The Molecular Role of the PufX Protein in Bacterial Photosynthetic Electron Transfer

Author(s):  
Francesco Francia ◽  
Paola Turina ◽  
B. Andrea Melandri ◽  
Giovanni Venturoli
2001 ◽  
Vol 29 (4) ◽  
pp. 452-455 ◽  
Author(s):  
F. J. Lown ◽  
A. T. Watson ◽  
S. Purton

We are using a molecular-genetic approach to investigate the role of nuclear genes in the biogenesis of the electron transfer complexes of mitochondria and chloroplasts. Our analysis of nuclear mutants of the green alga Chlamydomonas that are defective in respiration or photosynthesis has led to the identification of genes encoding factors required for the expression of specific organellar genes, and genes encoding structural components of the complexes.


2019 ◽  
Author(s):  
Felix Buchert ◽  
Laura Mosebach ◽  
Philipp Gäbelein ◽  
Michael Hippler

AbstractProton Gradient Regulation 5 (PGR5) is involved in the control of photosynthetic electron transfer but its mechanistic role is not yet clear. Several models have been proposed to explain phenotypes such as a diminished steady state proton motive force (pmf) and increased photodamage of photosystem I (PSI). Playing a regulatory role in cyclic electron flow (CEF) around PSI, PGR5 contributes indirectly to PSI protection by enhancing photosynthetic control, which is a pH-dependent downregulation of electron transfer at the cytochrome b6f complex (b6f). Here, we re-evaluated the role of PGR5 in the green alga Chlamydomonas reinhardtii and conclude that pgr5 possesses a dysfunctional b6f. Our data indicate that the b6f low-potential chain redox activity likely operated in two distinct modes – via the canonical Q cycle during linear electron flow and via an alternative Q cycle during CEF, attributing a ferredoxin-plastoquinone reductase activity to the b6f. The latter mode allowed efficient oxidation of the low-potential chain in the WT b6f. A switch between the two Q cycle modes was dependent of PGR5 and relied on unknown stromal electron carrier(s), which were a general requirement for b6f activity. In CEF-favouring conditions the electron transfer bottleneck in pgr5 was the b6f and insufficient flexibility in the low-potential chain redox tuning might account for the mutant pmf phenotype and the secondary consequences. Models of our findings are discussed.


Author(s):  
Lars Mohrhusen ◽  
Jessica Kräuter ◽  
Katharina Al-Shamery

The photochemical conversion of organic compounds on tailored transition metal oxide surfaces by (UV) irradiation has found wide applications ranging from the production of chemicals to the degradation of organic...


2019 ◽  
Vol 3 (9) ◽  
Author(s):  
Liping Yu ◽  
Qimin Yan ◽  
Adrienn Ruzsinszky

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