General Properties of Isolated Protoplasts and Uptake of Foreign Genetic Material

1977 ◽  
pp. 321-327 ◽  
Author(s):  
E. C. Cocking
Keyword(s):  
Genetic Material ◽  
Isolated Protoplasts ◽  
General Properties

scholarly journals Transduction mechanisms of bacteriophage ε15I. General properties of the system

1971 ◽  
Vol 18 (1) ◽  
pp. 9-19 ◽  
Author(s):  
R. W. Hedges
Keyword(s):  
High Frequency ◽  
Genetic Material ◽  
Recipient Strain ◽  
The Other ◽  
Donor Strain ◽  
General Properties ◽  
Show Evidence ◽  
Transduction Mechanisms ◽  
Defective Prophage

SUMMARYBacteriophageεγis capable of transduction both by replacement of a genetic segment of the recipient by the homologous genetic material from the donor strain and by the formation of defective transducing particles capable of lysogenizing the recipient strain ofS. anatum.The isolation of strains carrying such prophages, which have incorporated the lactose or arabinose operons, is reported. Lysogenic strains, carrying both normal and defective transducing prophage, form high-frequency transducing lysates. Other strains, carrying only defective prophage, show evidence that the association of prophage genes and transduced materials is stable since the loss of one frequently entails loss of the other.

Studies on Isolated Protoplasts and Vacuoles: I. GENERAL PROPERTIES

1966 ◽  
Vol 17 (1) ◽  
pp. 57-67 ◽  
Author(s):  
D. W. GREGORY ◽  
E. C. COCKING
Keyword(s):  
Isolated Protoplasts ◽  
General Properties

The zoonotic potential of bat-borne coronaviruses

2020 ◽  
Vol 4 (4) ◽  
pp. 365-381
Author(s):  
Ny Anjara Fifi Ravelomanantsoa ◽  
Sarah Guth ◽  
Angelo Andrianiaina ◽  
Santino Andry ◽  
Anecia Gentles ◽  
...  
Keyword(s):  
Genetic Material ◽  
Field Research ◽  
Sympatric Species ◽  
Animal Origin ◽  
Mammalian Host ◽  
Past Century ◽  
The Past ◽  
Novel Host ◽  
Horseshoe Bat ◽  
Human Infections

Seven zoonoses — human infections of animal origin — have emerged from the Coronaviridae family in the past century, including three viruses responsible for significant human mortality (SARS-CoV, MERS-CoV, and SARS-CoV-2) in the past twenty years alone. These three viruses, in addition to two older CoV zoonoses (HCoV-229E and HCoV-NL63) are believed to be originally derived from wild bat reservoir species. We review the molecular biology of the bat-derived Alpha- and Betacoronavirus genera, highlighting features that contribute to their potential for cross-species emergence, including the use of well-conserved mammalian host cell machinery for cell entry and a unique capacity for adaptation to novel host environments after host switching. The adaptive capacity of coronaviruses largely results from their large genomes, which reduce the risk of deleterious mutational errors and facilitate range-expanding recombination events by offering heightened redundancy in essential genetic material. Large CoV genomes are made possible by the unique proofreading capacity encoded for their RNA-dependent polymerase. We find that bat-borne SARS-related coronaviruses in the subgenus Sarbecovirus, the source clade for SARS-CoV and SARS-CoV-2, present a particularly poignant pandemic threat, due to the extraordinary viral genetic diversity represented among several sympatric species of their horseshoe bat hosts. To date, Sarbecovirus surveillance has been almost entirely restricted to China. More vigorous field research efforts tracking the circulation of Sarbecoviruses specifically and Betacoronaviruses more generally is needed across a broader global range if we are to avoid future repeats of the COVID-19 pandemic.

Indole-3-acetic acid uptake in isolated protoplasts of the moss Funaria hygrometrica

1990 ◽  
Vol 80 (4) ◽  
pp. 584-592 ◽  
Author(s):  
Ulrike Geier ◽  
Olaf Werner ◽  
Martin Bopp
Keyword(s):  
Acetic Acid ◽  
Acid Uptake ◽  
Funaria Hygrometrica ◽  
Isolated Protoplasts ◽  
Indole 3 Acetic Acid

Evaluation of radiation-induced genotoxicity on hu-man melanoma cells (SK-MEL-37) by flow cytometry

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Leticia Bonfim ◽  
Luma Ramirez de Carvalho ◽  
Daniel Perez Vieira
Keyword(s):  
Genetic Material ◽  
Micronucleus Assay ◽  
Melanoma Cells ◽  
Dead Cell ◽  
Genotoxic Damage ◽  
Sytox Green ◽  
60Co Source ◽  
Radiation Induced ◽  
Death And Loss ◽  
Cell Fractions

Micronucleus assay is a test used to evaluate genotoxic damage in cells, which can be caused by various factors, like ionizing radiation. Interactions between radiation energies and DNA can cause breakage, leading to use chromosomal mutations or loss of genetic material, important events that could be induced in solid tumors to mitigate its expansion within human body.  Melanoma has been described as a tumor with increased radio resistance.  This work evaluated micronuclei percentages (%MN) in human melanoma cells (SK-MEL-37), irradiated by gamma radiation, with doses between 0 and 16Gy.  Cell suspensions were irradiated in PBS by a 60Co source in doses between 0 and 16Gy, and incubated by 48h.  Then cell membranes were lysed in the presence of SYTOX Green and EMA dyes, preserving nuclear membranes.  Using this method, EMA-stained nuclei could be discriminated as those derived from dead cells, and SYTOX nuclei and micronuclei could be quantified.  Micronuclei percentages were found to be proportional to dose, (R2 = 0.997).  Only the highest dose (16Gy) could induce statistically significant increase of MN (p<0.0001), although cultures irradiated by 4, 8 and 16Gy showed significant increase of dead cell fractions.  Calculation of the nuclei-to-beads ratio showed that 8 and 16Gy could reduce melanoma cell proliferation.  Results showed that although cell death and loss of proliferative capacity could be observed on cultures irradiated at lower doses, genotoxic damage could be induced only on a higher dose. Resistance to radiation-induced genotoxicity could explain a relatively high radio resistance of melanoma tumors.

Cryobanking of Amphibian Genetic Recourses in Russia: Past and Future

2019 ◽  
Vol 26 (6) ◽  
pp. 319-324
Author(s):  
Victor K. Uteshev ◽  
Edith N. Gakhova ◽  
Ludmila I. Kramarova ◽  
Natalia V. Shishova ◽  
Svetlana A. Kaurova ◽  
...  
Keyword(s):  
Genetic Material ◽  
Reproductive Technologies ◽  
Cell Biophysics ◽  
Amphibian Species ◽  
Modern Biology ◽  
Rare And Endangered Species ◽  
Long Term Storage ◽  
Current State ◽  
Rare And Endangered ◽  
Academy Of Sciences

In modern biology, a search for efficient and safe ways of long-term storage of animal genomes is vital for the survival of rare and endangered species. To date, the only reliable method of prolonging the preservation of genome is deep freezing of somatic and reproductive cells, including spermatozoa. Here we overview the current state of reproductive technologies and present the cryopresevation strategies of genetic material of selected amphibian species. These strategies were developed at the Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia. Cryopreservation could help managing the threatened populations of amphibian species through genome storage and mediate the production of healthy animals from the stored material.

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