In vitro propagation and bulb formation of garlic
Garlic (Allium sativum and A. ampeloprasum) shoot cultures were established in vitro for cultivars Block, Chets, Shaw, #72 and Elephant garlic. Explants comprised a small (1–3 mm) piece of basal plate, an axillary bud and one or two subtending bulb scales. A medium containing MS salts and 0.1 mg L−1 (0.5 μM) NAA and 2.0 mg L−1 (8.8 μM) BAP or 2iP promoted axillary shoot multiplication., Bulb formation of garlic shoot cultures was obtained on a medium containing a lower concentration of macro- and minor elements. The substitution of glucose and manitol for sucrose resulted in a bulbing medium with higher carbohydrate levels than the multiplication medium. Genotypic differences for both shoot multiplication and bulb formation in vitro were observed. The mean number of shoots produced were: Chets (9.5), #72 (8.0), Elephant (7.2), Shaw (7.1) and Block (6.1). The mean numbers of bulbs per 50 shoot explants were: Chets (62), Elephant (44), #72 (43), Shaw (26) and Block (9). Key words:Allium sativum, Allium ampeloprasum, genotypic responses, micro-propagation, tissue culture