Urinary Excretion of Lithogenic Substances and Inhibitory Activity Towards Calcium-Oxalate-Monohydrate Crystal Growth in Idiopathic Urolithiasis

Urolithiasis ◽  
1989 ◽  
pp. 217-218
Author(s):  
D. Wangoo ◽  
H. Sidhu ◽  
S. Vaidyanathan ◽  
V. Rattan ◽  
S. K. Thind ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Urinary Excretion ◽  
Calcium Oxalate ◽  
Inhibitory Activity ◽  
Calcium Oxalate Monohydrate

Urinary Crystal Growth: Effect of Inhibitor Mixtures

1981 ◽  
Vol 61 (4) ◽  
pp. 487-491 ◽  
Author(s):  
P. G. Werness ◽  
Jan H. Bergert ◽  
Karen E. Lee
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Inhibitory Activity ◽  
Calcium Oxalate Monohydrate ◽  
Growth Effect ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Measured Activity ◽  
Normal Urine ◽  
The Individual

1. The crystal growth inhibitory activity of mixtures of known inhibitors and of mixtures of known inhibitors with normal urine was determined in calcium oxalate monohydrate and hydroxyapatite seeded crystal growth systems. 2. The inhibitory activity of the mixtures was compared with the measured activity of the individual components of the mixtures. All mixtures had inhibitory activity equal to the sum of the activities of their components, with the exception of RNA/urine mixtures in the calcium oxalate monohydrate system. 3. RNA/urine mixtures had inhibitory activity toward calcium oxalate monohydrate crystal growth which was less than would be predicted from the activity of the RNA and of the urine which were added. This reduced inhibitory activity was shown to be due probably to hydrolysis of RNA by the ribonuclease activity normally present in urine. 4. The results of these experiments make it possible to determine quantitatively the contribution of various naturally occurring urinary crystal growth inhibitors to the total measured inhibition observed in urine.

Surface effects in the crystal growth of calcium oxalate monohydrate

1987 ◽  
Vol 118 (2) ◽  
pp. 379-386 ◽  
Author(s):  
R.P. Singh ◽  
S.S. Gaur ◽  
D.J. White ◽  
G.H. Nancollas
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Surface Effects ◽  
Calcium Oxalate Monohydrate

Exploring the Molecular Level Interaction of Human Serum Albumin with Calcium Oxalate Monohydrate Crystals

2021 ◽  
Vol 28 ◽  
Author(s):  
Priyadarshini ◽  
Abhishek Negi ◽  
Chetna Faujdar ◽  
Lokesh Nigam ◽  
Naidu Subbarao
Keyword(s):  
Amino Acids ◽  
Crystal Growth ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Calcium Oxalate ◽  
Human Serum ◽  
Stone Formation ◽  
Calcium Oxalate Monohydrate ◽  
Calcium Oxalate Crystallization ◽  
In Silico Docking

Background: Human serum albumin (HSA) is one of the most abundant proteins in the blood plasma, urine as well as in the organic matrix of renal calculi. Macromolecules present in the urine modulate kidney stone formation either by stimulating or inhibiting crystallization process. Objective: In the present study, effect of HSA protein on the growth of calcium oxalate monohydrate crystal (COM) was investigated. Methods: Crystal growth assay was used to measure oxalate depletion in the crystal seeded solution in the presence of HSA. HSA concentrations exhibiting effect on crystal growth were selected for FTIR and XRD analysis. In silico docking was performed on seven different binding sites of HSA. Results: Albumin is playing dual role in growth of calcium oxalate crystallization. FTIR and XRD studies further revealed HSA exerted strain over crystal thus affecting its structure by interacting with amino acids of its pocket 1. Docking results indicate that out of 7 binding pocket in protein, calcium oxalate interacts with Arg-186 and Lys-190 amino acids of pocket 1. Conclusion: Our study confirms the role of HSA in calcium oxalate crystallization where acidic amino acids arginine and lysine are binding with COM crystals, revealing molecular interaction of macromolecule and crystal in urolithiasis.

The effect of warfarin on urine calcium oxalate crystal growth inhibition and urinary excretion of calcium and nephrocalcin

1993 ◽  
Vol 53 (4) ◽  
pp. 242-248 ◽  
Author(s):  
Elaine M. Worcester ◽  
James L. Sebastian ◽  
Janet G. Hiatt ◽  
Ann M. Beshensky ◽  
James A. Sadowski
Keyword(s):  
Crystal Growth ◽  
Urinary Excretion ◽  
Growth Inhibition ◽  
Calcium Oxalate ◽  
Calcium Oxalate Crystal ◽  
Urine Calcium ◽  
Crystal Growth Inhibition

Epitaxial Relationships in Urolithiasis: The Brushite—Whewellite System

1977 ◽  
Vol 52 (2) ◽  
pp. 143-148 ◽  
Author(s):  
J. L. Meyer ◽  
J. H. Bergert ◽  
L. H. Smith
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Calcium Oxalate Monohydrate ◽  
Supersaturated Solution ◽  
Scanning Electron Micrographs ◽  
Phosphate Dihydrate ◽  
Acid Environment ◽  
Time Required ◽  
Insight Into ◽  
Scanning Electron

1. Whewellite (calcium oxalate monohydrate) crystals were found to induce epitaxially the heterogeneous nucleation of brushite (calcium monohydrogen phosphate dihydrate) from its metastable supersaturated solution in approximately one-quarter of the time required for spontaneous precipitation in the absence of added nucleating agents. Scanning electron-microscope observation of the crystalline phase showed brushite crystals originating from the whewellite seed crystals. 2. Crystal growth, upon nucleation, proceeded rapidly, and the metastable solutions quickly approached saturation. 3. Brushite crystals also induced the precipitation of calcium oxalate crystals in about one-quarter of the time required for spontaneous precipitation; however, the rate of crystal growth was considerably slower. In support of the chemical data, scanning electron micrographs showed few crystals of calcium oxalate nucleated on the surface of the brushite seed. 4. The results provide some insight into the cause of stones containing calcium oxalate or calcium phosphate (or both), which form in the normally acid environment of human urine.

scholarly journals ISOLATION OF URINARY HEPARAN SULFATE AND CHONDROITIN SULFATE AND THEIR INHIBITORY ACTIVITY OF CALCIUM OXALATE CRYSTAL GROWTH

1991 ◽  
Vol 82 (3) ◽  
pp. 405-411 ◽  
Author(s):  
Hidenobu Iwata ◽  
Takashi Terado ◽  
Masahiro Kin ◽  
Shunji Nishio ◽  
Masafumi Takeuchi ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Heparan Sulfate ◽  
Chondroitin Sulfate ◽  
Inhibitory Activity ◽  
Calcium Oxalate Crystal

Isolation of calcium oxalate crystal growth inhibitor from rat kidney and urine

1984 ◽  
Vol 247 (5) ◽  
pp. F765-F772 ◽  
Author(s):  
Y. Nakagawa ◽  
V. Abram ◽  
F. L. Coe
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Column Chromatography ◽  
Dissociation Constants ◽  
Rat Kidney ◽  
Deae Cellulose ◽  
Growth Inhibitor ◽  
Calcium Oxalate Monohydrate ◽  
Heat Denaturation ◽  
Kidney Homogenate

Glycoproteins that slow the growth rate of calcium oxalate monohydrate crystals were purified from rat kidney homogenate and urine by selective heat denaturation (for rat kidney homogenate), DEAE-cellulose column chromatography, and gel permeation column chromatography. Both kidney and urine inhibitors were glycoproteins with an apparent mol wt of 1.4 X 10(4), as determined by high-performance liquid chromatography. They contained gamma-carboxyglutamic acid and a high percentage of aspartic acid and glutamic acid but had few aromatic amino acid residues. Both inhibitors contained fucose, mannose, glucose, galactose, glucosamine, galactosamine, and N-acetylneuraminic acid but no glucuronic acid. Kinetic studies suggest that purified inhibitors bind to calcium oxalate monohydrate seed crystals according to a Langmuir adsorption isotherm with similar dissociation constants of 14 X 10(-8)M for rat urine inhibitor and 8.7 X 10(-8) M for rat kidney inhibitor. The isolation of similar glycoproteins from kidney and urine suggests that urinary crystal growth inhibitor may be produced in the kidneys.

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