Effects of High Pressure on Food Proteins

Author(s):  
Jian Yang ◽  
Joseph R. Powers
Keyword(s):  
1997 ◽  
Vol 8 (4) ◽  
pp. 107-112 ◽  
Author(s):  
W. Messens ◽  
J. Van Camp ◽  
A. Huyghebaert
Keyword(s):  

2006 ◽  
Vol 1764 (3) ◽  
pp. 599-618 ◽  
Author(s):  
Eliane Dumay ◽  
Laetitia Picart ◽  
Stéphanie Regnault ◽  
Maryse Thiebaud

1991 ◽  
Vol 55 (5) ◽  
pp. 1253-1257
Author(s):  
Mieko Okamoto ◽  
Hayashi Rikimaru ◽  
Atsushi Enomoto ◽  
Shuichi Kaminogawa ◽  
Kunio Yamauchi

1991 ◽  
Vol 55 (5) ◽  
pp. 1253-1257 ◽  
Author(s):  
Mieko OKAMOTO ◽  
Rikimaru HAYASHI ◽  
Atsushi ENOMOTO ◽  
Shuichi KAMINOGAWA ◽  
Kunio YAMAUCHI

2013 ◽  
Vol 183 ◽  
pp. 19-29 ◽  
Author(s):  
Judit Somkuti ◽  
László Smeller

Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.


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