Pregnancy-Associated Plasma Protein-A (PAPP-A) is a Specific Inhibitor of the Third Component of Human Complement

1984 ◽  
pp. 323-333
Author(s):  
Paul Bischof ◽  
Antoine Geinoz
Keyword(s):  
Plasma Protein ◽  
Protein A ◽  
Specific Inhibitor ◽  
Human Complement ◽  
The Third

Pregnancy-associated plasma protein a (PAPP-A) specifically inhibits the third component of human complement (C3)

Placenta ◽  
1984 ◽  
Vol 5 (1) ◽  
pp. 1-7 ◽  
Author(s):  
P. Bischof ◽  
A. Geinoz ◽  
W.L. Herrmann ◽  
P.C. Sizonenko
Keyword(s):  
Plasma Protein ◽  
Protein A ◽  
Complement C3 ◽  
Human Complement ◽  
The Third

STUDIES ON PREGNANCY-ASSOCIATED PLASMA PROTEIN A IN THE THIRD TRIMESTER OF PREGNANCY

1979 ◽  
Vol 86 (11) ◽  
pp. 882-887 ◽  
Author(s):  
R. Smith ◽  
P. Bischof ◽  
G. Hughes ◽  
A. Klopper
Keyword(s):  
Plasma Protein ◽  
Protein A ◽  
Third Trimester ◽  
The Third

Application of Protein A-Rosette Assay for Screening of Monoclonal Antibodies to Human Complement Regulatory Proteins

1990 ◽  
Vol 7 (2) ◽  
pp. 78-89 ◽  
Author(s):  
Tsukasa Seya ◽  
Tomoko Hara ◽  
Akiko Uenaka ◽  
Eiichi Nakayama ◽  
Hitoshi Akedo
Keyword(s):  
Monoclonal Antibodies ◽  
Protein A ◽  
Regulatory Proteins ◽  
Human Complement ◽  
Complement Regulatory Proteins

Low maternal serum levels of pregnancy associated plasma protein A (PAPP-A) in the first trimester in association with abnormal fetal karyotype

1993 ◽  
Vol 100 (4) ◽  
pp. 324-326 ◽  
Author(s):  
B. Brambati ◽  
M. C. M. Macintosh ◽  
B. Teisner ◽  
S. Maguiness ◽  
K. Shrimanker ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Protein A ◽  
Serum Levels ◽  
First Trimester ◽  
Maternal Serum ◽  
Fetal Karyotype

scholarly journals Quantification of Proteolytically Active Pregnancy-Associated Plasma Protein-A with an Assay Based on Quenched Fluorescence

2007 ◽  
Vol 53 (5) ◽  
pp. 947-954 ◽  
Author(s):  
Claus Gyrup ◽  
Michael Christiansen ◽  
Claus Oxvig
Keyword(s):  
Proteolytic Activity ◽  
Plasma Protein ◽  
Basic Protein ◽  
Biological Function ◽  
Limit Of Detection ◽  
Protein A ◽  
Maternal Serum ◽  
Serum Samples ◽  
A Subunit ◽  
Eosinophil Major Basic Protein

Abstract Background: Maternal serum concentrations of pregnancy-associated plasma protein-A (PAPP-A, pappalysin-1, EC 3.4.24.79) are used to predict the occurrence of Down syndrome. In pregnancy, PAPP-A primarily circulates as a covalent 2:2 complex with the proform of eosinophil major basic protein (proMBP), which inhibits the proteolytic activity of PAPP-A. At term, however, ∼1% of PAPP-A exists as an active, uncomplexed dimer with proteolytic activity directed specifically toward insulin-like growth factor binding protein (IGFBP)-4 and IGFBP-5. No assays have been developed that allow quantification of PAPP-A proteolytic activity. Methods: We developed a sensitive and specific immunocapture assay for PAPP-A activity based on intramolecular quenched fluorescence. We used a 26-residue synthetic peptide derived from IGFBP-4 in which specific positions on each side of the PAPP-A cleavage site were substituted with 3-nitrotyrosine and o-aminobenzoic acid. Results: The assay detected the activity of recombinant PAPP-A as well as PAPP-A in serum samples from pregnant women. The limit of detection (mean signal of blank plus 3 SD) of the active PAPP-A subunit was 13 pmol/L, and the intra- and interassay CVs were <10% and <15%, respectively. Interestingly, the fraction of active PAPP-A decreased gradually from week 7 to week 19 of pregnancy. Conclusions: This method allows the measurement of PAPP-A enzymatic activity and because of its specificity it is relevant to the study of the biological function of PAPP-A. The method may also be useful in the diagnosis of pregnancy disorders.

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