Modulation of Cyclooxygenase-2 Expression by Apc In Ht-29 Human Colorectal Carcinoma Cells

Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusive. This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. These data suggest new directions of research regarding aspartame’s role in colorectal cancer.

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Effect of PPAR Activators on Cytokine-Stimulated Cyclooxygenase-2 Expression in Human Colorectal Carcinoma Cells

Experimental Cell Research ◽

10.1006/excr.2001.5233 ◽

2001 ◽

Vol 267 (1) ◽

pp. 73-80 ◽

Cited By ~ 28

Author(s):

Hiroshi Ikawa ◽

Hideto Kameda ◽

Hideki Kamitani ◽

Seung Joon Baek ◽

Jennifer B. Nixon ◽

...

Keyword(s):

Colorectal Carcinoma ◽

Cyclooxygenase 2 ◽

Carcinoma Cells ◽

Human Colorectal Carcinoma

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Aqueous Extract ofSolanum nigrumLeaves Induces Autophagy and Enhances Cytotoxicity of Cisplatin, Doxorubicin, Docetaxel, and 5-Fluorouracil in Human Colorectal Carcinoma Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/514719 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 13

Author(s):

Chen-Jei Tai ◽

Chien-Kai Wang ◽

Cheng-Jeng Tai ◽

Yi-Feng Lin ◽

Chi-Shian Lin ◽

...

Keyword(s):

Colorectal Cancer ◽

Colorectal Carcinoma ◽

Aqueous Extract ◽

Tumor Suppression ◽

Drug Effect ◽

Carcinoma Cells ◽

Common Cancer ◽

Human Colorectal Carcinoma ◽

And Control ◽

Ht 29

Colorectal cancer is a common cancer worldwide, and chemotherapy is a mainstream approach for advanced and recurrent cases. Development of effective complementary drugs could help improve tumor suppression efficiency and control adverse effects from chemotherapy. The aqueous extract ofSolanum nigrumleaves (AE-SN) is an essential component in many traditional Chinese medicine formulas for treating cancer, but there is a lack of evidence verifying its tumor suppression efficacy in colorectal cancer. The purpose of this study is to evaluate the tumor suppression efficacy of AE-SN using DLD-1 and HT-29 human colorectal carcinoma cells and examine the combined drug effect when combined with the chemotherapeutic drugs cisplatin, doxorubicin, docetaxel, and 5-fluorouracil. The results indicated that AE-SN induced autophagy via microtubule-associated protein 1 light chain 3 A/B II accumulation but not caspase-3-dependent apoptosis in both cell lines. The IC50s after 48 hours of treatment were 0.541 and 0.948 mg/ml AE-SN in DLD-1 and HT-29, respectively. AE-SN also demonstrated a combined drug effect with all tested drugs by enhancing cytotoxicity in tumor cells. Our results suggest that AE-SN has potential in the development of complementary chemotherapy for colorectal cancer.

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