Crystallization Methods of Membrane Proteins: Practical Aspects of Crystallizing Plant Light-Harvesting Complexes

2008 ◽  
pp. 77-96 ◽  
Author(s):  
Zhenfeng Liu ◽  
Wenrui Chang
Keyword(s):  
Membrane Proteins ◽  
Light Harvesting ◽  
Light Harvesting Complexes

scholarly journals Noise-Assisted Discord-Like Correlations in Light-Harvesting Photosynthetic Complexes

2021 ◽  
Vol 3 (2) ◽  
pp. 262-271
Author(s):  
Pablo Reséndiz-Vázquez ◽  
Ricardo Román-Ancheyta ◽  
Roberto León-Montiel
Keyword(s):  
Potential Role ◽  
Energy Transport ◽  
Light Harvesting ◽  
Quantum Correlations ◽  
Transport Processes ◽  
Quantum Evolution ◽  
Light Harvesting Complexes ◽  
Transport Efficiency ◽  
Photovoltaic Materials ◽  
Photosynthetic Complexes

Transport phenomena in photosynthetic systems have attracted a great deal of attention due to their potential role in devising novel photovoltaic materials. In particular, energy transport in light-harvesting complexes is considered quite efficient due to the balance between coherent quantum evolution and decoherence, a phenomenon coined Environment-Assisted Quantum Transport (ENAQT). Although this effect has been extensively studied, its behavior is typically described in terms of the decoherence’s strength, namely weak, moderate or strong. Here, we study the ENAQT in terms of quantum correlations that go beyond entanglement. Using a subsystem of the Fenna–Matthews–Olson complex, we find that discord-like correlations maximize when the subsystem’s transport efficiency increases, while the entanglement between sites vanishes. Our results suggest that quantum discord is a manifestation of the ENAQT and highlight the importance of beyond-entanglement correlations in photosynthetic energy transport processes.

scholarly journals Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane

Life ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Radek Kaňa ◽  
Gábor Steinbach ◽  
Roman Sobotka ◽  
György Vámosi ◽  
Josef Komenda
Keyword(s):  
Membrane Proteins ◽  
Single Molecule ◽  
Protein Interactions ◽  
Thylakoid Membrane ◽  
Protein Complexes ◽  
Correlation Spectroscopy ◽  
Membrane Microdomains ◽  
Fast Diffusion ◽  
Light Harvesting Complexes ◽  
Term Survival

Biological membranes were originally described as a fluid mosaic with uniform distribution of proteins and lipids. Later, heterogeneous membrane areas were found in many membrane systems including cyanobacterial thylakoids. In fact, cyanobacterial pigment–protein complexes (photosystems, phycobilisomes) form a heterogeneous mosaic of thylakoid membrane microdomains (MDs) restricting protein mobility. The trafficking of membrane proteins is one of the key factors for long-term survival under stress conditions, for instance during exposure to photoinhibitory light conditions. However, the mobility of unbound ‘free’ proteins in thylakoid membrane is poorly characterized. In this work, we assessed the maximal diffusional ability of a small, unbound thylakoid membrane protein by semi-single molecule FCS (fluorescence correlation spectroscopy) method in the cyanobacterium Synechocystis sp. PCC6803. We utilized a GFP-tagged variant of the cytochrome b6f subunit PetC1 (PetC1-GFP), which was not assembled in the b6f complex due to the presence of the tag. Subsequent FCS measurements have identified a very fast diffusion of the PetC1-GFP protein in the thylakoid membrane (D = 0.14 − 2.95 µm2s−1). This means that the mobility of PetC1-GFP was comparable with that of free lipids and was 50–500 times higher in comparison to the mobility of proteins (e.g., IsiA, LHCII—light-harvesting complexes of PSII) naturally associated with larger thylakoid membrane complexes like photosystems. Our results thus demonstrate the ability of free thylakoid-membrane proteins to move very fast, revealing the crucial role of protein–protein interactions in the mobility restrictions for large thylakoid protein complexes.

scholarly journals Uncovering the interactions driving carotenoid binding in light-harvesting complexes

2021 ◽  
Author(s):  
Vincenzo Mascoli ◽  
Nicoletta Liguori ◽  
Lorenzo Cupellini ◽  
Eduard Elias ◽  
Benedetta Mennucci ◽  
...  
Keyword(s):  
Protein Stability ◽  
Light Harvesting ◽  
Light Harvesting Complexes

Carotenoids are essential constituents of plant light-harvesting complexes (LHCs), being involved in protein stability, light harvesting, and photoprotection. Unlike chlorophylls, whose binding to LHCs is known to require coordination of...

scholarly journals The molecular mechanisms of light adaption in light-harvesting complexes of purple bacteria revealed by a multiscale modeling

2019 ◽  
Vol 10 (42) ◽  
pp. 9650-9662 ◽  
Author(s):  
Felipe Cardoso Ramos ◽  
Michele Nottoli ◽  
Lorenzo Cupellini ◽  
Benedetta Mennucci
Keyword(s):  
Multiscale Modeling ◽  
Molecular Mechanisms ◽  
Light Harvesting ◽  
Purple Bacteria ◽  
Spectral Tuning ◽  
Light Harvesting Complexes

The spectral tuning of LH2 antenna complexes arises from H-bonding, acetyl torsion, and inter-chromophore couplings.

Quantum Coherent Energy Transfer over Varying Pathways in Single Light-Harvesting Complexes

Science ◽  
2013 ◽  
Vol 340 (6139) ◽  
pp. 1448-1451 ◽  
Author(s):  
R. Hildner ◽  
D. Brinks ◽  
J. B. Nieder ◽  
R. J. Cogdell ◽  
N. F. van Hulst
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
Light Harvesting Complexes

Fluorescence microscopy on single light-harvesting complexes

1999 ◽  
Author(s):  
Carsten Tietz ◽  
Alexander Draebenstedt ◽  
Joerg Schuster ◽  
Joerg Wrachtrup
Keyword(s):  
Fluorescence Microscopy ◽  
Light Harvesting ◽  
Light Harvesting Complexes

Open quantum system parameters for light harvesting complexes from molecular dynamics

2015 ◽  
Vol 17 (38) ◽  
pp. 25629-25641 ◽  
Author(s):  
Xiaoqing Wang ◽  
Gerhard Ritschel ◽  
Sebastian Wüster ◽  
Alexander Eisfeld
Keyword(s):  
Molecular Dynamics ◽  
Quantum System ◽  
Open Quantum System ◽  
Light Harvesting ◽  
Parameter Extraction ◽  
Extraction Methods ◽  
Light Harvesting Complexes ◽  
System Parameters ◽  
Open Quantum ◽  
The Difference

We elucidate the difference between various parameter extraction methods and demonstrate sensitivity to molecular dynamics equilibration.

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