Differentiation Between Malignant and Benign Pleural Effusions: Methylation Specific Polymerase Chain Reaction Analysis

2008 ◽  
pp. 337-347
Author(s):  
Susana Benlloch ◽  
Josè Marcelo Galbis-Caravajal ◽  
Bartomeu Massutí
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Pleural Effusions ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

122-PA10 Polymerase chain reaction in diagnostic pleural effusions

1995 ◽  
Vol 76 ◽  
pp. 14
Author(s):  
G. Yilmaz ◽  
B. Utku ◽  
A.R. Küçükusta ◽  
N. Çine ◽  
T. Özçelik
Keyword(s):  
Polymerase Chain Reaction ◽  
Pleural Effusions ◽  
Chain Reaction ◽  
Polymerase Chain
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