Combining Data from “Related” Experiments

2010 ◽  
pp. 173-192
Author(s):  
Francisco J. Samaniego
Keyword(s):  
Combining Data

scholarly journals Looking at the Nudibranch Family Myrrhinidae (Gastropoda, Heterobranchia) from a Mitochondrial ‘2D Folding Structure’ Point of View

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 583
Author(s):  
Giulia Furfaro ◽  
Paolo Mariottini
Keyword(s):  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Integrative Taxonomy ◽  
Point Of View ◽  
Past Study ◽  
Mitochondrial Coi ◽  
The Past ◽  
Combining Data ◽  
Folding Structure ◽  
Comparison Of The Results

Integrative taxonomy is an evolving field of multidisciplinary studies often utilised to elucidate phylogenetic reconstructions that were poorly understood in the past. The systematics of many taxa have been resolved by combining data from different research approaches, i.e., molecular, ecological, behavioural, morphological and chemical. Regarding molecular analysis, there is currently a search for new genetic markers that could be diagnostic at different taxonomic levels and that can be added to the canonical ones. In marine Heterobranchia, the most widely used mitochondrial markers, COI and 16S, are usually analysed by comparing the primary sequence. The 16S rRNA molecule can be folded into a 2D secondary structure that has been poorly exploited in the past study of heterobranchs, despite 2D molecular analyses being sources of possible diagnostic characters. Comparison of the results from the phylogenetic analyses of a concatenated (the nuclear H3 and the mitochondrial COI and 16S markers) dataset (including 30 species belonging to eight accepted genera) and from the 2D folding structure analyses of the 16S rRNA from the type species of the genera investigated demonstrated the diagnostic power of this RNA molecule to reveal the systematics of four genera belonging to the family Myrrhinidae (Gastropoda, Heterobranchia). The “molecular morphological” approach to the 16S rRNA revealed to be a powerful tool to delimit at both species and genus taxonomic levels and to be a useful way of recovering information that is usually lost in phylogenetic analyses. While the validity of the genera Godiva, Hermissenda and Phyllodesmium are confirmed, a new genus is necessary and introduced for Dondice banyulensis, Nemesis gen. nov. and the monospecific genus Nanuca is here synonymised with Dondice, with Nanuca sebastiani transferred into Dondice as Dondice sebastiani comb. nov.

scholarly journals Estimating epidemiologic dynamics from cross-sectional viral load distributions

Science ◽  
2021 ◽  
pp. eabh0635
Author(s):  
James A. Hay ◽  
Lee Kennedy-Shaffer ◽  
Sanjat Kanjilal ◽  
Niall J. Lennon ◽  
Stacey B. Gabriel ◽  
...  
Keyword(s):  
Population Distribution ◽  
Quantitative Polymerase Chain Reaction ◽  
Cross Sectional ◽  
Outbreak Management ◽  
Viral Loads ◽  
Time Estimates ◽  
Load Distributions ◽  
Random Samples ◽  
Combining Data ◽  
Polymerase Chain

Estimating an epidemic’s trajectory is crucial for developing public health responses to infectious diseases, but case data used for such estimation are confounded by variable testing practices. We show that the population distribution of viral loads observed under random or symptom-based surveillance, in the form of cycle threshold (Ct) values obtained from reverse-transcription quantitative polymerase chain reaction testing, changes during an epidemic. Thus, Ct values from even limited numbers of random samples can provide improved estimates of an epidemic’s trajectory. Combining data from multiple such samples improves the precision and robustness of such estimation. We apply our methods to Ct values from surveillance conducted during the SARS-CoV-2 pandemic in a variety of settings and offer alternative approaches for real-time estimates of epidemic trajectories for outbreak management and response.

scholarly journals Relationships between test positivity rate, total laboratory confirmed cases of malaria, and malaria incidence in high burden settings of Uganda: an ecological analysis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Jaffer Okiring ◽  
Adrienne Epstein ◽  
Jane F. Namuganga ◽  
Victor Kamya ◽  
Asadu Sserwanga ◽  
...  
Keyword(s):  
Regression Models ◽  
Malaria Incidence ◽  
Temporal Changes ◽  
Linear Regression Models ◽  
High Burden ◽  
Exponential Regression ◽  
Malaria Morbidity ◽  
Laboratory Test Results ◽  
Combining Data ◽  
Catchment Areas

Abstract Background Malaria surveillance is critical for monitoring changes in malaria morbidity over time. National Malaria Control Programmes often rely on surrogate measures of malaria incidence, including the test positivity rate (TPR) and total laboratory confirmed cases of malaria (TCM), to monitor trends in malaria morbidity. However, there are limited data on the accuracy of TPR and TCM for predicting temporal changes in malaria incidence, especially in high burden settings. Methods This study leveraged data from 5 malaria reference centres (MRCs) located in high burden settings over a 15-month period from November 2018 through January 2020 as part of an enhanced health facility-based surveillance system established in Uganda. Individual level data were collected from all outpatients including demographics, laboratory test results, and village of residence. Estimates of malaria incidence were derived from catchment areas around the MRCs. Temporal relationships between monthly aggregate measures of TPR and TCM relative to estimates of malaria incidence were examined using linear and exponential regression models. Results A total of 149,739 outpatient visits to the 5 MRCs were recorded. Overall, malaria was suspected in 73.4% of visits, 99.1% of patients with suspected malaria received a diagnostic test, and 69.7% of those tested for malaria were positive. Temporal correlations between monthly measures of TPR and malaria incidence using linear and exponential regression models were relatively poor, with small changes in TPR frequently associated with large changes in malaria incidence. Linear regression models of temporal changes in TCM provided the most parsimonious and accurate predictor of changes in malaria incidence, with adjusted R2 values ranging from 0.81 to 0.98 across the 5 MRCs. However, the slope of the regression lines indicating the change in malaria incidence per unit change in TCM varied from 0.57 to 2.13 across the 5 MRCs, and when combining data across all 5 sites, the R2 value reduced to 0.38. Conclusions In high malaria burden areas of Uganda, site-specific temporal changes in TCM had a strong linear relationship with malaria incidence and were a more useful metric than TPR. However, caution should be taken when comparing changes in TCM across sites.

Social Media and Emotional Well-being: Pursuit of Happiness or Pleasure

2021 ◽  
pp. 1326365X2110037
Author(s):  
D. Guna Graciyal ◽  
Deepa Viswam
Keyword(s):  
Social Media ◽  
Social Networking ◽  
Virtual World ◽  
Mixed Methods Research ◽  
Well Being ◽  
Pursuit Of Happiness ◽  
Social Dysfunction ◽  
Combining Data ◽  
Social Media Platforms ◽  
Depth Interviews

Virtual engagement of lives has been made possible with the advent of social media. Almost 80% of the day are spent virtually on Facebook, Instagram, Twitter, YouTube, Snapchat, etc. Usage of social media to connect to and communicate with the ones we care about is always healthy, termed as social networking. Social dysfunction occurs when the constant communication leads to the point where our real or offline life gets replaced by virtual or online life. There is a slight boundary between social networking and social dysfunction. When social networking is advantageous, social dysfunction affects emotional well-being. When emotional well-being is affected, many users experience a compulsion to dissociate from the real world as they find virtual world, full of fantasy and enjoyment. When the Internet was created, perhaps no one was aware of its potential. More than the convenience for sharing of information it has brought the world so close to crumbling the geographical boundaries. The more people-to-people communication is, the more is the strengthening of relationships, bonds grow stronger with ‘more’ social media platforms. Being on ‘more’ social media platforms has become a benchmark for living amidst the younger generation. Either as an activity of happiness or as an activity of pleasure, users tend to use social media at varying levels. This paper aims to conceptualize the the intricacies of social media in young lives and to discern whether their association is happiness or pleasure activity. The research method of this paper has a mixed-methods research design combining data from structured survey with information outputs from in-depth interviews.

scholarly journals Retail Policies and Urban Change in Naples City Center: Challenges to Resilience and Sustainability from a Mediterranean City

2021 ◽  
Vol 13 (14) ◽  
pp. 7620
Author(s):  
Rosario Sommella ◽  
Libera D’Alessandro
Keyword(s):  
Field Research ◽  
Urban Resilience ◽  
City Center ◽  
Political Discourses ◽  
Sustainability Research ◽  
Combining Data ◽  
Consumption Practices ◽  
Adaptive Resilience ◽  
Policy And Planning ◽  
The Impact

Political discourses, public discussions, and studies in different fields have increasingly focused on the vulnerabilities affecting cities and on the possible responses to them, which are often traced back to urban resilience and sustainability. Research and debates in the field of retailing and consumption geographies are no exception. To carry out a critical analysis on the retail policies associated with the urban commercial change of the Naples city center, the case study is placed in the context of the literature review focusing on three concepts: spatial vulnerability, adaptive resilience, and territorialized sustainability. The analysis is conducted combining data, policy, and planning documents with long-term field research. The changing relationship between consumption practices, retail dynamics, and policies highlights a sort of hybridization of commercial and consumption central cityscapes, which is produced by the coexistence between retail-led phenomena of regeneration and forms of local resistance. The results of the research highlight, from a Mediterranean perspective, new general insights on the impact of selective forms of vulnerability and on the adaptive resilience strategies adopted, but most of all on the indispensable rethinking of the urban retail governance for the enhancement of urban livability, social cohesion, and locally sustainable lifestyles, activities, and places.

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