scholarly journals Anatomy and Histochemistry of Seed Coat Development of Wild (Pisum sativum subsp. elatius (M. Bieb.) Asch. et Graebn. and Domesticated Pea (Pisum sativum subsp. sativum L.)

2021 ◽  
Vol 22 (9) ◽  
pp. 4602
Author(s):  
Lenka Zablatzká ◽  
Jana Balarynová ◽  
Barbora Klčová ◽  
Pavel Kopecký ◽  
Petr Smýkal
Keyword(s):  
Pisum Sativum ◽  
Seed Coat ◽  
Developmental Stages ◽  
Histochemical Staining ◽  
Ruthenium Red ◽  
Detailed Knowledge ◽  
Maternal Plant ◽  
Wild Progenitor ◽  
Strong Signal ◽  
Biochemical Studies

In angiosperms, the mature seed consists of embryo, endosperm, and a maternal plant-derived seed coat (SC). The SC plays a role in seed filling, protects the embryo, mediates dormancy and germination, and facilitates the dispersal of seeds. SC properties have been modified during the domestication process, resulting in the removal of dormancy, mediated by SC impermeability. This study compares the SC anatomy and histochemistry of two wild (JI64 and JI1794) and two domesticated (cv. Cameor and JI92) pea genotypes. Histochemical staining of five developmental stages: 13, 21, 27, 30 days after anthesis (DAA), and mature dry seeds revealed clear differences between both pea types. SC thickness is established early in the development (13 DAA) and is primarily governed by macrosclereid cells. Polyanionic staining by Ruthenium Red indicated non homogeneity of the SC, with a strong signal in the hilum, the micropyle, and the upper parts of the macrosclereids. High peroxidase activity was detected in both wild and cultivated genotypes and increased over the development peaking prior to desiccation. The detailed knowledge of SC anatomy is important for any molecular or biochemical studies, including gene expression and proteomic analysis, especially when comparing different genotypes and treatments. Analysis is useful for other crop-to-wild-progenitor comparisons of economically important legume crops.

Ruthenium Red Staining of Human Hard Keratin Fibers

1982 ◽  
Vol 40 ◽  
pp. 278-279
Author(s):  
M. C. Buhrer ◽  
R. A. Mathews
Keyword(s):  
Human Hair ◽  
Ruthenium Red ◽  
Acid Mucopolysaccharides ◽  
Biochemical Studies ◽  
Keratin Fibers ◽  
Ruthenium Red Staining ◽  
Extracellular Materials

Ruthenium red has been used as a stain to demonstrate a variety of extracellular materials, especially acid mucopolysaccharides. It also reacts with certain intracellular and extracellular lipids. Since biochemical studies in our laboratory demonstrated the presence of a variety of monosaccharides in human hair ruthenium red staining procedures were adopted in order to evaluate the presence and morphological location of acid oligosaccharides in the keratinized aspect of hair.

The Fine Structure of the Sheath of Volvox Carteri f. Weismannia

1977 ◽  
Vol 35 ◽  
pp. 346-347
Author(s):  
Regina Birchem
Keyword(s):  
Developmental Stages ◽  
Ruthenium Red ◽  
Sulfated Polysaccharides ◽  
Volvox Carteri ◽  
High Voltage Electron Microscopy ◽  
Ultrastructural Studies ◽  
Voltage Electron ◽  
Sheath Formation ◽  
And Inversion ◽  
Sheath Material

Spheroids of the green colonial alga Volvox consist of biflagellate Chlamydomonad-like cells embedded in a transparent sheath. The sheath, important as a substance through which metabolic materials, light, and the sexual inducer must pass to and from the cells, has been shown to have an ordered structure (1,2). It is composed of both protein and carbohydrate (3); studies of V. rousseletii indicate an outside layer of sulfated polysaccharides (4).Ultrastructural studies of the sheath material in developmental stages of V. carteri f. weismannia were undertaken employing variations in the standard fixation procedure, ruthenium red, diaminobenzidine, and high voltage electron microscopy. Sheath formation begins after the completion of cell division and inversion of the daughter spheroids. Golgi, rough ER, and plasma membrane are actively involved in phases of sheath synthesis (Fig. 1). Six layers of ultrastructurally differentiated sheath material have been identified.

Laboratory Studies on the Feeding Preference and Feeding Behaviour in Lygaeus equestris (L.) (Het. Lygaeidae)1

1974 ◽  
Vol 5 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Otto Kugelberg
Keyword(s):  
Seed Coat ◽  
Developmental Stages ◽  
Larval Instar ◽  
Chemical Properties ◽  
Feeding Preference ◽  
Food Plants ◽  
Short Supply ◽  
Feeding Choice ◽  
Choice Tests ◽  
Lygaeus Equestris

AbstractThe seed-feeding bug Lygaeus equestris changes and extends its food plant spectrum during its life-cycle. Whether this is the result of only the natural succession of the food plants, or also includes a change in the insect's food preference during its life, was the primary question of the present paper. Feeding-choice tests using ripe seeds from important food plants in the laboratory showed that Cynanchum vincetoxicum seeds were preferred during all stages of life. Among the lesser preferred seeds tested, a shift in preference occurred during the bug's development. It seems probable that this shift may be due as much to the physical as to the chemical properties of the seeds. It is suggested that most of the other plant species exploited for feeding by L. equestris probably serve mainly as substitute food when suitable developmental stages of C. vincetoxicum are absent or in short supply. Furthermore the paper gives some notes on duration of feeding upon seeds and effects of seed coat at initiation of feeding on Cynanchum and Helianthus seeds. During the third larval instar, the preference switches from the endosperm to the seed coat for initiation of feeding on Cynanchum seeds, and so it remains during the following stages. On Helianthus seeds, only adult L. equestris feed as willingly through the coat as directly from the endosperm.

Four developmental stages identified by genetic dissection of pea (Pisum sativum L.) root nodule morphogenesis

Plant Science ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Elena V Morzhina ◽  
Viktor E Tsyganov ◽  
Alexey Y Borisov ◽  
Vladimir K Lebsky ◽  
Igor A Tikhonovich
Keyword(s):  
Pisum Sativum ◽  
Root Nodule ◽  
Developmental Stages ◽  
Genetic Dissection ◽  
Pisum Sativum L

Hugo field pea

2009 ◽  
Vol 89 (1) ◽  
pp. 73-75 ◽  
Author(s):  
Deng-Jin Bing ◽  
Don Beauchesne ◽  
Al Sloan ◽  
Yantai Gan ◽  
Cecil Vera ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Pisum Sativum ◽  
Seed Coat ◽  
Field Pea ◽  
Western Canada ◽  
Seed Shape ◽  
Leaf Type ◽  
Pisum Sativum L ◽  
Cultivar Description ◽  
Round Seed

Hugo is a high-yielding field pea (Pisum sativum L.) cultivar with yellow cotyledons. It has a semi-leafless leaf type, and is powdery mildew resistant. It has round seed shape, medium seed size and high seed coat integrity. The cultivar is adapted to field pea growing regions in western Canada. Key words: Field pea, Pisum sativum, cultivar description

scholarly journals The islet ghrelin cell

2013 ◽  
Vol 52 (1) ◽  
pp. R35-R49 ◽  
Author(s):  
Nils Wierup ◽  
Frank Sundler ◽  
R Scott Heller
Keyword(s):  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Developmental Regulation ◽  
Genetic Regulation ◽  
Cell Types ◽  
Human Islets ◽  
Histochemical Staining ◽  
Pancreatic Tumours ◽  
Age Related ◽  
Staining Methods

The islets of Langerhans are key regulators of glucose homeostasis and have been known as a structure for almost one and a half centuries. During the twentieth century several different cell types were described in the islets of different species and at different developmental stages. Six cell types with identified hormonal product have been described so far by the use of histochemical staining methods, transmission electron microscopy, and immunohistochemistry. Thus, glucagon-producing α-cells, insulin-producing β-cells, somatostatin-producing δ-cells, pancreatic polypeptide-producing PP-cells, serotonin-producing enterochromaffin-cells, and gastrin-producing G-cells have all been found in the mammalian pancreas at least at some developmental stage. Species differences are at hand and age-related differences are also to be considered. Eleven years ago a novel cell type, the ghrelin cell, was discovered in the human islets. Subsequent studies have shown the presence of islet ghrelin cells in several animals, including mouse, rat, gerbils, and fish. The developmental regulation of ghrelin cells in the islets of mice has gained a lot of interest and several studies have added important pieces to the puzzle of molecular mechanisms and the genetic regulation that lead to differentiation into mature ghrelin cells. A body of evidence has shown that ghrelin is an insulinostatic hormone, and the potential for blockade of ghrelin signalling as a therapeutic avenue for type 2 diabetes is intriguing. Furthermore, ghrelin-expressing pancreatic tumours have been reported and ghrelin needs to be taken into account when diagnosing pancreatic tumours. In this review article, we summarise the knowledge about islet ghrelin cells obtained so far.

Carbon-14 Distribution in Peanut Fruit Parts During Maturation Following 14CO Treatment of Intact Plants1

1974 ◽  
Vol 1 (2) ◽  
pp. 63-67 ◽  
Author(s):  
Harold E. Pattee ◽  
Elizabeth B. Johns ◽  
John A. Singleton ◽  
Timothy H. Sanders
Keyword(s):  
Seed Coat ◽  
Developmental Stages ◽  
Specific Activity ◽  
Total Radioactivity ◽  
Maturity Stage ◽  
Early Maturity ◽  
Early Maturation ◽  
Carbon 14 ◽  
Fruit Part ◽  
Successive Feeding

Abstract Effects of sampling date and developmental stage on the distribution of radioactivity within the crude ethanol, lipid, and starch fractions from fruit, seed coat, and seed of peanut were investigated. Major differences were found between the first and fourth feeding dates in the amount of 14C-labeled photo-synthate translocated to individual peanut fruit parts. Maximum levels of radioactivity in the pericarp, seed coat, and seed were attained at progressively later developmental stages as the respective part became the dominant metabolic sink. Within the fruit, maximum radioactivity in starch was reached during early maturity (stage 3) and total radioactivity generally decreased with successive feeding dates. Thus the level of photosynthate being translocated to a given fruit decreases as more fruit develop on the plant. Observed relationships between level of radioactivity and specific activity of fruit-part components were interpreted as indicating that metabolic reserves are built up in the fruit and seed coat during early maturation and utilized later during seed development and maturation when the level of available translocated photosynthate has diminished.

scholarly journals 738 Text ANATOMICAL DESCRIPTION OF THE FRUIT-RECEPTACLE DETACHMENT AREA IN CAYENNE PEPPER

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 538f-538
Author(s):  
Kay P. Gersch ◽  
Carl E. Motsenbocker ◽  
Grezory A. Lang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Developmental Stages ◽  
Histochemical Staining ◽  
Distinct Region ◽  
Cell Type ◽  
Mature Fruit ◽  
Cell Layers ◽  
Or Organization ◽  
Scanning Electron

Two genotypes of cayenne pepper, Capsicum annuun, have been previously identified which differ significantly in ease of fruit detachment force. Both greenhouse- and field-grown plants of these genotypes, Cajun1-9027 and Cap-9004, were investigated for differences in cell type or organization where the fruit and receptacle join. Scanning electron microscopy revealed that mature fruit of genotype Cajun1-9027, which does not separate, exhibits a distinct region of sclerified cells that extend from the periphery of the fruit into the receptacle for at least 15 cell layers. In contrast, mature fruit of the more readily detachable genotype, Cap-9004, had fewer sclerified cells at the point of detachment. Neither genotype exhibits a well-defined abscission zone prior to, or at, maturity. Interpretation of histochemical staining of fruit-receptacle sections following ethylene treatment at different fruit developmental stages will be discussed.

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