Regulation of Biosynthesis of Carotenoids in Phycomyces Blakesleeanus

1992 ◽  
pp. 367-375
Author(s):  
H. Yokoyama ◽  
E. P. Hayman
Keyword(s):  
Phycomyces Blakesleeanus ◽  
Regulation Of Biosynthesis

scholarly journals On the phylogeny of Phycomyces blakesleeanus. Nucleotide sequence of 5 S ribosomal RNA.

1982 ◽  
Vol 257 (15) ◽  
pp. 9114-9118
Author(s):  
J Andersen ◽  
W Andresini ◽  
N Delihas
Keyword(s):  
Nucleotide Sequence ◽  
Ribosomal Rna ◽  
Phycomyces Blakesleeanus

scholarly journals Intersexual Partial Diploids of Phycomyces

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 635-641
Author(s):  
Bina J Mehta ◽  
Enrique Cerdá-Olmedo
Keyword(s):  
Dry Mass ◽  
Sexual Cycle ◽  
Genetic Constitution ◽  
Phycomyces Blakesleeanus ◽  
Carotene Content ◽  
Sexual Interaction ◽  
Sexual Stimulation ◽  
Nuclear Composition ◽  
Opposite Sex ◽  
Β Carotene

Abstract Sexual interaction between strains of opposite sex in many fungi of the order Mucorales modifies hyphal morphology and increases the carotene content. The progeny of crosses of Phycomyces blakesleeanus usually include a small proportion of anomalous segregants that show these signs of sexual stimulation without a partner. We have analyzed the genetic constitution of such segregants from crosses that involved a carF mutation for overaccumulation of β-carotene and other markers. The new strains were diploids or partial diploids heterozygous for the sex markers. Diploidy was unknown in this fungus and in the Zygomycetes. Random chromosome losses during the vegetative growth of the diploid led to heterokaryosis in the coenocytic mycelia and eventually to sectors of various tints and mating behavior. The changes in the nuclear composition of the mycelia could be followed by selecting for individual nuclei. The results impose a reinterpretation of the sexual cycle of Phycomyces. Some of the intersexual strains that carried the carF mutation contained 25 mg β-carotene per gram of dry mass and were sufficiently stable for practical use in carotene production.

scholarly journals Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joseph K. E. Ortega ◽  
Revathi P. Mohan ◽  
Cindy M. Munoz ◽  
Shankar Lalitha Sridhar ◽  
Franck J. Vernerey
Keyword(s):  
Avoidance Response ◽  
Experimental Results ◽  
Sensory Transduction ◽  
Growth Responses ◽  
Phycomyces Blakesleeanus ◽  
Sensory Stimuli ◽  
Phototropic Response ◽  
Biophysical Models ◽  
Helical Growth ◽  
Made In

AbstractThe sporangiophores of Phycomyces blakesleeanus have been used as a model system to study sensory transduction, helical growth, and to establish global biophysical equations for expansive growth of walled cells. More recently, local statistical biophysical models of the cell wall are being constructed to better understand the molecular underpinnings of helical growth and its behavior during the many growth responses of the sporangiophores to sensory stimuli. Previous experimental and theoretical findings guide the development of these local models. Future development requires an investigation of explicit and implicit assumptions made in the prior research. Here, experiments are conducted to test three assumptions made in prior research, that (a) elongation rate, (b) rotation rate, and (c) helical growth steepness, R, of the sporangiophore remain constant during the phototropic response (bending toward unilateral light) and the avoidance response (bending away from solid barriers). The experimental results reveal that all three assumptions are incorrect for the phototropic response and probably incorrect for the avoidance response but the results are less conclusive. Generally, the experimental results indicate that the elongation and rotation rates increase during these responses, as does R, indicating that the helical growth steepness become flatter. The implications of these findings on prior research, the “fibril reorientation and slippage” hypothesis, global biophysical equations, and local statistical biophysical models are discussed.

Tangent algorithm for photogravitropic balance in plants and Phycomyces blakesleeanus: roles for EHB1 and NPH3 of Arabidopsis thaliana

2021 ◽  
pp. 153396
Author(s):  
Michaela Dümmer ◽  
Sladjana Z. Spasić ◽  
Martin Feil ◽  
Christian Michalski ◽  
Christoph Forreiter ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phycomyces Blakesleeanus

scholarly journals Spatial and temporal regulation of biosynthesis of the plant immune signal salicylic acid

2015 ◽  
Vol 112 (30) ◽  
pp. 9166-9173 ◽  
Author(s):  
Xiao-yu Zheng ◽  
Mian Zhou ◽  
Heejin Yoo ◽  
Jose L. Pruneda-Paz ◽  
Natalie Weaver Spivey ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Signal Transduction Pathway ◽  
Temporal Regulation ◽  
Calmodulin Binding ◽  
Regulation Of Biosynthesis ◽  
Pathogen Entry ◽  
Transcription Activators ◽  
Transcriptional Induction

The plant hormone salicylic acid (SA) is essential for local defense and systemic acquired resistance (SAR). When plants, such as Arabidopsis, are challenged by different pathogens, an increase in SA biosynthesis generally occurs through transcriptional induction of the key synthetic enzyme isochorismate synthase 1 (ICS1). However, the regulatory mechanism for this induction is poorly understood. Using a yeast one-hybrid screen, we identified two transcription factors (TFs), NTM1-LIKE 9 (NTL9) and CCA1 HIKING EXPEDITION (CHE), as activators of ICS1 during specific immune responses. NTL9 is essential for inducing ICS1 and two other SA synthesis-related genes, PHYTOALEXIN-DEFICIENT 4 (PAD4) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), in guard cells that form stomata. Stomata can quickly close upon challenge to block pathogen entry. This stomatal immunity requires ICS1 and the SA signaling pathway. In the ntl9 mutant, this response is defective and can be rescued by exogenous application of SA, indicating that NTL9-mediated SA synthesis is essential for stomatal immunity. CHE, the second identified TF, is a central circadian clock oscillator and is required not only for the daily oscillation in SA levels but also for the pathogen-induced SA synthesis in systemic tissues during SAR. CHE may also regulate ICS1 through the known transcription activators CALMODULIN BINDING PROTEIN 60g (CBP60g) and SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) because induction of these TF genes is compromised in the che-2 mutant. Our study shows that SA biosynthesis is regulated by multiple TFs in a spatial and temporal manner and therefore fills a gap in the signal transduction pathway between pathogen recognition and SA production.

Excess carotenoids disturb prospective cell-to-cell recognition system in mating responses of Phycomyces blakesleeanus

Mycoscience ◽  
1996 ◽  
Vol 37 (4) ◽  
pp. 427-435 ◽  
Author(s):  
Tamotsu Ootaki ◽  
Yutaka Yamazaki ◽  
Toshiro Noshita ◽  
Shunya Takahashi
Keyword(s):  
Recognition System ◽  
Cell Recognition ◽  
Phycomyces Blakesleeanus

Respiratory system of Fusidium coccineum and regulation of biosynthesis of fusidic acid and sporogenesis

1981 ◽  
Vol 12 (4) ◽  
pp. 220-225 ◽  
Author(s):  
S. M. Navashin ◽  
Yu. E. Bartoshevich ◽  
G. N. Telesnina ◽  
R. A. Zvjagilskaya ◽  
S. V. Dmitriyeva ◽  
...  
Keyword(s):  
Respiratory System ◽  
Fusidic Acid ◽  
Regulation Of Biosynthesis

GENETIC RECOMBINATION IN SEXUAL CROSSES OF PHYCOMYCES

Genetics ◽  
1975 ◽  
Vol 80 (3) ◽  
pp. 445-462
Author(s):  
A P Eslava ◽  
M I Alvarez ◽  
Patricia V Burke ◽  
M Delbrück
Keyword(s):  
Mating Type ◽  
Genetic Recombination ◽  
Recombination Frequency ◽  
Intragenic Recombination ◽  
Phycomyces Blakesleeanus ◽  
Sexual Crosses ◽  
Color Marker ◽  
Three Factor

ABSTRACT Sexual crosses between strains of Phycomyces blakesleeanus, involving three auxotrophic and one color marker and yielding a high proportion of zygospore germination, are described. Samples of 20-40 germ spores from 311 individual fertile germ sporangia originating from five two-factor and three three-factor crosses were characterized. The results show: (1) absence of any contribution of apogamic nuclei to the progeny, (2) confirmation of Burgeff's conjecture that the germ spores of any germ sporangium in most cases derive from one meiosis. In a cross involving two allelic markers the analysis of 175 pooled germ sporangia suggests an intragenic recombination frequency of 0.6%. All other factor combinations tested are unlinked. The bulk of the germ spores are homokaryotic. However, a small portion (4%) are heterokaryotic with respect to mating type.

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