Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration

2014 ◽  
pp. 549-555 ◽  
Author(s):  
Hong Qiu ◽  
Wenyuan Xiao ◽  
Jingwen Yue ◽  
Lianchun Wang
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Heparan Sulfate ◽  
Endothelial Cell Migration

Role of cell surface heparan sulfate proteoglycans in endothelial cell migration and mechanotransduction

2005 ◽  
Vol 203 (1) ◽  
pp. 166-176 ◽  
Author(s):  
James J. Moon ◽  
Melissa Matsumoto ◽  
Shyam Patel ◽  
Luke Lee ◽  
Jun-Lin Guan ◽  
...  
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Cell Surface ◽  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycans ◽  
Endothelial Cell Migration

scholarly journals Role of heparan sulfate in mediating CXCL8-induced endothelial cell migration

2015 ◽  
Author(s):  
Zhiping Yan ◽  
Jingxia Liu ◽  
Linshen Xie ◽  
Xiaoheng Liu ◽  
Ye Zeng
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Actin Cytoskeleton ◽  
Heparan Sulfate ◽  
Rho Gtpases ◽  
Umbilical Vein ◽  
Underlying Mechanism ◽  
Endothelial Cell Migration ◽  
Endothelial Glycocalyx

Several positively charged epitopes on the surface of CXCL8 involved in the binding of the major components of endothelial glycocalyx, sulfated glycosaminoglycans (GAGs).In the present study, we aimed to test the hypothesis that the surface GAGs — heparan sulfate (HS) is a crucial prerequisite for enhancement of endothelial cell migration by CXCL8, and to explore its underlying mechanism by detecting the changes in expression of Rho-GTPases and in the organization of actin cytoskeleton after enzymatic removal of HS on human umbilical vein endothelial cells (HUVECs) by using heparinase III.Our results revealed that the reduction of wound area by CXCL8 was greatly attenuated by removal of HS. The upregulations of Rho-GTPases, including Cdc42, Rac1, and RhoA by CXCL8 were suppressed by removal of HS . The polymerization and polarization of actin cytoskeleton, and the increasing of stress fibers by CXCL8 were also abolished by heparinase III. Taken together, our results demonstrated an essential role of HS in mediating CXCL8-induced endothelial cell migration, and highlighted the biological relevance of the CXCL8 and GAGs interactions in endothelial cell migration.

scholarly journals Role of heparan sulfate in mediating CXCL8-induced endothelial cell migration

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1669 ◽  
Author(s):  
Zhiping Yan ◽  
Jingxia Liu ◽  
Linshen Xie ◽  
Xiaoheng Liu ◽  
Ye Zeng
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Actin Cytoskeleton ◽  
Heparan Sulfate ◽  
Rho Gtpases ◽  
Umbilical Vein ◽  
Endothelial Cell Migration

CXCL8 (Interleukin-8, IL-8) plays an important role in angiogenesis and wound healing by prompting endothelial cell migration. It has been suggested that heparan sulfate (HS) could provide binding sites on endothelial cells to retain and activate highly diffusible cytokines and inflammatory chemokines. In the present study, we aimed to test the hypothesis that HS is essential for enhancement of endothelial cell migration by CXCL8, and to explore the underlying mechanism by detecting the changes in expression and activity of Rho GTPases and in the organization of actin cytoskeleton after enzymatic removal of HS on human umbilical vein endothelial cells (HUVECs) by using heparinase III. Our results revealed that the wound healing induced by CXCL8 was greatly attenuated by removal of HS. The CXCL8-upregulated Rho GTPases including Cdc42, Rac1, and RhoA, and CXCL8-increased Rac1/Rho activity were suppressed by removal of HS. The polymerization and polarization of actin cytoskeleton, and the increasing of stress fibers induced by CXCL8 were also abolished by heparinase III. Taken together, our results demonstrated an essential role of HS in mediating CXCL8-induced endothelial cell migration, and highlighted the biological importance of the interaction between CXCL8 and heparan sulfate in wound healing.

scholarly journals Role of heparan sulfate in mediating CXCL8-induced endothelial cell migration

2015 ◽  
Author(s):  
Zhiping Yan ◽  
Jingxia Liu ◽  
Linshen Xie ◽  
Xiaoheng Liu ◽  
Ye Zeng
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Actin Cytoskeleton ◽  
Heparan Sulfate ◽  
Rho Gtpases ◽  
Umbilical Vein ◽  
Underlying Mechanism ◽  
Endothelial Cell Migration ◽  
Endothelial Glycocalyx

Several positively charged epitopes on the surface of CXCL8 involved in the binding of the major components of endothelial glycocalyx, sulfated glycosaminoglycans (GAGs).In the present study, we aimed to test the hypothesis that the surface GAGs — heparan sulfate (HS) is a crucial prerequisite for enhancement of endothelial cell migration by CXCL8, and to explore its underlying mechanism by detecting the changes in expression of Rho-GTPases and in the organization of actin cytoskeleton after enzymatic removal of HS on human umbilical vein endothelial cells (HUVECs) by using heparinase III.Our results revealed that the reduction of wound area by CXCL8 was greatly attenuated by removal of HS. The upregulations of Rho-GTPases, including Cdc42, Rac1, and RhoA by CXCL8 were suppressed by removal of HS . The polymerization and polarization of actin cytoskeleton, and the increasing of stress fibers by CXCL8 were also abolished by heparinase III. Taken together, our results demonstrated an essential role of HS in mediating CXCL8-induced endothelial cell migration, and highlighted the biological relevance of the CXCL8 and GAGs interactions in endothelial cell migration.

Inhibition of endothelial cell migration and angiogenesis by a vascular endothelial growth factor receptor-1 derived peptide

2008 ◽  
Vol 44 (13) ◽  
pp. 1914-1921 ◽  
Author(s):  
Pedro M. Lacal ◽  
Veronica Morea ◽  
Federica Ruffini ◽  
Angela Orecchia ◽  
Annalisa S. Dorio ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Endothelial Cell Migration ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Cyclic strain-mediated regulation of vascular endothelial cell migration and tube formation

2005 ◽  
Vol 329 (2) ◽  
pp. 573-582 ◽  
Author(s):  
Nicholas Von Offenberg Sweeney ◽  
Philip M. Cummins ◽  
Eoin J. Cotter ◽  
Paul A. Fitzpatrick ◽  
Yvonne A. Birney ◽  
...  
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Cyclic Strain ◽  
Tube Formation ◽  
Endothelial Cell Migration ◽  
Vascular Endothelial

scholarly journals Ablation of SGK1 Impairs Endothelial Cell Migration and Tube Formation Leading to Decreased Neo-Angiogenesis Following Myocardial Infarction

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e80268 ◽  
Author(s):  
Elham Zarrinpashneh ◽  
Tommaso Poggioli ◽  
Padmini Sarathchandra ◽  
Jonas Lexow ◽  
Laurent Monassier ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Tube Formation ◽  
Endothelial Cell Migration
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