Liposomes as model cellular systems

2007 ◽  
pp. 59-81
Author(s):  
Laurie E. Locascio ◽  
Wyatt N. Vreeland ◽  
Andreas Jahn ◽  
Michael Gaitan
Keyword(s):  
Cellular Systems

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

Achievable Capacity of Closed/Open-Access Cognitive Radio Systems Coexisting with a Macro Cellular Systems

2012 ◽  
Vol E95-B (4) ◽  
pp. 1190-1197
Author(s):  
Hiromasa FUJII ◽  
Hiroki HARADA ◽  
Shunji MIURA ◽  
Hidetoshi KAYAMA
Keyword(s):  
Cognitive Radio ◽  
Open Access ◽  
Cellular Systems ◽  
Cognitive Radio Systems ◽  
Radio Systems ◽  
Macro Cellular

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

A Review on 3GPP 5G Security Aspects

2019 ◽  
Author(s):  
Rajavelsamy R ◽  
Debabrata Das
Keyword(s):  
Network Architecture ◽  
Base Station ◽  
Cellular Systems ◽  
Core Network ◽  
User Privacy ◽  
Wireless Backhaul ◽  
Level Of Use ◽  
Unified Authentication ◽  
3Rd Generation Partnership Project ◽  
5G Security

5G promises to support new level of use cases that will deliver a better user experience. The 3rd Generation Partnership Project (3GPP) [1] defined 5G system introduced fundamental changes on top of its former cellular systems in several design areas, including security. Unlike in the legacy systems, the 5G architecture design considers Home control enhancements for roaming customer, tight collaboration with the 3rd Party Application servers, Unified Authentication framework to accommodate various category of devices and services, enhanced user privacy, and secured the new service based core network architecture. Further, 3GPP is investigating the enhancements to the 5G security aspects to support longer security key lengths, False Base station detection and wireless backhaul in the Phase-2 of 5G standardization [2]. This paper provides the key enhancements specified by the 3GPP for 5G system, particularly the differences to the 4G system and the rationale behind the decisions.

Performance Analysis of Two-Hop-Relay WCDMA Cellular Systems

2011 ◽  
Vol 30 (11) ◽  
pp. 2552-2555
Author(s):  
Wei-feng Lu ◽  
Meng Wu
Keyword(s):  
Performance Analysis ◽  
Cellular Systems

Statistical Analysis of the Uplink Inter-cell Interference for Cellular Systems

2014 ◽  
Vol 35 (8) ◽  
pp. 1971-1976 ◽  
Author(s):  
Yuan-ping Zhu ◽  
Jing Xu ◽  
Yang Yang ◽  
Jiang Wang
Keyword(s):  
Statistical Analysis ◽  
Cellular Systems ◽  
Inter Cell Interference

Superoxide Anion Radical: Generation and Detection in Cellular and Non-Cellular Systems

2015 ◽  
Vol 22 (37) ◽  
pp. 4234-4256 ◽  
Author(s):  
Renan Chiste ◽  
Marisa Freitas ◽  
Adriana Mercadante ◽  
Eduarda Fernandes
Keyword(s):  
Superoxide Anion ◽  
Anion Radical ◽  
Cellular Systems ◽  
Superoxide Anion Radical ◽  
Radical Generation
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