In Vitro Gynogenesis in Leek (Allium ampeloprasum L.)

Author(s):  
Fevziye Celebi-Toprak ◽  
Ali Ramazan Alan
Keyword(s):  
Author(s):  
Shahid Khan ◽  
Neeta Raj Sharma

Objective: In vitro analysis of Allium sativum and Allium ampeloprasum was performed to evaluate their antifungal potential against Alternaria triticina (ITCC 5496), causative agent of leaf blight in wheat and Magnaporthe oryzae (ITCC 6808), causative agent of blast disease in rice.Methods: Ethanol extracts of A. ampeloprasum and A. sativum were prepared by crushing their bulb in liquid nitrogen and then immersing them in 90% ethanol and 100% ethanol separately. The antifungal activity test was determined by quantitative assay using 96-well microtiter plate and results were statistically analyzed using GraphPad Prism v. 5.03.Results: A. triticina and M. oryzae showed above 90% and 95% growth inhibition, respectively against the ethanol extracts of A. ampeloprasum. Conversely, growth inhibition of either fungus remained mostly below 35% against ethanol extracts of A. sativum at all tested concentrations.Conclusion: Ethanol extracts of A. ampeloprasum have relatively higher antifungal potential than ethanol extracts of A. sativum and could be considered as a natural alternative to chemical fungicides.Keywords: Allium sativum, Allium ampeloprasum, Alternaria triticina, Magnaporthe oryzae.


1995 ◽  
Vol 14 (7) ◽  
pp. 423-427 ◽  
Author(s):  
B. Silvertand ◽  
E. Jacobsen ◽  
J. Mazereeuw ◽  
P. Lavrijsen ◽  
A. van Harten

1994 ◽  
Vol 74 (1) ◽  
pp. 155-158 ◽  
Author(s):  
Janet E. A. Seabrook

Garlic (Allium sativum and A. ampeloprasum) shoot cultures were established in vitro for cultivars Block, Chets, Shaw, #72 and Elephant garlic. Explants comprised a small (1–3 mm) piece of basal plate, an axillary bud and one or two subtending bulb scales. A medium containing MS salts and 0.1 mg L−1 (0.5 μM) NAA and 2.0 mg L−1 (8.8 μM) BAP or 2iP promoted axillary shoot multiplication., Bulb formation of garlic shoot cultures was obtained on a medium containing a lower concentration of macro- and minor elements. The substitution of glucose and manitol for sucrose resulted in a bulbing medium with higher carbohydrate levels than the multiplication medium. Genotypic differences for both shoot multiplication and bulb formation in vitro were observed. The mean number of shoots produced were: Chets (9.5), #72 (8.0), Elephant (7.2), Shaw (7.1) and Block (6.1). The mean numbers of bulbs per 50 shoot explants were: Chets (62), Elephant (44), #72 (43), Shaw (26) and Block (9). Key words:Allium sativum, Allium ampeloprasum, genotypic responses, micro-propagation, tissue culture


2016 ◽  
pp. 55-60
Author(s):  
A. Kaska ◽  
S. Yildirim ◽  
B. Top ◽  
F. Celebi-Toprak ◽  
A.R. Alan

1995 ◽  
Vol 40 (2) ◽  
pp. 195-196 ◽  
Author(s):  
Yasseen Mohamed-Yasseen ◽  
Sheryl A. Barringer ◽  
Walter E. Splittstoesser

Author(s):  
P.L. Moore

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.


Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.


Author(s):  
E. J. Kollar

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.


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