Integrating Functional Genomics With Salinity and Water Deficit Stress Responses in Wine Grape - Vitis Vinifera

2007 ◽  
pp. 643-668 ◽  
Author(s):  
Jérôme Grimplet ◽  
Laurent G. Deluc ◽  
Grant R. Cramer ◽  
John C. Cushman
Keyword(s):  
Vitis Vinifera ◽  
Functional Genomics ◽  
Water Deficit ◽  
Stress Responses ◽  
Water Deficit Stress ◽  
Wine Grape

scholarly journals Myxospermous seed-mucilage quantity correlates with environmental gradients indicative of water-deficit stress: Plantago species as a model

2019 ◽  
Vol 446 (1-2) ◽  
pp. 343-356 ◽  
Author(s):  
António Teixeira ◽  
Pietro Iannetta ◽  
Kirsty Binnie ◽  
Tracy A. Valentine ◽  
Peter Toorop
Keyword(s):  
Seed Germination ◽  
Water Deficit ◽  
Stress Responses ◽  
Environmental Gradients ◽  
Seedling Survival ◽  
Water Deficit Stress ◽  
Arid Environments ◽  
Seedling Mortality ◽  
The Relationship ◽  
Seed Mucilage

Abstract Aims Myxospermous seed mucilage is multifunctional and is often found in seeds (or achenes) of species occupying arid environments where the trait may influence seed-dispersal and -germination of seeds. The seed mucilage may also enhance soil-water retention, −hydraulic conductivity and -stability. However, the relationship between seed mucilage quantity, seed germination and seedling traits across environmental gradients which determine water-deficit stress has not yet been ascertained. Methods Therefore, we characterised and tested the relationship between seed mucilage quantity, water-deficit stress responses of seeds and seedlings of 36 accessions of four different Plantago species (P. albicans L., P. coronopus L., P. lagopus L. and P. anceolata L.). These were gathered from six regions across Europe, which presented environmental gradients (of rainfall and temperature), and varying soil qualities. Results Seed mucilage was significantly greater in seeds of accessions experiencing: highest summer temperatures; lowest summer precipitation; soils of the same warm dry regions which had greater capacity to retain water within narrow pore spaces. Under water-deficit stress, seeds with most mucilage exhibited a lower base water potential for germination, suffered least seedling mortality and exhibited the most successful seedling development. Conclusions The findings indicate that seed mucilage quantity appeared as an ‘adaptive’ trait and there is a relationship between seed-mucilage quantity, seed germination plus seedling survival and development under environmental conditions of highest water-deficit stress.

scholarly journals Physiology and transcriptomics of water-deficit stress responses in wheat cultivars TAM 111 and TAM 112

2014 ◽  
Vol 171 (14) ◽  
pp. 1289-1298 ◽  
Author(s):  
Srirama Krishna Reddy ◽  
Shuyu Liu ◽  
Jackie C. Rudd ◽  
Qingwu Xue ◽  
Paxton Payton ◽  
...  
Keyword(s):  
Water Deficit ◽  
Stress Responses ◽  
Water Deficit Stress ◽  
Wheat Cultivars

Effects of exogenous nitric oxide on growth, proline accumulation and antioxidant capacity in Cakile maritima seedlings subjected to water deficit stress

2016 ◽  
Vol 43 (10) ◽  
pp. 939 ◽  
Author(s):  
Asma Jday ◽  
Kilani Ben Rejeb ◽  
Ines Slama ◽  
Kaouthar Saadallah ◽  
Marianne Bordenave ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Water Deficit ◽  
Stress Responses ◽  
Lipid Membrane ◽  
Proline Accumulation ◽  
Shoot Biomass ◽  
Water Deficit Stress ◽  
Leaf Water Content ◽  
Protein Levels ◽  
Cakile Maritima

Nitric oxide (NO) – an endogenous signalling molecule in plants and animals – mediates responses to biotic and abiotic stresses. In the present study, we examined the role of exogenous application of NO in mediating stress responses in Cakile maritima Scop. seedlings under water deficit stress using sodium nitroprusside (SNP) as NO donor and as a pre-treatment before the application of stress. Water deficit stress was applied by withholding water for 14 days. Growth, leaf water content (LWC), osmotic potential (ψs), chlorophyll, malondialdehyde (MDA), electrolyte leakage (EL), proline and Δ1-pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) protein levels were determined. Enzyme activities involved in antioxidant activities (superoxide dismutase (SOD) and catalase (CAT)) were measured upon withholding water. The results showed that shoot biomass production was significantly decreased in plants subjected to water deficit stress alone. However, in water deficit stressed plants pre-treated with SNP, growth activity was improved and proline accumulation was significantly increased. Proline accumulation was concomitant with the stimulation of its biosynthesis as shown by the accumulation of P5CS proteins. Nevertheless, no significant change in ProDH protein levels was observed. Besides plants showed lower water deficit-induced lipid membrane degradation and oxidative stress after the pretreatment with 100 µM SNP. This behaviour was related to the increased activity of SOD and CAT. Thus, we concluded that NO increased C. maritima drought tolerance and mitigated damage associated with water deficit stress by the regulation of proline metabolism and the reduction of oxidative damage.

scholarly journals Quantitative proteome profile of water deficit stress responses in eastern cottonwood (Populus deltoides) leaves

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0190019 ◽  
Author(s):  
Paul E. Abraham ◽  
Benjamin J. Garcia ◽  
Lee E. Gunter ◽  
Sara S. Jawdy ◽  
Nancy Engle ◽  
...  
Keyword(s):  
Water Deficit ◽  
Stress Responses ◽  
Populus Deltoides ◽  
Water Deficit Stress ◽  
Eastern Cottonwood ◽  
Proteome Profile

Genotypic water-deficit stress responses in durum wheat: association between physiological traits, microRNA regulatory modules and yield components

2017 ◽  
Vol 44 (5) ◽  
pp. 538 ◽  
Author(s):  
Haipei Liu ◽  
Amanda J. Able ◽  
Jason A. Able
Keyword(s):  
Durum Wheat ◽  
Stress Tolerance ◽  
Water Deficit ◽  
Stress Responses ◽  
Yield Components ◽  
Physiological Responses ◽  
Physiological Traits ◽  
Water Deficit Stress ◽  
Genotypic Differences ◽  
Time Points

In Mediterranean environments, water-deficit stress that occurs before anthesis significantly limits durum wheat (Triticum turgidum L. ssp. durum) production. Stress tolerant and stress sensitive durum varieties exhibit genotypic differences in their response to pre-anthesis water-deficit stress as reflected by yield performance, but our knowledge of the mechanisms underlying tolerance is limited. We have previously identified stress responsive durum microRNAs (miRNAs) that could contribute to water-deficit stress tolerance by mediating post-transcriptional silencing of genes that lead to stress adaptation (e.g. miR160 and its targets ARF8 (auxin response factor 8) and ARF18). However, the temporal regulation pattern of miR160-ARFs after induction of pre-anthesis water-deficit stress in sensitive and tolerant varieties remains unknown. Here, the physiological responses of four durum genotypes are described by chlorophyll content, leaf relative water content, and stomatal conductance at seven time-points during water-deficit stress from booting to anthesis. qPCR examination of miR160, ARF8 and ARF18 at these time-points revealed a complex stress responsive regulatory pattern, in the flag leaf and the head, subject to genotype. Harvest components and morphological traits measured at maturity confirmed the stress tolerance level of these four varieties for agronomic performance, and their potential association with the physiological responses. In general, the distinct regulatory pattern of miR160-ARFs among stress tolerant and sensitive durum varieties suggests that miRNA-mediated molecular pathways may contribute to the genotypic differences in the physiological traits, ultimately affecting yield components (e.g. the maintenance of harvest index and grain number).

scholarly journals Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula

2021 ◽  
Vol 22 (6) ◽  
pp. 2813
Author(s):  
Verónica Castañeda ◽  
Esther M. González
Keyword(s):  
Drought Stress ◽  
Water Deficit ◽  
Stress Responses ◽  
Carbon Metabolism ◽  
Crop Productivity ◽  
Invertase Activity ◽  
Water Deficit Stress ◽  
Plant Responses ◽  
Whole Plant ◽  
Plant Level

Water-deficit stresses such as drought and salinity are the most important factors limiting crop productivity. Hence, understanding the plant responses to these stresses is key for the improvement of their tolerance and yield. In this study M. truncatula plants were subjected to 250 mM NaCl as well as reduced irrigation (No-W) and 250 g/L polyethylene glycol (PEG)-6000 to induce salinity and drought stress, respectively, provoking a drop to −1.7 MPa in leaf water potential. The whole plant physiology and metabolism was explored by characterizing the stress responses at root, phloem sap and leaf organ level. PEG treatment led to some typical responses of plants to drought stress, but in addition to PEG uptake, an important impairment of nutrient uptake and a different regulation of carbon metabolism could be observed compared to No-W plants. No-W plants showed an important redistribution of antioxidants and assimilates to the root tissue, with a distinctive increase in root proline degradation and alkaline invertase activity. On the contrary, salinity provoked an increase in leaf starch and isocitrate dehydrogenase activity, suggesting key roles in the plant response to this stress. Overall, results suggest higher protection of salt-stressed shoots and non-irrigated roots through different mechanisms, including the regulation of proline and carbon metabolism, while discarding PEG as safe mimicker of drought. This raises the need to understand the effect at the whole plant level of the different strategies employed to apply water-deficit stress.

scholarly journals Water-deficit responsive microRNAs in the primary root growth zone of maize

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Candace M. Seeve ◽  
Ramanjulu Sunkar ◽  
Yun Zheng ◽  
Li Liu ◽  
Zhijie Liu ◽  
...  
Keyword(s):  
Root Growth ◽  
Water Deficit ◽  
Stress Responses ◽  
Primary Root ◽  
Growth Zone ◽  
Water Deficit Stress ◽  
Mild Stress ◽  
Nutrient Deficiencies ◽  
Mirna Regulation ◽  
Primary Root Growth

Abstract Background MicroRNA-mediated gene regulatory networks play a significant role in plant growth and development and environmental stress responses. Results We identified 79 microRNAs (miRNAs) and multiple miRNA variants (isomiRs) belonging to 26 miRNA families in the primary root growth zone of maize seedlings grown at one of three water potentials: well-watered (− 0.02 MPa), mild water deficit stress (− 0.3 MPa), and severe water deficit stress (− 1.6 MPa). The abundances of 3 miRNAs (mild stress) and 34 miRNAs representing 17 families (severe stress) were significantly different in water-deficit stressed relative to well-watered controls (FDR < 0.05 and validated by stem loop RT-qPCR). Degradome sequencing revealed 213 miRNA-regulated transcripts and trancriptome profiling revealed that the abundance of 77 (miRNA-regulated) were regulated by water-defecit stress. miR399e,i,j-3p was strongly regulated by water-defcit stress implicating the possibility of nutrient deficiency during stress. Conclusions We have identified a number of maize miRNAs that respond to specific water deficits applied to the primary root growth zone. We have also identified transcripts that are targets for miRNA regulation in the root growth zone under water-deficit stress. The miR399e,i,j-3p that is known to regulate phosphate uptake in response to nutrient deficiencies responds to water-deficit stress, however, at the seedling stage the seed provides adequate nutrients for root growth thus miR399e,i,j-3p may play a separate role in water-deficit responses. A water-deficit regulated maize transcript, similar to known miR399 target mimics, was identified and we hypothesized that it is another regulatory player, moderating the role of miR399e,i,j-3p, in primary root growth zone water deficit responses.

scholarly journals Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato

2019 ◽  
Vol 20 (3) ◽  
pp. 761 ◽  
Author(s):  
Nihao Jiang ◽  
Xueying Fan ◽  
Weipeng Lin ◽  
Guoping Wang ◽  
Kunzheng Cai
Keyword(s):  
Water Deficit ◽  
Stress Responses ◽  
Bacterial Wilt ◽  
Resistance Mechanism ◽  
Transcriptional Response ◽  
Differentially Expressed ◽  
Water Deficit Stress ◽  
Post Inoculation ◽  
Molecular Pattern

Bacterial wilt is a devastating disease of tomato caused by soilborne pathogenic bacterium Ralstonia solanacearum. Previous studies found that silicon (Si) can increase tomato resistance against R. solanacearum, but the exact molecular mechanism remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes of root transcriptome profiles between Si-treated (+Si) and untreated (−Si) tomato plants at 1, 3, and 7 days post-inoculation with R. solanacearum. The contents of salicylic acid (SA), ethylene (ET), and jasmonic acid (JA) and the activity of defense-related enzymes in roots of tomato in different treatments were also determined. The burst of ET production in roots was delayed, and SA and JA contents were altered in Si treatment. The transcriptional response to R. solanacearum infection of the +Si plants was quicker than that of the untreated plants. The expression levels of differentially-expressed genes involved in pathogen-associated molecular pattern-triggered immunity (PTI), oxidation resistance, and water-deficit stress tolerance were upregulated in the Si-treated plants. Multiple hormone-related genes were differentially expressed in the Si-treated plants. Si-mediated resistance involves mechanisms other than SA- and JA/ET-mediated stress responses. We propose that Si-mediated tomato resistance to R. solanacearum is associated with activated PTI-related responses and enhanced disease resistance and tolerance via several signaling pathways. Such pathways are mediated by multiple hormones (e.g., SA, JA, ET, and auxin), leading to diminished adverse effects (e.g., senescence, water-deficit, salinity and oxidative stress) normally caused by R. solanacearum infection. This finding will provide an important basis to further characterize the role of Si in enhancing plant resistance against biotic stress.

scholarly journals The Role of Ethylene in Water-deficit Stress Responses in Betula papyrifera Marsh

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1392-1395 ◽  
Author(s):  
Mengmeng Gu ◽  
James A. Robbins ◽  
Curt R. Rom
Keyword(s):  
Water Deficit ◽  
Stress Responses ◽  
Water Status ◽  
Leaf Abscission ◽  
Water Deficit Stress ◽  
Minor Role ◽  
Betula Papyrifera ◽  
Physiological Level ◽  
Paper Birch ◽  
Ethylene Action

One-year-old paper birch (Betula papyrifera Marsh.) seedlings were exposed to water deficit, ethylene, or inhibitors of ethylene action under greenhouse conditions to investigate ethylene's role in water-deficit stress-induced leaf abscission. Exposing well-watered and water-stressed paper birch to 20 ppm ethylene resulted in more than 50% leaf abscission after 96 h regardless of plant water status. However, application of a physiological level (1 ppm) of ethylene did not cause leaf abscission in either well-watered or water-stressed paper birch. Inhibitors of ethylene action (1ppm 1-methylcyclopropene or 0.1 mm silver thiosulfate) did not affect predawn water potential, gas exchange, or chlorophyll fluorescence. A significant increase in ethylene production was not detected in water-stressed paper birch before the onset of significant leaf abscission. Based on these observations, ethylene would appear to play a minor role in water-deficit stress-induced leaf abscission in paper birch.

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