Involvement of mitogen-activated protein kinases and reactive oxygen species in the inotropic action of ouabain on cardiac myocytes. A potential role for mitochondrial KATP channels

2003 ◽  
pp. 181-187 ◽  
Author(s):  
Jiang Tian ◽  
Jiang Liu ◽  
Keith D. Garlid ◽  
Joseph I. Shapiro ◽  
Zijian Xie
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Kinases ◽  
Cardiac Myocytes ◽  
Potential Role ◽  
Katp Channels ◽  
Oxygen Species ◽  
Inotropic Action ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein ◽  
Mitochondrial Katp Channels

scholarly journals Reduction of Intracellular-Reactive Oxygen Species and Diminished Mitogen-Activated Protein Kinases (MAPKs) Activation are Associated with Oral Squamous Cell Carcinoma Cell Aggressiveness

2017 ◽  
Vol 15 (2) ◽  
pp. 131-141
Author(s):  
Tanyarath UTAIPAN ◽  
Apsorn SATTAYAKHOM ◽  
Issara PRACHONGSAI ◽  
Nurdina CHARONG ◽  
Warangkana CHUNGLOK
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Protein Kinases ◽  
Cell Lines ◽  
Oxygen Species ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

Oral squamous cell carcinoma (OSCC) is a serious health problem in many countries. Several drugs have been used to treat head and neck and oral cavity cancers. However, the success rate has not been impressive because of the heterogeneity of cancerous cells, resulting in differential responsiveness to chemotherapy. Two distinct phenotypes of OSCC cells, the CLS-354/WT and CLS-354/DXcells, have been used as in vitro cell models for this study. CLS-354/DXcells were more aggressive than CLS-354/WTcells, supported by the observation that CLS-354/DXcells can undergo epithelial-mesenchymal transition (EMT), grow anchorage-independently, and increase invasiveness. We investigated the preliminary redox status of these 2 cell lines, including levels of reactive oxygen species (ROS) and cellular antioxidants, using flow cytometry analysis and ABTS+ free radical scavenging assay, respectively. A 7-fold decrease in ROS level was detected in CLS-354/DXcells, comparing with CLS-354/WTcells, while antioxidant capacity was not different from that of CLS-354/WT cells. Hydrogen peroxide, a ROS modulating agent, could induce ROS levels, and caused cell death in CLS-354/WT greater than that of CLS-354/DX cells. Of note, hydrogen peroxide-induced cytotoxicity could be rescued by N-acetyl cysteine, confirming ROS-mediated cytotoxicity in both cell lines. ROS-sensitive mitogen-activated protein kinases (MAPKs) were observed using immunoblot assay. The expressions of p-JNK1/2 and p-p38 MAPK in CLS-354/DX cells were absent, while these expressions were abundantly detected in CLS-354/WTcells. This suggests that lower ROS levels, with the concomitant reduction of JNK and p38 MAPK activation in CLS-354/DX cells, are associated with cancer cell aggressiveness. These findings provide significant evidence of the resistance to ROS-modulating agents in aggressive OSCC cells.

Ellipticine induces apoptosis in human endometrial cancer cells: The potential involvement of reactive oxygen species and mitogen-activated protein kinases

Toxicology ◽  
2011 ◽  
Vol 289 (2-3) ◽  
pp. 91-102 ◽  
Author(s):  
Ji Young Kim ◽  
Seung Gee Lee ◽  
Jin-Yong Chung ◽  
Yoon-Jae Kim ◽  
Ji-Eun Park ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endometrial Cancer ◽  
Cancer Cells ◽  
Protein Kinases ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

scholarly journals Effect of silver nanoparticles on mitogen-activated protein kinases activation: role of reactive oxygen species and implication in DNA damage

Mutagenesis ◽  
2014 ◽  
Vol 30 (1) ◽  
pp. 59-66 ◽  
Author(s):  
A. Rinna ◽  
Z. Magdolenova ◽  
A. Hudecova ◽  
M. Kruszewski ◽  
M. Refsnes ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Protein Kinases ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

Osthole Regulates Inflammatory Mediator Expression through Modulating NF-κB, Mitogen-Activated Protein Kinases, Protein Kinase C, and Reactive Oxygen Species

2010 ◽  
Vol 58 (19) ◽  
pp. 10445-10451 ◽  
Author(s):  
Pei-Chun Liao ◽  
Shih-Chang Chien ◽  
Chen-Lung Ho ◽  
Eugene I-Chen Wang ◽  
Shu-Ching Lee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Protein Kinases ◽  
Inflammatory Mediator ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitogen Activated Protein Kinases ◽  
Kinase C ◽  
Mitogen Activated Protein
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