheimer’s Fund (CEF); R01GM109028 (SPC), F31AG051382 (HMD) and T32AG000037 (HMD), T32AG052374 (AH).

scholarly journals High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

10.3791/2745 ◽  
2011 ◽  
Author(s):  
Chi K. Leung ◽  
Andrew Deonarine ◽  
Kevin Strange ◽  
Keith P. Choe
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
C Elegans

scholarly journals High-throughput, motility-based sorter for microswimmers such as C. elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (13) ◽  
pp. 2790-2798 ◽  
Author(s):  
Jinzhou Yuan ◽  
Jessie Zhou ◽  
David M. Raizen ◽  
Haim H. Bau
Keyword(s):  
High Throughput ◽  
C Elegans

This paper describes the design, fabrication, characterization, and applications of a high-throughput motility-based sorter for microswimmers such asC. elegans.

scholarly journals High-throughput screening in the C. elegans nervous system

2017 ◽  
Vol 80 ◽  
pp. 192-197 ◽  
Author(s):  
Holly E. Kinser ◽  
Zachary Pincus
Keyword(s):  
Nervous System ◽  
High Throughput ◽  
High Throughput Screening ◽  
C Elegans

scholarly journals Automated screening ofC. elegansneurodegeneration mutants enabled by microfluidics and image analysis algorithms

2018 ◽  
Vol 10 (9) ◽  
pp. 539-548 ◽  
Author(s):  
Ivan de Carlos Cáceres ◽  
Daniel A. Porto ◽  
Ivan Gallotta ◽  
Pamela Santonicola ◽  
Josue Rodríguez-Cordero ◽  
...  
Keyword(s):  
Image Analysis ◽  
Spinal Muscular Atrophy ◽  
High Throughput ◽  
Muscular Atrophy ◽  
High Throughput Screen ◽  
C Elegans ◽  
Genetic Mechanisms ◽  
Automated Screening

A fully automated high-throughput screen usingC. elegansto investigate genetic mechanisms affecting spinal muscular atrophy (SMA).

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