Functional Analysis of Actin-Binding Proteins in the Central Nervous System of

Abstract Septins are cytoskeletal GTP-binding proteins involved in processes characterized by active membrane movement, such as cytokinesis, vesicle trafficking and exocytosis. Most septins are expressed ubiquitously, however, some septins accumulate in particular tissues. The ubiquitous SEPT11 also shows high expression levels in the central nervous system and in platelets. Here, SEPT11 is involved in vesicle trafficking and may play a role in synaptic connectivity. Interestingly, mice that harbor a homozygous Sept11 null mutation, die in utero. From day 11.5 post coitum onwards, development of homozygous embryos seems to be retarded and the embryos from day 13.5 onwards were dead.

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Expression and functional analysis of nucleic acid sensors in cells of the central nervous system

Journal of Neuroimmunology ◽

10.1016/j.jneuroim.2014.08.222 ◽

2014 ◽

Vol 275 (1-2) ◽

pp. 83

Author(s):

Donal Cox ◽

Glyn Williams ◽

Neil Kearney ◽

Andrew Bowie ◽

Marina Lynch ◽

...

Keyword(s):

Central Nervous System ◽

Functional Analysis ◽

Nervous System ◽

Nucleic Acid ◽

The Central Nervous System ◽

In Cells ◽

Acid Sensors

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New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System

Brain Sciences ◽

10.3390/brainsci11050634 ◽

2021 ◽

Vol 11 (5) ◽

pp. 634

Author(s):

Krisztina Kelemen ◽

Tibor Szilágyi

Keyword(s):

Gene Expression ◽

Central Nervous System ◽

Nervous System ◽

Calcium Binding ◽

Binding Proteins ◽

Expression Profiles ◽

Calcium Binding Proteins ◽

Brain Atlas ◽

Specific Cell ◽

The Central Nervous System

Although Ca2+ ion plays an essential role in cellular physiology, calcium-binding proteins (CaBPs) were long used for mainly as immunohistochemical markers of specific cell types in different regions of the central nervous system. They are a heterogeneous and wide-ranging group of proteins. Their function was studied intensively in the last two decades and a tremendous amount of information was gathered about them. Girard et al. compiled a comprehensive list of the gene-expression profiles of the entire EF-hand gene superfamily in the murine brain. We selected from this database those CaBPs which are related to information processing and/or neuronal signalling, have a Ca2+-buffer activity, Ca2+-sensor activity, modulator of Ca2+-channel activity, or a yet unknown function. In this way we created a gene function-based selection of the CaBPs. We cross-referenced these findings with publicly available, high-quality RNA-sequencing and in situ hybridization databases (Human Protein Atlas (HPA), Brain RNA-seq database and Allen Brain Atlas integrated into the HPA) and created gene expression heat maps of the regional and cell type-specific expression levels of the selected CaBPs. This represents a useful tool to predict and investigate different expression patterns and functions of the less-known CaBPs of the central nervous system.

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