Histochemical Staining Using Lectin Probes

2014 ◽  
pp. 153-163 ◽  
Author(s):  
Yoshihiro Akimoto ◽  
Hayato Kawakami
Keyword(s):  
Histochemical Staining

A Fast Histochemical Staining Method to Identify Hyaline Droplets in the Rat Kidney

2003 ◽  
Vol 31 (4) ◽  
pp. 462-464 ◽  
Author(s):  
Eveline P. C. T. de Rijk ◽  
Wilma T. M. Ravesloot ◽  
Yvonne Wijnands ◽  
Eric van Esch
Keyword(s):  
Staining Method ◽  
Rat Kidney ◽  
Histochemical Staining

scholarly journals Anatomy and Histochemistry of Seed Coat Development of Wild (Pisum sativum subsp. elatius (M. Bieb.) Asch. et Graebn. and Domesticated Pea (Pisum sativum subsp. sativum L.)

2021 ◽  
Vol 22 (9) ◽  
pp. 4602
Author(s):  
Lenka Zablatzká ◽  
Jana Balarynová ◽  
Barbora Klčová ◽  
Pavel Kopecký ◽  
Petr Smýkal
Keyword(s):  
Pisum Sativum ◽  
Seed Coat ◽  
Developmental Stages ◽  
Histochemical Staining ◽  
Ruthenium Red ◽  
Detailed Knowledge ◽  
Maternal Plant ◽  
Wild Progenitor ◽  
Strong Signal ◽  
Biochemical Studies

In angiosperms, the mature seed consists of embryo, endosperm, and a maternal plant-derived seed coat (SC). The SC plays a role in seed filling, protects the embryo, mediates dormancy and germination, and facilitates the dispersal of seeds. SC properties have been modified during the domestication process, resulting in the removal of dormancy, mediated by SC impermeability. This study compares the SC anatomy and histochemistry of two wild (JI64 and JI1794) and two domesticated (cv. Cameor and JI92) pea genotypes. Histochemical staining of five developmental stages: 13, 21, 27, 30 days after anthesis (DAA), and mature dry seeds revealed clear differences between both pea types. SC thickness is established early in the development (13 DAA) and is primarily governed by macrosclereid cells. Polyanionic staining by Ruthenium Red indicated non homogeneity of the SC, with a strong signal in the hilum, the micropyle, and the upper parts of the macrosclereids. High peroxidase activity was detected in both wild and cultivated genotypes and increased over the development peaking prior to desiccation. The detailed knowledge of SC anatomy is important for any molecular or biochemical studies, including gene expression and proteomic analysis, especially when comparing different genotypes and treatments. Analysis is useful for other crop-to-wild-progenitor comparisons of economically important legume crops.

Bovine milk esterases

1971 ◽  
Vol 38 (2) ◽  
pp. 171-177 ◽  
Author(s):  
B. J. Kitchen
Keyword(s):  
Ammonium Sulphate ◽  
Bovine Milk ◽  
Skim Milk ◽  
Histochemical Staining ◽  
Major Type ◽  
Polyacrylamide Gels ◽  
Selective Inhibitors ◽  
Choline Ester ◽  
Ultra Filtration ◽  
Ammonium Sulphate Fractionation

SummaryThe type and distribution of esterases in milk has been investigated using selective inhibitors during normal assay procedures and during histochemical staining of polyacrylamide gels. Enzyme solutions were obtained from skim-milk by acid and alkali precipitation, followed by ammonium sulphate fractionation, ultra-filtration and Sephadex G-100 chromatography. The major type of esterase present was an aryl-esterase (E.C. 5.1.1.2) while a smaller amount of a choline-ester hydrolase (E.C. 3.1.1.7; 3.1.1.8) was detected. The significance of these findings is discussed.

scholarly journals Aluminon: its limited application as a reagent for the detection of aluminum species.

1985 ◽  
Vol 33 (7) ◽  
pp. 729-732 ◽  
Author(s):  
R A Clark ◽  
G L Krueger
Keyword(s):  
Tricarboxylic Acid ◽  
Histochemical Staining ◽  
Spectrophotometric Analysis ◽  
Colorimetric Determination ◽  
Pertinent Literature ◽  
Color Formation ◽  
Color Development ◽  
Inorganic Species ◽  
Limited Application

The triammonium salt of aurin tricarboxylic acid, commonly referred to as aluminon, forms a dye that has been used for the colorimetric determination of Al(III) species. We have reviewed the pertinent literature on the reaction of aluminon with respect to the metallic species that form colored aluminon complexes. The effects of experimental variables, such as time, temperature, and pH, upon the color development of the aluminon complex are also presented. Organic and inorganic species, particularly Be(II) and Fe(III), which can affect color formation, are described. The use of aluminon as a histochemical staining agent for the detection of aluminum requires verification by atomic absorption spectrophotometric analysis or other quantitative techniques.

Intraoral papillary endothelial hyperplasia: case discussion with supportive histochemistry and immunohistochemistry

2005 ◽  
Vol 29 (3) ◽  
pp. 253-257 ◽  
Author(s):  
A. Raghu ◽  
S. Tandon ◽  
N. Rao ◽  
R. Singh ◽  
V. Rekha
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cell ◽  
Histochemical Staining ◽  
Endothelial Cell Proliferation ◽  
Histological Findings ◽  
Case Discussion ◽  
Clinical Behavior ◽  
Regular Monitoring ◽  
Papillary Endothelial Hyperplasia ◽  
Unusual Lesion

An intraoral mass of eight months duration in a six year-old girl was diagnosed as papillary endothelial hyperplasia. Histologically, the tissue was characterized by the unusual endothelial cell proliferation, which is significant, as papillary endothelial hyperplasia resembles angiosarcoma and possible over-treatment thereafter. Clarification of this unusual lesion based on histological findings with supportive histochemical staining and immunohistochemistry in the light of clinico-pathological correlation is discussed. This lesion warrants better documentation of the clinical behavior with regular monitoring.

Sign in / Sign up

Export Citation Format

Share Document