Developmental Expression of Proto-Oncogene c-erb-A Related Genes Encoding Nuclear Receptors in Xenopus

1990 ◽  
pp. 447-451
Author(s):  
Jamshed R. Tata ◽  
Betty S. Baker
Keyword(s):  
Nuclear Receptors ◽  
Developmental Expression ◽  
Genes Encoding

scholarly journals Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes.

1986 ◽  
Vol 6 (7) ◽  
pp. 2409-2419 ◽  
Author(s):  
A Villasante ◽  
D Wang ◽  
P Dobner ◽  
P Dolph ◽  
S A Lewis ◽  
...  
Keyword(s):  
Duplication Event ◽  
Developmental Expression ◽  
Untranslated Regions ◽  
Coding Region ◽  
Specific Expression ◽  
Translational Initiation ◽  
Alpha Tubulin ◽  
Tubulin Isotypes ◽  
Tubulin Isotype ◽  
Genes Encoding

Five mouse alpha-tubulin isotypes are described, each distinguished by the presence of unique amino acid substitutions within the coding region. Most, though not all of these isotype-specific amino acids, are clustered at the carboxy terminus. One of the alpha-tubulin isotypes described is expressed exclusively in testis and is encoded by two closely related genes (M alpha 3 and M alpha 7) which have homologous 3' untranslated regions but which differ at multiple third codon positions and in their 5' untranslated regions. We show that a subfamily of alpha-tubulin genes encoding the same testis-specific isotype also exists in humans. Thus, we conclude that the duplication event leading to a pair of genes encoding a testis-specific alpha-tubulin isotype predated the mammalian radiation, and both members of the duplicated sequence have been maintained since species divergence. A second alpha-tubulin gene, M alpha 6, is expressed ubiquitously at a low level, whereas a third gene, M alpha 4, is unique in that it does not encode a carboxy-terminal tyrosine residue. This gene yields two transcripts: a 1.8-kilobase (kb) mRNA that is abundant in muscle and a 2.4-kb mRNA that is abundant in testis. Whereas the 1.8-kb mRNA encodes a distinct alpha-tubulin isotype, the 2.4-kb mRNA is defective in that the methionine residue required for translational initiation is missing. Patterns of developmental expression of the various alpha-tubulin isotypes are presented. Our data support the view that individual tubulin isotypes are capable of conferring functional specificity on different kinds of microtubules.

Identification of a putative expansin gene expressed in the subventral glands of the cereal cyst nematode Heterodera avenae

Nematology ◽  
2012 ◽  
Vol 14 (5) ◽  
pp. 571-577 ◽  
Author(s):  
Haibo Long ◽  
Deliang Peng ◽  
Wenkun Huang ◽  
Yanke Liu ◽  
Huan Peng
Keyword(s):  
Cyst Nematode ◽  
Developmental Expression ◽  
Heterodera Avenae ◽  
Parasitic Nematodes ◽  
Secretory Proteins ◽  
Expansin Gene ◽  
Genes Encoding ◽  
Juvenile Stages ◽  
Stage Process ◽  
Parasitism Genes

Parasitism genes encoding secretory proteins expressed in the pharyngeal glands of plant-parasitic nematodes play important roles in the parasitic process. A new expansin gene (Ha-expb1) expressed in the subventral glands of the sedentary cyst nematode, Heterodera avenae, was cloned. Southern blot analysis suggested that Ha-expb1 is a member of a multigene family. The deduced protein Ha-EXPB1 consists of a signal peptide, a CBM II and an expansin domain, and was significantly similar to expansins and expansin-like proteins from the potato cyst nematode, Globodera rostochiensis, and the pine wood nematodes, Bursaphelenchus spp. In situ hybridisation showed that Ha-expb1 transcript specifically accumulated in the two subventral gland cells of the second-stage juveniles. Developmental expression confirmed that its transcript abundances were high in the motile juvenile stages and low in the sedentary stage of the nematode, implying a role in the early parasitic-stage process, most likely in aiding migration within the plant.

Abstract 191: Transcriptional Regulation of Renin by Nuclear Receptors Co-regulated With Renin

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Ko-Ting Lu ◽  
Eric T Weatherford ◽  
Pimonrat Ketsawatsomkron ◽  
Justin L Grobe ◽  
Curt D Sigmund
Keyword(s):  
Gene Expression ◽  
Nuclear Receptors ◽  
Estrogen Receptor Alpha ◽  
Hormone Receptors ◽  
Cell Types ◽  
Negative Control ◽  
Sirna Duplex ◽  
Expression Of Genes ◽  
Renin Gene ◽  
Genes Encoding

Expression of the renin gene is required to maintain normal morphological and physiological identity of renal juxtaglomerular (JG) cells, yet the mechanisms regulating renin gene transcription remain elusive. We re-examined data from Brunskill et. al (JASN 22:2213, 2011), investigating genome-wide gene expression in JG and other renal cell types. Based on our previous data implicating nuclear receptors (RAR, RXR, VDR, PPARG, Nr2f2 and Nr2f6) in the regulation of mouse and human renin gene expression, we focused our analysis on the expression of genes encoding the 48 nuclear hormone receptors and their co-regulation with renin. Several nuclear receptors have an expression pattern emulating that of renin, that is, they were similarly enriched in JG cells but not in other cell types. These include Esr1, Nr1h4, Ppara, VDR, Nr1i2, Ppard, Hnf4g, Nr1h3, Thrb, Hnf4a, Esrrg, Nr4a3, Nr3c2, and Ar. We tested the hypothesis that a nuclear receptor that is co-regulated with renin may participate in renin gene regulation. To accomplish this, endogenous renin expression was evaluated in renin-expressing As4.1 cells after siRNA-mediated knock down of selected nuclear receptors. Each experiment included a negative control siRNA duplex (NC) that does not target any known genes. By way of example, siRNA-mediated inhibition of estrogen receptor alpha (Esr1) by 70-80% resulted in a 2-fold decrease in renin mRNA (fold change ± SEM: siEsr1: 0.4±0.2, p<0.001 vs NC). Similar results were obtained with a different siRNA targeting Esr1. Interestingly, loss of Esr1 also caused up-regulation of vitamin D receptor (VDR, 2.8±0.7 fold, p<0.001 vs NC) and Nr2f6 (2.0±0.2 fold, p<0.05 vs NC), both of which are known to be negative regulators of renin. Similarly, both renin (0.1±0.02, p<0.001 vs untreated) and Esr1 (0.3±0.1, p<0.05 vs untreated) mRNA were reduced in the kidney from mice treated with deoxycorticosterone acetate (50mg) and receiving 0.15 M NaCl in drinking water for 21 days (DOCA-salt). These data suggest Esr1 may regulate renin expression. Studies are in progress to assess if Esr1 stimulates renin expression on its own or acts by affecting the level of other nuclear receptors; and to determine if other co-regulated nuclear receptors also regulate expression of the renin gene.

Characterization and developmental expression of genes encoding the early carotenoid biosynthetic enzymes in Citrus paradisi Macf.

2011 ◽  
Vol 39 (2) ◽  
pp. 895-902 ◽  
Author(s):  
Marcio G. C. Costa ◽  
Cristina D. Moreira ◽  
John R. Melton ◽  
Wagner C. Otoni ◽  
Gloria A. Moore
Keyword(s):  
Developmental Expression ◽  
Biosynthetic Enzymes ◽  
Citrus Paradisi ◽  
Expression Of Genes ◽  
Genes Encoding

scholarly journals Two PR-1 Genes from Tomato Are Differentially Regulated and Reveal a Novel Mode of Expression for a Pathogenesis-Related Gene During the Hypersensitive Response and Development

1997 ◽  
Vol 10 (5) ◽  
pp. 624-634 ◽  
Author(s):  
Pablo Tornero ◽  
José Gadea ◽  
Vicente Conejero ◽  
Pablo Vera
Keyword(s):  
Expression Pattern ◽  
Hypersensitive Response ◽  
Constitutive Expression ◽  
Developmental Expression ◽  
Gus Gene ◽  
Cortical Cells ◽  
Pathogenesis Related ◽  
Genes Encoding ◽  
Refractory State ◽  
Pathogenesis Related Gene

Pathogenesis-related (PR) proteins form a heterogeneous family of plant proteins that are likely to be involved in defense and are inducible by pathogen attacks. One group of PRs, represented by the subfamily PR-1, are low-molecular-weight proteins of unknown biochemical function. Here we describe the cloning and characterization of two closely related genes encoding a basic and an acidic PR-1 protein (PR1b1 and PR1a2) from tomato (Lycopersicon esculentum). We present a comparative study of the mode of transcriptional regulation of these two genes in transgenic tobacco plants using a series of promoter-GUS fusions. Unexpectedly, the chimeric PR1a2/GUS gene is not induced by pathogenic signals but instead shows constitutive expression with a reproducible developmental expression pattern. It is expressed in shoot meristems, trichomes, and cortical cells as well as in vascular and nearby tissues of the mature stem. This constitutive expression pattern may represent preemption of plant defenses against potential pathogens. Conversely, the chimeric PR1b1/GUS gene does not show any constitutive expression in the plant, but it is transcriptionally activated following pathogen attack. Upon infection by tobacco mosaic virus, the PR1b1 gene is strongly activated locally in tissues undergoing the hypersensitive response but not systemically in uninoculated tissues. Furthermore, its expression is induced by both salicylic acid and ethylene precursors, two signals that coexist and apparently mediate the activation of local defenses during the hypersensitive response. We speculate that the different mode of expression of the two genes presented here, together with that reported previously for the induction of other PR-1 genes in systemic, uninoculated tissues, may all be complementary and necessary for the plant to acquire an efficient refractory state to resist pathogen attacks.

Dexamethasone downregulates expression of several genes encoding orphan nuclear receptors that are important to steroidogenesis in stallion testes

2019 ◽  
Vol 33 (6) ◽  
Author(s):  
Raul Valdez ◽  
Clay A. Cavinder ◽  
Dickson D. Varner ◽  
Thomas H. Welsh ◽  
Martha M. Vogelsang ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Orphan Nuclear Receptors ◽  
Genes Encoding

Docetaxel pharmacogenetics: The influence of RXRα and HNF4α genetic variations on docetaxel disposition in Asian nasopharyngeal carcinoma patients.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 2598-2598
Author(s):  
Sin Chi Chew ◽  
Onkar Singh ◽  
Xiangai Chen ◽  
Wan-Teck Lim ◽  
Eng-Huat Tan ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
High Frequency ◽  
Direct Sequencing ◽  
Nasopharyngeal Cancer ◽  
Genetic Variations ◽  
Individual Variability ◽  
Orphan Nuclear Receptors ◽  
Linkage Pattern ◽  
Metabolism Enzymes ◽  
Genes Encoding

2598 Background: The transactivations of the metabolism enzymes (CYP3A4/5) and efflux transporters (ABCB1/ABCC2) involved in docetaxel disposition are regulated by the orphan nuclear receptors such as PXR, CAR, RXRα and HNF4α. This study aimed to explore the associations between the genetic variations present in the genes encoding the orphan nuclear receptors, RXRα and HNF4α, on docetaxel disposition. Methods: The DNAs from healthy Chinese, Malay and Indian subjects (n=56 each) were screened for RXRα and HNF4α SNPs in exons and intronic/exonic boundaries by direct sequencing. The high frequency SNPs (>1%) were profiled in a cohort of local nasopharyngeal cancer patients (n=54). Genotypic-phenotypic correlations were conducted using Mann-Whitney U-test and Kruskal-Wallis test. Results: Eighty-eight and sixty-nine SNPs were identified from the healthy screening of RXRα and HNF4α, respectively, across 3 populations. A total of 30 and 35 high frequency SNPs in RXRα and HNF4α, respectively, were profiled in the patients. Six RXRα SNPs [IVS2+33G>A (rs2234753), IVS7+70A>G (rs1536475),*846G>A (rs4240711), *+4458G>A (rs3132291), *+4768C>A (rs4842196) and *+4988A>G (rs4842198)] and 6 HNF4α SNPs [-728A>C (rs1800963), IVS2-278A>G (rs55934816), IVS6+141A>G (rs6103731), IVS7-88T>C (rs2273618), IVS9–145T>C (rs3746574) and IVS9-67C>G (rs3746575)] were significantly associated with higher clearance and lower AUC0-∞ and/or Cmax of docetaxel (P<0.05). Conversely, HNF4α SNP [IVS9+354G>T (rs3818247)] was associated with lower clearance and higher AUC0-∞ and Cmax of docetaxel (p<0.05). A high linkage pattern was observed among the abovementioned RXRα SNPs except for IVS2+33G>A (rs2234753) (D'≥0.74). Similarly, HNF4α SNPs were found to be highly linked, except for -728A>C (rs1800963) (D'≥0.62). Conclusions: The results highlight the contributions of RXRα and HNF4α pharmacogenetics in influencing the inter-individual variability in docetaxel disposition in Asian nasopharyngeal patients.

W15-P-005 Regulation of genes encoding intestinal cholesterol transporters by nuclear receptors in human CACO-2/TC7 enterocytes

2005 ◽  
Vol 6 (1) ◽  
pp. 97-98
Author(s):  
S. Lestavel ◽  
C. Duval ◽  
V. Touches ◽  
A. Tailleux ◽  
V. Carriere ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Genes Encoding ◽  
Cholesterol Transporters
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